Cytotoxic effect of Cycas zambaiensis using brine shrimp (Artemia salina) lethality biossay / by Ma. Janin D. Dionisio, Jenny Rose G. Galam and Ellaine B. Rodrin.
Material type:
TextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: qxiv, 72 pages : 28 cm. illustrations ; Content type: - text
- unmediated
- volume
- 610 D62 2015
- College of Nursing (CON)
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 610 D62 2015 (Browse shelf(Opens below)) | Link to resource | Room use only | T-6145 | 00010145 |
Thesis (Bachelor of Science in Medical Technology) Cavite State University
Includes bibliographical references.
College of Nursing (CON)
DIONISIO, MA. JANINE D., GALAM, JENNY ROSE G., AND RODRIN, ELLAINE B. Cytotoxic Effect of Cycas zambalensis using Brine Shrimp (Artemia salina) Lethality Bioassay. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite. October 2015. Adviser: Prof. Ronalyn Sanchez, RMT.
The study, entitled “Cytotoxic Effect of Cycas zambalensis using Brine Shrimp (Artemia salina) Lethality Bioassay,” was conducted at College of Nursing, Medical Technology Department, at Cavite State University, Indang, Cavite from April to October, 2014. Generally, the study aimed to assess the cytotoxic potential of Cycas zambalensis plant extracts using brine shrimp. Specifically, it aimed to: determine the phytochemical contents of the plant; assess which concentration of each extract has the greatest cytotoxic effect; compare which of the extracts is the most effective; and ascertain the lethal concentration of the extracts.
The phytochemical constituents of the leaves were qualitatively analyzed by submitting the samples to the Department of Science and Technology, Taguig City. Each of the ethanolic, methanolic and hexanolic extracts of Cycas zambalensis leaves were prepared in 10 000pg/ml, 1 000pg/ml, 100p¢/ml, and 10y¢g/ml concentrations and were screened for cytotoxicity potential using Brine Shrimp Lethality Assay (BSLA). Ten active nauplii in each vial were tested, with three replicates for each treatment. Positive and negative control was also tested. After 24 hours of incubation, the numbers of surviving shrimps were counted. Cytotoxicity was evaluated in terms of lethality concentration (LCs50).
Based on phytochemical analysis, Cycas zambalensis leaves contain flavonoids and tannins, which are known to have cytotoxic ability.
Ethanolic extracts showed a significant activity by the values of p<0.01 using one-way analysis of variance (ANOVA). Likewise, methanolic and hexanolic extracts showed a markedly significant cytotoxic activity by the values of p<0.05 using one-way analysis of variance (ANOVA).
Results of the study showed that all of the prepared extracts were found effective, however, hexanolic extract was the most potent against brine shrimps. The 1 000ug¢/ml concentration of ethanol and hexane extracts and 10 OO0Opg/ml concentration of methanolic extract of Cycas zambalensis showed the greatest cytotoxic effect against brine shrimps. Ethanolic, methanolic and hexanolic extract of Cycas zambalensis leaves were potent against brine shrimp with LCso values of 100, 100 and 10 pg/mL, respectively. Results were interpreted based on an LCso value of 1.0-10.0 indicates toxicity, and LCs9 >30 <100 pg/mL indicates mildly toxic.
Therefore, this may serve as baseline information for future researchers and may also be used as an alternative treatment or a medicinal plant to prevent the growth of cancer cells.
Submitted copy to the University Library. 02/14/2017 T-6145