MARC details
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13349nam a2200337 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20231012100824.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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200727s19uu xx 00 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CvSU Main Campus Library |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
648.5 |
| Item number |
M72 2018 |
| 100 #0 - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Mojica, Ronnel M. |
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author |
| 9 (RLIN) |
38926 |
| 245 10 - TITLE STATEMENT |
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| Title |
Effectiveness of sodium bicarbonate mixed with saba banana (Musa acuminate x balbisiana) and red banana (Musa acuminate Red Dacca) sap as an alternative mordant in gram stain / |
| Statement of responsibility, etc. |
Ronnel M. Mojica. |
| 260 0# - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Indang, Cavite : |
| Date of publication, distribution, etc. |
2018. |
| Name of publisher, distributor, etc. |
Cavite State University- Main Campus, |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xiv, 126 pages: |
| Other physical details |
illustrations ; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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rdacontent |
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text |
| 337 ## - MEDIA TYPE |
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rdamedia |
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unmediated |
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rdacarrier |
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volume |
| 500 ## - GENERAL NOTE |
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Research Study (Bachelor of Science in Medical Technology) Cavite State University. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references. |
| 508 ## - CREATION/PRODUCTION CREDITS NOTE |
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| College and Department |
College of Nursing (CON) |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
EFFECTIVENESS OF SODIUM BICARBONATE MIXED WITH SABA BANANA (Musa acuminata x <br/>balbisiana) AND RED BANANA (Muse acuminata Red Dacca') SAP AS AN ALTERNATIVE MORDANT <br/>IN GRAM STAIN <br/>Research Study <br/>RONNEL M. MOJICA <br/>CAMILLE ANNE B, RODILA <br/>PATRISHIA MAE O, TORRES <br/>College of Nursing <br/>CAVITE STATE UNIVERSITY <br/>Indang, Cavite <br/>May 2018 <br/>EFFECTIVENESS OF SODIUM BICARBONATE MIXED WITH SABA BANANA (Musa acuminata × balbisiana) AND RED BANANA (Musa acuminata 'Red Dacca') SAP AS AN ALTERNATIVE MORDANT IN GRAM STAIN <br/>Undergraduate Research Study Submitted to the Faculty of Department of Medical Technology <br/>College of Nursing <br/>Cavite State University Indang, Cavite <br/>In partial fulfillment of the requirements for the Research MEDT 24B <br/>00017891 <br/>Effectiveness of sodium bicarbonate mixed with saba banana (Musa acuminate x 648.5 M72 2018 <br/>RS-831 <br/>MOJICA, RONNEL M. <br/>RODILA, CAMILLE ANNE B. TORRES, PATRISHIA MAE O. May 2018 <br/>ABSTRACT <br/>MOJICA, RONNEL, RODILA, CAMILLE ANNE, TORRES, PATRISHIA MAE, Effectiveness of Sodium Bicarbonate Mixed with Saba Banana (Musa acuminata × balbisiana) and Red Banana (Musa acuminata 'Red Dacca') Sap as an Alternative Mordant in Gram Stain. Undergraduate Research, Bachelor of Science in Medical Technology. Cavite State University. Indang, Cavite. May 2018. Adviser: Dr. Hosea dL. Matel. <br/>This study aimed to (a) determine the effectiveness of Sodium bicarbonate mixed <br/>with Saba Banana (Musa acuminata × balbisiana) and Red Banana (Musa acuminata 'Red <br/>Dacca') sap can be used as an alternative mordant for gram staining based on color, clarity <br/>and stability;(b) determine the effectiveness of sodium bicarbonate mixed withSaba <br/>Banana (Musa acuminata × balbisiana) and Red Banana (Musa acuminata 'Red Dacca') <br/>sap against Gram's Iodine in microscopic examination. <br/>Two trials, eight