MARC details
| 000 -LEADER |
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| fixed length control field |
03124nam a22002897a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
|---|
| control field |
OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20220421102543.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
211201b |||||||| |||| 00| 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9789386355126 (hardcover) |
| 040 ## - CATALOGING SOURCE |
|---|
| Transcribing agency |
CvSU Main Campus Library |
| Description conventions |
rda |
| 041 ## - LANGUAGE CODE |
|---|
| Language code of text/sound track or separate title |
eng |
| 050 ## - LIBRARY OF CONGRESS CALL NUMBER |
|---|
| Classification number |
TP248.22 |
| Item number |
Si6 2017 |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
|---|
| 9 (RLIN) |
15067 |
| Personal name |
Singh, K. P. |
| Relator term |
author |
| 245 ## - TITLE STATEMENT |
|---|
| Title |
Laboratory techniques in biotechnology : |
| Remainder of title |
methods and applications / |
| Statement of responsibility, etc. |
Dr. K. P. Singh. |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
|---|
| Place of publication, distribution, etc. |
New Delhi, India : |
| Name of publisher, distributor, etc. |
Random Publications, |
| Date of publication, distribution, etc. |
2017 |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
302 pages : |
| Other physical details |
illustrations ; |
| Dimensions |
24 cm |
| 336 ## - CONTENT TYPE |
|---|
| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
|---|
| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
|---|
| Source |
rdacarrier |
| Carrier type term |
volume |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
|---|
| Bibliography, etc. note |
Includes bibliographical references and index. |
| 505 ## - FORMATTED CONTENTS NOTE |
|---|
| Formatted contents note |
Introduction -- Laboratory to field -- Laboratory techniques in biotechnology -- Recombinant DNA technology -- Patenting hybrids and chimeras -- Stem cell technology -- DNA and RNA -- Environment and biological treatment technology -- Biotechnology and genomics sequence. <br/> |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc. |
"Genetic engineering deals with synthesis of artificial gene, repair of gene, combining of DNA from two organism (recombinant DNA) and manipulating the artificial gene together with the recombinant DNA for the improvement of microbes, plants, animals & human being. Stem cell technology is a rapidly developing field that combines the efforts of cell biologists, geneticists, and clinicians and offers hope of effective treatment for a variety of malignant and non-malignant diseases. Stem cells are defined as totipotent progenitor cells capable of self renewal and multilineage differentiation. 1. Stem cells survive well and show stable division in culture, making them ideal targets for in vitro manipulation. To study or manipulate nucleic acids, the DNA or RNA must first be isolated or extracted from the cells. This can be done through various techniques. Most nucleic acid extraction techniques involve steps to break open the cell and use enzymatic reactions to destroy all macromolecules that are not desired (such as degradation of unwanted molecules and separation from the DNA sample). Cells are broken using a lysis buffer (a solution that is mostly a detergent); lysis means “to split”. These enzymes break apart lipid molecules in the membranes of the cell and the nucleus. Macromolecules are inactivated using enzymes such as proteases that break down proteins, and ribonucleases (RNAses) that break down RNA. His book provide adequate background and current information on recent biotechnological tools and techniques and its application. It will be very useful for a variety of scholars, biotechnologists, medical doctors, researchers, teachers and students in the field of biotechnology."--Back cover |
| 541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE |
|---|
| Source of acquisition |
Fund 164 |
| Vendor |
BookQuick Marketing |
| Method of acquisition |
Purchased |
| Date of acquisition |
10/31/2019 |
| Accession number |
78119 |
| Owner |
NEJ |
| Purchase price |
PHP 6,295.50 |
| PO No. |
2019-10-818 |
| ICS or PAR No. |
2019-1-0678 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| 9 (RLIN) |
2083 |
| Topical term or geographic name entry element |
Biotechnology |
| General subdivision |
Study and teaching |
| Geographic subdivision |
India |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
|---|
| Electronic File |
<a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=9194d8c428ee19860769b295cd6663e5">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=9194d8c428ee19860769b295cd6663e5</a> |
| Link text |
Click here to view the table of contents |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Library of Congress Classification |
| Koha item type |
Books |
