Antibiofilm and cytotoxic activity of pungapung (Amorphophallus campanulatus Roxb.) extracts / (Record no. 60612)

MARC details
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003 - CONTROL NUMBER IDENTIFIER
control field OSt
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220714135349.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 220502b |||||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Transcribing agency CvSU Main Campus Library
041 0# - LANGUAGE CODE
Language code of text/sound track or separate title eng
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 616.904
Item number V71 2019
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 22907
Personal name Villanueva, Dhenicka Vivien C.
Relator term author
245 10 - TITLE STATEMENT
Title Antibiofilm and cytotoxic activity of pungapung (Amorphophallus campanulatus Roxb.) extracts /
Statement of responsibility, etc. by Dhenicka Vivien C. Villanueva.
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Indang, Cavite :
Name of publisher, distributor, etc. Cavite State University- Main Campus,
Date of publication, distribution, etc. 2019.
300 ## - PHYSICAL DESCRIPTION
Extent xv, 125 pages :
Other physical details illustrations ;
Dimensions 28 cm.
336 ## - CONTENT TYPE
Source rdacontent
Content type term text
337 ## - MEDIA TYPE
Source rdamedia
Media type term unmediated
338 ## - CARRIER TYPE
Source rdacarrier
Carrier type term volume
500 ## - GENERAL NOTE
General note Thesis (Bachelor of Science in Biology) Cavite State University.
504 ## - BIBLIOGRAPHY, ETC. NOTE
Bibliography, etc. note Includes bibliographical references.
508 ## - CREATION/PRODUCTION CREDITS NOTE
College and Department College of Arts and Sciences (CAS), Department of Biological Sciences
520 3# - SUMMARY, ETC.
Summary, etc. VILLANUEVA, DHENICKA VIVIEN CABREROS. ANTIBIOFILM AND CYTOTOXIC ACTIVITY OF PUNGAPUNG (Amorphophallus campanulatus Roxb.) EXTRACTS. Undergraduate Thesis, Bachelor of Science in Biology. Cavite State University, Indang, Cavite. June 2019. Adviser: Dr. Yolanda A. Ilagan.<br/><br/>A study was conducted to assess the antibacterial, antibiofilm, and cytotoxic activities of pungapung (Amorphophallus campanulatus Roxb.) against Escherichia coli B1825, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 12600.<br/><br/>Leaves and stems of A. campanulatus were subjected to different extraction procedures: decoction, crude juice, dichloromethane, and methanolic extracts and were tested against these test organisms. Disk-diffusion assay showed that only decoction and methanolic extracts exhibited antibacterial activity against E. coli. Minimum inhibitory concentration (MIC) of these extracts were determined using resazurin-based microtiter plate assay. The MIC for decoction and methanolic extracts were observed at 50 mg/ml and 40 mg/ml against E. coli. The extracts were then tested for antibiofilm activity using biofilm inhibitory assay in different wavelengths (450 nm, 490 nm, and 630 nm). Analysis of Variance (ANOVA) revealed that decoction and methanolic extracts were better than gentamicin in inhibitory biofilm formation. Cytotoxic activity of the extracts was determined using brine shrimp lethality assay (Artemia salina) using various concentrations. Results revealed that the highest death percentage (95.56%) was caused by methanolic stem extract followed by methanolic leaf extract (86.67%) at 1000 ppm concentration. The estimated LCso value based on probit analysis after six hours was 10.09 ppm. Decoction extracts were considered less toxic than methanolic extracts.<br/><br/><br/>
541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE
Method of acquisition Submitted to the University Library
Date of acquisition 07/18/2019
Accession number T-8344
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 12439
Topical term or geographic name entry element Anti-Bacterial Agents
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 22908
Topical term or geographic name entry element Antiviral agents
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 22909
Topical term or geographic name entry element Anti-Infective Agents, Local
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
9 (RLIN) 1672
Course or Program Bachelor of Science in Biology
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 22
Personal name Ilagan, Yolanda A.
Relator term adviser
856 ## - ELECTRONIC LOCATION AND ACCESS
Electronic File <a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=ae0b6ad84cc059c4c7a525261a1cfb78">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=ae0b6ad84cc059c4c7a525261a1cfb78</a>
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942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Theses / Manuscripts
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Collection code Home library Current library Shelving location Date acquired Source of acquisition Coded location qualifier Inventory number Total Checkouts Full call number Barcode Date last seen Uniform Resource Identifier Price effective from Koha item type Public note
    Dewey Decimal Classification   Room use only Non-fiction Ladislao N. Diwa Memorial Library Ladislao N. Diwa Memorial Library Theses Section 07/18/2019 Submitted copy for the University Library THESIS/SP 600   616.904 V71 2019 00079650 05/02/2022 http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=ae0b6ad84cc059c4c7a525261a1cfb78 05/02/2022 Theses / Manuscripts T-8344
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