treatments, three replicates and a control were used in the study, <br/>eight bacteria were allocated to each treatment. The stained specimen was observed for its <br/>color, clarity and stability. The staining ability of every treatment were developed and <br/>categorized. <br/>Sodium bicarbonate mixed with Saba Banana (Musa acuminata × balbisiana) and <br/>Red Banana (Musa acuminata 'Red Dacca') sap were used as an alternative mordant to <br/>Gram's Iodine, exhibited a good color, clarity and stability. The treatment which has the <br/>best staining ability has a pH of >8 because of its alkalinity. <br/>All concentrations of Sodium bicarbonate mixed with Saba banana (Musa <br/>acuminata × balbisiana) and Red Banana (Musa acuminata 'Red Dacca') sap were highly <br/>V <br/>significant if it will be compared to the staining ability of Gram's Iodine. Saba Banana Sap <br/>(Musa acuminata x balbisiana) has the highest rating compared to Red banana in terms of <br/>mordant color, clarity and stability. The control and treatments exhibit slightly alkaline pH. <br/>Eight bacteria was stained using Gram's Iodine as the control and was also stained using <br/>Sodium bicarbonate mixed with Saba Banana (Musa acuminata × balbisiana) and Red <br/>Banana (Musa acuminata 'Red Dacca') sap which also demonstrate its effectiveness as <br/>compared to Gram's Iodine. <br/>vi <br/>TITLE PAGE... <br/>TABLE OF CONTENTS <br/>Page <br/>i <br/>BIOGRAPHICAL DATA..... <br/>ACKNOWLEDGEMENT. <br/>ABSTRACT............. <br/>TABLE OF CONTENTS... <br/>LIST OF FIGURES.............. <br/>LIST OF APPENDIX FIGURES. <br/>LIST OF APPENDIX TABLES.. <br/>INTRODUTION.. <br/>Objectives of the Study....... <br/>Significance of the Study...................... <br/>Time and Place of the Study... <br/>Scope and Limitation of the Study.......... <br/>Definition of Terms.... <br/>Conceptual Framework. <br/>REVIEW OF RELATED LITERATURE. <br/>Sodium Bicarbonate... <br/>Banana... <br/>Characteristics of Banana... <br/>pH of Banana. <br/>Saba Banana.. <br/>Characteristics of Saba Banana. <br/>ii <br/>111 <br/>V <br/>vii <br/>X <br/>xi <br/>xiii <br/>1 <br/>3 <br/>3 <br/>5 <br/>5 <br/>6 <br/>6 <br/>8 <br/>8 <br/>9 <br/>10 <br/>10 <br/>10 <br/>11 <br/>vii <br/>Health Benefits of Saba Banana... <br/>Red Banana.. <br/>11 <br/>12 <br/>Benefits of Red Banana... <br/>14 <br/>Health Benefits of Red Banana to the Body's Health... <br/>14 <br/>Banana Sap... <br/>15 <br/>Banana Sap in Medicine. <br/>15 <br/>Adverse Effects of Banana... <br/>16 <br/>Banana Sap Dye in Fabrics...... <br/>Work done on Banana pseudostem..... <br/>Banana Sap's Important Constituents.. <br/>Storage... <br/>17 <br/>17 <br/>18 <br/>21 <br/>Gram Staining... <br/>21 <br/>Gram Positive and Gram Negative.... <br/>23 <br/>Biosafety Level 1 Bacteria......... <br/>24 <br/>List of Gram Positive and Gram Negative Bacteria... <br/>25 <br/>Gram Stain Reagents.... <br/>25 <br/>Composition of Grams Iodine...... <br/>27 <br/>Conversion of gram positive cells to gram negative......... <br/>27 <br/>McFarland Standard... <br/>METHODOLOGY... <br/>Research Design... <br/>Preparation of Materials..... <br/>27 <br/>29 <br/>30 <br/>30 <br/>Collection of Plant Material.. <br/>30 <br/>Extraction of Plant Material... <br/>31 <br/>Treatment Preparation.. <br/>33 <br/>viii <br/>Preparation of Bacteria for Staining...... <br/>Smearing Procedure.... <br/>Staining Procedure... <br/>Determination of Staining Properties... <br/>Determination of Stability and Validity... <br/>Criteria for Grading... <br/>Data Gathering...... <br/>Likert Scale... <br/>Proper Disposal of Specimen... <br/>Statistical Analysis...... <br/>RESULTS AND DISCUSSIONS.. <br/>SUMMARY, CONCLUSION AND RECOMMENDATION.. <br/>Summary... <br/>Conclusion. <br/>Recommendations....... <br/>REFERENCES... <br/>APPENDICES.. <br/>ix <br/>33 <br/>33 <br/>34 <br/>35 <br/>35 <br/>37 <br/>38 <br/>39 <br/>40 <br/>40 <br/>41 <br/>67 <br/>67 <br/>70 <br/>72 <br/>70 <br/>2 2 2 <br/>76 <br/>LIST OF FIGURES <br/>Figure <br/>Page <br/>1 Conceptual Framework of the Study....... <br/>2 Illustration of the