MARC details
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04765nam a2200313 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20230422104411.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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070508s19uu xx 00 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CvSU Main Campus Library |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
636.214 |
| Item number |
N88 2005 |
| 100 #0 - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Nuestro, Ma. Clarissa Angela S. |
| 9 (RLIN) |
10579 |
| Relator term |
author |
| 245 10 - TITLE STATEMENT |
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| Title |
Detection and prevalence of shiga (vero) toxin producing E-coli 0157 in different age groups of dairy cattle from the Batangas Dairy Cooperative (BADACO) |
| Statement of responsibility, etc. |
by Ma. Clarissa Angela S. Nuestro |
| 260 0# - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Indang, Cavite : |
| Date of publication, distribution, etc. |
2005. |
| Name of publisher, distributor, etc. |
Cavite State University- Main Campus, |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
xvii, 59 pages : |
| Other physical details |
illustrations ; |
| Dimensions |
28 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 500 ## - GENERAL NOTE |
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| General note |
Thesis (Doctor of Veterinary Medicine) Cavite State University |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references. |
| 508 ## - CREATION/PRODUCTION CREDITS NOTE |
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| College and Department |
College of Veterinary Medicine and Biomedical Sciences (VETMET) |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
NUESTRO, MA. CLARISSA ANGELA S., Cavite State University, Indang, Cavite. April 2005. Detection and Prevalence of Shiga (Vero) Toxin-Producing Escherichia coli 0157 in Different Age Groups of Dairy Cattle (Bos Taurus) from the Batangas Dairy Cooperative (BADACO). Adviser: Ma. Cynthia N. Rundina, DVM, MS. <br/>This study was conducted primarily to detect and establish the prevalence of Shiga (Vero) toxin-producing Escherichia coli 0157 from the rectal fecal samples of 125 apparently healthy dairy cattle (Bos taurus) belonging to different age groups. Specifically, it aimed to isolate Shiga (Vero) toxin-producing E. coli 0157 by direct culture and characterize the isolates morphologically, biochemically and serologically. Finally, Shiga (Vero) toxin detection was done by the use of the Polymerase Chain Reaction (PCR) using slt-II primer. The rectal fecal samples were all subjected to direct culture onto Cefixime Tellurite-Sorbitol MacConkey Agar (CT-SMAC). In this medium a total of 61 (48.8%) non-sorbitol fermenting colonies, which was considered as presumptive Enterohemorrhagic Escherichia coli (EHEC) were isolated: 21 (34.4%) from calves, 15 (24.6%) in yearlings, 16 (26.2%) from heifers and 9 (14.8%) from cows. The 61 presumptive EHEC isolates were then tested for cytochrome oxidase activity and only 23 (37.7%) produced negative results. These were further characterized morphologically and biochemically and all were found to be gram negative and rod-shaped organisms. Biochemically, the isolates produced the following typical reactions: Acid slant/Acid butt with gas production (A/A gas) in the Triple Sugar Iron (TSI) agar, Voges Proskauer test negative (-), Urease negative (-), Citrate negative (-), no hydrogen sulfide produced and motile in Sulfide Indole Motility (SIM) medium, Indole positive (+) and Methyl Red positive (+) and Lysine Decarboxylase positive (+). The highest number of presumptive EHEC isolates was obtained from the age group of calves, having 10 (43.4%) while the least number of isolates was obtained from heifers, having 3 (13%) isolates. Three out of 23 (13%) of the presumptive EHEC did not ferment sorbitol in the sorbitol utilization test. These isolates were considered as presumptive Enterohemorrhagic Escherichia coli and were subjected to agglutination with 0157 antiserum. None of the samples agglutinated with the 0157 antiserum. Further test using the Polymerase Chain Reaction (PCR) of the BIOTECH 12 and BIOTECH 13 primers for E. coli 0157 also showed that none of the samples amplified the primers. Polymerase Chain Reaction using slt-II primer on the other hand, yielded 2 isolates which did not amplify the primer whereas, 1 isolate amplified the primer. These results showed that none of the isolates were E. coli 0157:H7 although one sample probably possessed the slt-II gene. This study showed that the EHEC strain E. coli 0157:H7 may not be present in the dairy cattle sampled in this study. However, these results could indicate that other EHEC strains may be present and as such similar studies on equally important EHEC strains are warranted. Moreover, based on the results of the PCR, non-E. coli 0157:H7 isolates are also capable of producing Shiga (Vero) toxins. Therefore, similar precautionary measures on handling of cattle as well as produce from these animals are <br/>also necessary. <br/><br/> |
| 541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE |
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| Method of acquisition |
Submitted to the University Library |
| Date of acquisition |
07/18/2007 |
| Accession number |
T-2974 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Dairy cattle |
| 9 (RLIN) |
10580 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) |
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| 9 (RLIN) |
66 |
| Course or Program |
Doctor of Veterinary Medicine |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Rundina- dela Cruz, Cynthia N. |
| 9 (RLIN) |
29399 |
| Relator term |
adviser |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Electronic File |
<a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=18b2c97c2f9a4720440b5d914555208f">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=18b2c97c2f9a4720440b5d914555208f</a> |
| Link text |
Click here to view abstract and table of contents |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Theses / Manuscripts |
