Antibacterial activity of Pandacaqui Puti (Tabernaemontana Pandacaqui Poir) plant parts extract against staphylococcus and Escherichia coli / (Record no. 34082)

MARC details
000 -LEADER
fixed length control field 05169nam a2200349 4500
003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220316145243.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 210729b ||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency CvSU Main Campus Library
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 615
Item number Ar2 2018
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 8768
Personal name Arca, Ermie Jay B.
Relator term author
245 10 - TITLE STATEMENT
Title Antibacterial activity of Pandacaqui Puti (Tabernaemontana Pandacaqui Poir) plant parts extract against staphylococcus and Escherichia coli /
Statement of responsibility, etc. by Ermie Jay B. Arca and Clarize Z. Candalo.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Indang, Cavite :
Name of publisher, distributor, etc. Cavite State University- Main Campus,
Date of publication, distribution, etc. 2018.
300 ## - PHYSICAL DESCRIPTION
Extent xii, 76 pages :
Other physical details illustrations ;
Dimensions 28 cm.
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
500 ## - GENERAL NOTE
General note Research Study (Bachelor of Science in Medical Technology) Cavite State University.
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references.
508 ## - CREATION/PRODUCTION CREDITS NOTE
College and Department College of Nursing (CON), Department of Medical Technology
520 3# - SUMMARY, ETC.
Summary, etc. ARCA, ERMIE JAY B., CANDADO, CLARICE Z. Antibacterial Activity Of Pandakaki Puti (Tabernaemontana Pandacaqui Poir) Plant Parts Extract Against Staphylococcus Aureus and Escherichia Coll. Undergraduate Research Study. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. May 2018. <br/>This study on the antibacterial activity of T. pandacaquiPoirplant parts extract aimed to determine the concentration of the plant parts extract that inhibit bacterial growth of the S. aureus and E. coli in terms of inhibition zones; to determine the significant differences among the inhibition zones caused by the positive control and the leaf and stem extracts; and to determine the minimum inhibitory concentration of the plant part extract that caused the highest inhibition zone. The antibacterial activity of T. pandacaqui Poir was laid out in a Completely Randomized Design (CRD) with four replications of the different treatments of the leaf and stem extracts of T. pandacaqui Poir. The antibacterial activity was done using Kirby-Bauer Test (Disk Diffusion Method). Five treatments were prepared for leaf and stem extracts separately; 0.1 g/ m1 ,0.2 g/ml, 0.3g/ml, 0.4 glml and 0.5 g/ml, respectively. On the other hand, the test organisms, namely, Staphylococcus aureus and Escherichia coli, were purchased from Philippine National Collection of Microorganism, University of the Philippines Los Banos. The test organisms were sub cultured and standardized. The filter paper impregnated with the different concentration of leaf and stem extracts were placed onto the inoculated surface of the Mueller Hinton Agar (MHA) and pressed firmly and were incubated. After 24 hours of incubation, the zones of inhibition were measured and interpreted using the Clinical Laboratory Standard Institute (CLSI) standard. On the plates with S. aureus, the concentrations of leaf extracts that yielded a Resistant interpretation were the 0.1 g/ml, 0.4 g/m1 and 0.5 g/ml with inhibition zones of 12,25 mm, 12 mm and 10 mm, respectively. The leaf extracts that yielded an Intermediate interpretation were the 0.2 g/m1 and 0.3 g/ml with inhibition zones of 14.5 mm and 14.25 mm. The stem extracts that exhibited a resistant interpretation were 0.1 g/ml, 0.2 g/ml and 0.4 g/ml with inhibition zones of 10.75 mm, 11.25 mm and 13.5 mm, respectively. Concentrations with Intermediate interpretation were 0.3 g/ml and 0.5 g/ml with inhibition zones of 14.15 mm and 15 mm. The 0.3 g/ml and 0.5 g/m1 leaf extract were interpreted as Resistant with inhibition zones of 13.75 mm and 6.75 mm. The Intermediate inhibition zones were 0.1 g/ml and 0.2 g/ml with inhibition zones of 14.5 mm and 15.75 mm. The 0.4 g/ml leaf extract was interpreted as Susceptible with an inhibition zone of 22.75 mm. On the other hand, the stem extracts with concentrations of 0.1 g/ml, 0.2 g/ml, 0.3 g/ml, 0.4 g/ml and 0.5 g/ml were interpreted as Resistant with inhibition zones of 10.5 mm, 9.75 mm, 9.75 mm, 9.5 mm and 9.25 mm, respectively. The plant part extracts that exhibited the highest zone of inhibition were subjected to Minimum Inhibitory Concentration (MIC). The 0.2 g/ml leaf extract and 0.5 g/ml stem extract were evaluated for its minimum inhibitory concentration against S. aureus. On the other hand, 0.4 g/ml leaf extract and 0.1 g/ml stem extract were applied to E. coil in varying concentrations. The varying concentrations of 0.2 g/ml leaf extract, 0.5 g/ml leaf extract and 0.1 g/ml stem extract did not caused inhibition of bacterial growth while the 0.4 g/ml leaf extract inhibited bacterial growth.<br/>
541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE
Method of acquisition Submitted to the University Library
Date of acquisition July 16,2018
Accession number RS-823
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 3620
Topical term or geographic name entry element Herbs
General subdivision Therapeutic use
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 8770
Topical term or geographic name entry element Antibacterial agents
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 8771
Topical term or geographic name entry element Bacterial disease
General subdivision Alternative
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
9 (RLIN) 1856
Course or Program Bachelor of Science in Medical Technology
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 8772
Personal name Candalo, Clarize Z.
Relator term author
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 4553
Personal name Sangalang, Adelaida E.
Relator term adviser
856 ## - ELECTRONIC LOCATION AND ACCESS
Electronic File <a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=3968c4058f1ec98092866ffa94bbb212">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=3968c4058f1ec98092866ffa94bbb212</a>
Link text Click here to view thesis abstract and table of contents
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Theses / Manuscripts
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Home library Current library Shelving location Date acquired Source of acquisition Coded location qualifier Inventory number Total Checkouts Full call number Barcode Date last seen Uniform Resource Identifier Price effective from Koha item type Public note
    Dewey Decimal Classification   Room use only Non-fiction Ladislao N. Diwa Memorial Library Ladislao N. Diwa Memorial Library Theses Section 07/16/2018 Submitted copy to the University Library RS 600   615 Ar2 2018 00079793 07/29/2021 http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=3968c4058f1ec98092866ffa94bbb212 07/29/2021 Theses / Manuscripts RS-823
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