Grading System... <br/>3 Flowchart of Methodology.. <br/>4 Schematic diagram of the study.. <br/>5 Gram's staining procedure.. <br/>6 Process of preparing the banana sap. <br/>7 Collection and Processing of Plant Material. <br/>8 Extraction and Processing of Banana Sap.... <br/>7 <br/>39 <br/>78 <br/>79 <br/>80 <br/>81 <br/>82 <br/>83 <br/>9 Treatment Preparation...... <br/>84 <br/>10 Bacterial Preparation McFarland Standard.. <br/>85 <br/>11 Bacterial Smearing Procedure.. <br/>86 <br/>12 Gram Staining Procedure..... <br/>87 <br/>13 Decontamination Procedures.. <br/>88 <br/>X <br/>LIST OF APPENDIX FIGURES <br/>Figure <br/>1 Flowchart of methodology <br/>Page <br/>78 <br/>2 Schematic diagram of the study <br/>3 Gram staining procedure <br/>4 Process of preparing the banana sap <br/>5 Collection and Processing of Plant Material <br/>6 Extraction and Processing of Banana Sap <br/>7 Treatment Preparation <br/>8 Bacterial Preparation McFarland Standard <br/>9 Bacterial Smearing Procedure <br/>79 <br/>80 <br/>81 <br/>82 <br/>83 <br/>84 <br/>85 <br/>86 <br/>10 Gram Staining Procedure.. <br/>87 <br/>88 <br/>11 Decontamination Procedures <br/>12 Staphylococcus aureus using Sodium Bicabonate <br/>mixed with Saba Banana Sap <br/>89 <br/>13 Staphylococcus aureus using Sodium Bicarbonate <br/>mixed with Red Banana Sap. <br/>90 <br/>14 Staphylococcus epidermidis using Sodium Bicarbonate <br/>mixed with Saba Banana Sap <br/>91 <br/>15 Staphylococcus epidermidis using Sodium Bicarbonate <br/>mixed with Red Banana Sap. <br/>92 <br/>16 Bacillus subtilis using Sodium Bicarbonate <br/>mixed with Saba Banana Sap <br/>93 <br/>17 Bacillus subtilis using Sodium Bicarbonate <br/>mixed with Red Banana Sap <br/>xi <br/>94 <br/>18 Bacillus coagulans using Sodium Bicarbonate <br/>mixed with Saba Banana Sap <br/>19 Bacillus coagulans using Sodium Bicarbonate <br/>mixed with Red Banana Sap <br/>95 <br/>96 <br/>20 Enterobacter aerogenes using Sodium Bicarbonate <br/>mixed with Saba Banana Sap <br/>97 <br/>21 Enterobacter aerogenes using Sodium Bicarbonate <br/>mixed with Red Sap <br/>98 <br/>22 Escherichia coli using Sodium Bicarbonate <br/>mixed with Saba Banana Sap <br/>99 <br/>23 Echerichia coli using Sodium Bicarbonate <br/>mixed with Red Banana Sap <br/>100 <br/>24 Serratia marcescens using Sodium Bicarbonate <br/>mixed with Saba Banana Sap.. <br/>101 <br/>25 Serratia marcescens using Sodium Bicarbonate <br/>mixed with Red Banana Sap <br/>102 <br/>26 Pseudomonas aeruginosa using Sodium Bicarbonate <br/>mixed with Saba Banana Sap <br/>103 <br/>27 Pseudomonas aeruginosa using Sodium Bicarbonate <br/>mixed with Red Banana Sap <br/>104 <br/>Xii <br/>LIST OF APPENDIX TABLES <br/>Table <br/>1 Mean scores for color in tests of Between-Subjects <br/>Effects for Staphylococcus aureus... <br/>2 Staphylococcus aureus mean scores for color... <br/>3 Mean scores for clarity in tests of Between-Subjects <br/>Effects for Staphylococcus aureus...... <br/>4 Staphylococcus aureus mean scores for clarity... <br/>Page <br/>105 <br/>105 <br/>106 <br/>106 <br/>5 Mean scores for color in tests of Between-Subjects <br/>Effects for Staphylococcus epidermidis... <br/>107 <br/>6 Staphylococcus epidermidis mean scores for color... <br/>107 <br/>7 Mean scores for clarity in tests of Between-Subjects <br/>Effects for Staphylococcus epidermidis......... <br/>108 <br/>8 Staphylococcus epidermidis mean scores for clarity... <br/>108 <br/>9 Mean scores for color in tests of Between-Subjects <br/>Effects for Bacillus subtilis... <br/>109 <br/>10 Bacillus subtilis mean scores for color.... <br/>109 <br/>11 Mean scores for clarity in tests of Between-Subjects <br/>Effects for Bacillus subtilis... <br/>110 <br/>12 Bacillus subtilis mean scores for clarity... <br/>110 <br/>13 Mean scores for color in tests of Between-Subjects <br/>Effects for Bacillus coagulans... <br/>111 <br/>14 Bacillus coagulans mean scores for color... <br/>111 <br/>15 Mean scores for clarity in tests of Between-Subjects <br/>Effects for Bacillus coagulans.......... <br/>112 <br/>16 Bacillus coagulans mean scores for