MARC details
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03903nam a2200361 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20211114183243.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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210304b ||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CvSU Main Campus Library |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
399.0113 |
| Item number |
B46 2018 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
4626 |
| Personal name |
Berchie, Emmanuel A. |
| Relator term |
adviser |
| 245 10 - TITLE STATEMENT |
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| Title |
Hemagglutinating activity of birch flower (Broussonetia luzonical) lectin in abo blood group / |
| Statement of responsibility, etc. |
by Emmanuel A. Berchie, Keithlyn P. Gutierrez, and Abegail P. Rabanzo. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Indang, Cavite : |
| Name of publisher, distributor, etc. |
Cavite State University- Main Campus, |
| Date of publication, distribution, etc. |
2018. |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xvi, 87 pages : |
| Other physical details |
illustrations ; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 500 ## - GENERAL NOTE |
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| General note |
Thesis (Bachelor of Science in Medical Technology) Cavite State University |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references. |
| 508 ## - CREATION/PRODUCTION CREDITS NOTE |
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| College and Department |
College of Nursing (CON), Department of Medical Technology |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
BERCHIE, EMMANUEL A., GUTIERREZ, KEITHLYN P., RABANZO, ABEGAIL P. Hemagglutinating Activity of Birch flower (Broussonetia luzonica) Lectin in ABO Blood Group. Research Study. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. May 2018, Adviser: Hosea L. Matel, PhD. and Annie M. Ramos, RMT, RN, MD. <br/><br/>The extensive application and variety of uses of lectins express the necessity to isolate lectins from indigenous sources since lectins are very expensive. One of the possible native sources of lectin is the birch flower (Broussonetia luzonica) which is commonly known as "himbabao". The study was conducted to determine the presence or absence of hemagglutination in birch flower extracts using microtiter plate (MTP) — based hemagglutination; determine the strength of agglutination of ABO blood antigen through microtiter plate (MTP) — based hemagglutination; characterize the hemagglutinating activity of lectin obtained from birch flower in terms of human blood type specificity and effect of storage time on agglutination activity; and determine significant difference between the microtiter plate (MTP) — based hemagglutination of extracts of O. 15M NaC1 in IX PBS and phosphate buffer saline (PBS) solution. <br/><br/>In this research, the lectin was extracted using 0.15M sodium chloride (NaCI) in lX phosphate buffered saline (PBS) solution and PBS solution alone. The isolation and purification of lectin was done by ammonium sulfate precipitation at 40% to 80% saturation. Microliter plate (MTP) P) based hemagglutination using human ABO erythrocytes was performed in order to determine the presence of lectin. Consequently, the birch flower lectin was categorized as a non-blood type specific lectin since it agglutinated all the human blood types A, B, 0 and AB. The 80(') ammonium sulfate precipitate accompanied by 0.15M NaCl in IX 1)13S gave highest titer value among the other extracts with 2048 in type 13 and AB, followed by type A with 1024. The stability of lectin activity was determined after using, protein fractions with one-week, one-month and two-month shelf life. The peak of the lectin activity shown in the study was observed after one month storage of the protein fractions and gradual loss of activity was noted after second month of storage under refrigeration.<br/><br/> |
| 541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE |
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| Method of acquisition |
Submitted to the University Library |
| Date of acquisition |
May 27, 2019 |
| Accession number |
RS-849 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
4627 |
| Topical term or geographic name entry element |
Blood |
| General subdivision |
Agglutination |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
4628 |
| Topical term or geographic name entry element |
Lectins |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) |
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| 9 (RLIN) |
1856 |
| Course or Program |
Bachelor of Science in Medical Technology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
4630 |
| Personal name |
Gutierrez, Keithlyn P. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
4631 |
| Personal name |
Rabanzo, Abegail P. |
| Relator term |
author |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
1609 |
| Personal name |
Matel, Hosea dL. |
| Relator term |
adviser |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
2555 |
| Personal name |
Ramos, Annie M. |
| Relator term |
adviser |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Port |
80 |
| Link text |
Click here to view abstract and table of contents |
| Electronic File |
<a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=70f7e0fb23c6d1a9e26b650d223a9189">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=70f7e0fb23c6d1a9e26b650d223a9189</a> |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Theses / Manuscripts |
