MARC details
| 000 -LEADER |
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| fixed length control field |
02928nam a2200325 a 4500 |
| 003 - CONTROL NUMBER IDENTIFIER |
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| control field |
OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20230503140505.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
071128s19uu xx 00 eng d |
| 040 ## - CATALOGING SOURCE |
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| Transcribing agency |
CvSU Main Campus Library |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Classification number |
574.873 |
| Item number |
B14 2007 |
| 100 #0 - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Bago, Edison D. |
| 9 (RLIN) |
13506 |
| Relator term |
author |
| 245 10 - TITLE STATEMENT |
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| Title |
Development of a protocol for the isolation of plant DNA from coffee and pechay / |
| Statement of responsibility, etc. |
by Edison D. Bago. |
| 260 0# - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
Indang, Cavite : |
| Date of publication, distribution, etc. |
2007. |
| Name of publisher, distributor, etc. |
Cavite State University- Main Campus, |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
x, 34 pages : |
| Dimensions |
28 cm. |
| Other physical details |
illustrations ; |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 500 ## - GENERAL NOTE |
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| General note |
Thesis (BS Biology) Cavite State University. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc. note |
Includes bibliographical references. |
| 508 ## - CREATION/PRODUCTION CREDITS NOTE |
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| College and Department |
College of Arts and Science (CAS) |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc. |
BAGO, EDISON D. Development of a Protocol for the Isolation of Plant DNA from Coffee (Coffea liberica) and Pechay (Brassica chinensis). Undergraduate Thesis. Bachelor of Science in Biology major in General Biology. Cavite State University, Indang, Cavite. April 2007. Adviser: Dr. Yolanda A. Hagan <br/>The study was conducted at Biotechnology Laboratory of the Biological Science Department from October 2006 to January 2007. This study aimed develop a plant DNA isolation protocol suitable for both softwood and hardwood plant species. The used protocols were based on Edwards et al. (1991) and Doyle and Doyle (1990) protocoL The key steps for the modified Edwards et al. (1991) protocol were: 1) extraction with isolation buffer containing SDS (sodium dodecyl sulfate); and 2) precipitation of DNA by isopropanol. Modifications employed on different plant samples were the change in the incubation temperature on both key steps. Electrophoretic analysis showed that it is unsuccessful in extracting DNA in both pechay and coffee. The key steps for the modified Doyle and Doyle (1990) protocol were: (1) extraction with high salt CTAB buffer; (2) chloroform isoamyl alcohol extraction; (3) isopropyl alcohol precipitation, and; (4) cleaning and washing with ethanol. Modifications employed on different plant samples were the change in incubation temperature for CTAB buffer extraction and isopropanol precipitation. Electrophoretic analysis showed fire type and smeared bands on all modifications. The modified Edwards et aL (1991) and Doyle and Doyle (1990) DNA extraction protocol were not successful in producing good quality DNA solution. <br/><br/> |
| 541 ## - IMMEDIATE SOURCE OF ACQUISITION NOTE |
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| Method of acquisition |
Submitted to the University Library |
| Date of acquisition |
05-09-2007 |
| Accession number |
T-3547 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Molecular biology |
| 9 (RLIN) |
1423 |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name entry element |
Genetics |
| General subdivision |
Plants |
| 9 (RLIN) |
926 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) |
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| 9 (RLIN) |
1672 |
| Course or Program |
Bachelor of Science in Biology |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| Personal name |
Ilagan, Yolanda A. |
| 9 (RLIN) |
22 |
| Relator term |
adviser |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| Electronic File |
<a href="http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=310aec6f16643fb93e2f5e6104fa05ca">http://library.cvsu.edu.ph/cgi-bin/koha/opac-retrieve-file.pl?id=310aec6f16643fb93e2f5e6104fa05ca</a> |
| Link text |
Click here to view thesis abstract and table of contents |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Theses / Manuscripts |