clarity.. <br/>112 <br/>xiii <br/>17 Mean scores for color in tests of Between-Subject <br/>Effects for Enterobacter aerogenes... <br/>18 Enterobacter aerogenes mean scores for color. <br/>19 Mean scores for clarity in tests of Between-Subjects <br/>Effects for Enterobacter aerogenes... <br/>20 Enterobacter aerogenes mean scores for clarity... <br/>21 Mean scores for color in tests of Between-Subjects <br/>Effects for Escherichia coli... <br/>113 <br/>113 <br/>114 <br/>114 <br/>115 <br/>22 Escherichia coli mean scores for color... <br/>115 <br/>23 Mean scores for clarity in tests of Between-Subjects <br/>Effects for Escherichia coli... <br/>116 <br/>24 Escherichia colimean scores for clarity......... <br/>116 <br/>25 Mean scores for color in tests of Between-Subjects <br/>Effects for Serratia marcescens... <br/>117 <br/>26 Serratia marcescens mean scores for color.. <br/>117 <br/>27 Mean scores for clarity in tests of Between-Subjects <br/>Effects for Serratia marcescens... <br/>118 <br/>118 <br/>28 Serratia marcescens mean scores for clarity...... <br/>29 Mean scores for color in tests of Between-Subjects <br/>Effects for Pseudomonas aeruginosa... <br/>30 Pseudomonas aeruginosamean scores for color.. <br/>31 Mean scores for clarity in tests of Between-Subjects <br/>Effects for Pseudomonas aeruginosa... <br/>32 Pseudomonas aeruginosamean scores for clarity.. <br/>xiv <br/>119 <br/>119 <br/>120 <br/>120 <br/>EFFECTIVENESS OF SODIUM BICARBONATE MIXED WITH SABA <br/>BANANA (Musa acuminata × balbisiana) AND RED BANANA (Musa acuminata 'Red Dacca') SAP AS AN ALTERNATIVE MORDANT IN GRAM STAIN <br/>MOJICA, RONNEL M RODILA, CAMILLE ANNE B. TORRES, PATRISHIA MAE O. <br/>An undergraduate research study submitted to the faculty of the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite in partial fulfillment of the requirements for the degree of Bachelor of Science in Medical Technology with Contribution No. <br/>Prepared under the supervision <br/>of Hosea dL. Matel, PhD. and Charmaine V. Rosales, RMT. <br/>INTRODUCTION <br/>Sodium bicarbonate is a white, crystalline powder that is commonly used <br/>as a pH buffering agent, an electrolyte replenisher and systemic alkalizer. It has a pH of <br/>8-9, non-toxic and bitter taste. In microbiology, it is used as an assistant to mordant stain. <br/>It may be added just before use to intensify the uptake of color. <br/>Banana sap <br/>is a well-known extract that can cause stain which cannot be <br/>easily removed with bleach and soap. It is extremely sticky which can be used as an <br/>alternative adhesive. Sap can be obtained from different parts of the banana tree. It has <br/>some special properties relating to various phenomena such as browning of fruits after <br/>harvesting caused by its polyphenol oxidase constituent and also permanent staining of <br/> |
| 541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE |
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| Method of acquisition |
Submitted to the University Library |
| Date of acquisition |
07/16/2018 |
| Accession number |
RS-831 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Sodium bicarbonate |
| 9 (RLIN) |
34488 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Banana |
| 9 (RLIN) |
20805 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) |
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| 9 (RLIN) |
1856 |
| Course or Program |
Bachelor of Science in Medical Technology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rodila, Camille Anne B. |
| 9 (RLIN) |
34489 |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Torres, Patricia Mae O. |
| 9 (RLIN) |
34490 |
| Relator term |
author |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Port |
80 |
| Link text |
Click here to view thesis abstract and table of contents |
| Electronic File |
<a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=c113efe7beb3fff5548e337e8b5ce860">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=c113efe7beb3fff5548e337e8b5ce860</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Theses / Manuscripts |
