Normal view MARC view ISBD view

Screening for anti-mitotic activity of protein fractions from ipil-ipil (Leucaena leucocephala) seeds / by Esteban M. Vasquez II.

By:

Vasquez, Esteban M. II [author]

Contributor(s):

Matel, Hosea dL [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xiii, 43 pages : 28 cm. illustrationsContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

547 V44 2016

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Arts and Science (CAS)

Abstract: VASQUEZ, ESTEBAN M. II. Screening for Antimitotic Activity of Protein Fractions from Ipil-Ipil (Leucaena leucocephala) Seeds. Undergraduate Thesis. | Bachelor of Science in Biology. Cavite State University. Indang, Cavite. April 2016. Adviser: Dr. Hosea dL. Matel. This study was conducted to: fractionate ipil-ipil seeds into albumin, globulin, glutelin and prolamin fractions; screen the different ipil-ipil seed protein fractions for anti-mitotic activity; and determine the morphological effects of ipil-ipil extracts to onion roots through Allium cepa test. In this study, mature ipil-ipil seeds were homogenized and subjected to Soxhlet extraction to remove lipid components using hexane. The remaining residue was fractionated through the Osborne method. Deionized water, 5 percent NaCl, 0.1M NaOH and 70 percent ethanol were used as extracting solvent to recover the albumin, globulin, glutelin and prolamin fractions, respectively. These protein fractions were subjected to anti-mitotic activity using Allium cepa test. Root tips were observed under the microscope and root growth and mitotic index were determined. Albumin and glutelin protein fractions significantly inhibited root growth and mitosis based on measured mitotic indices compared to the control. Morphological changes such as thinning, bending, spiralling and transparency were observed and existence of aberrant cells such as elongated, ballooned and ghost cells were also observed. There are protein components in the albumin and glutelin fractions of ipil-ipil seeds with anti-mitotic activity. Further cytotoxicity testing in human and cancer cells will help determine its therapeutic potential.

Average rating: 0.0 (0 votes)

Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	547 V44 2016 (Browse shelf(Opens below))		Link to resource	Room use only	T-6259		00010218

Thesis (Bachelor of Science in Biology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

VASQUEZ, ESTEBAN M. II. Screening for Antimitotic Activity of Protein Fractions from Ipil-Ipil (Leucaena leucocephala) Seeds. Undergraduate Thesis. | Bachelor of Science in Biology. Cavite State University. Indang, Cavite. April 2016. Adviser: Dr. Hosea dL. Matel.

This study was conducted to: fractionate ipil-ipil seeds into albumin, globulin, glutelin and prolamin fractions; screen the different ipil-ipil seed protein fractions for anti-mitotic activity; and determine the morphological effects of ipil-ipil extracts to onion roots through Allium cepa test.

In this study, mature ipil-ipil seeds were homogenized and subjected to Soxhlet extraction to remove lipid components using hexane. The remaining residue was fractionated through the Osborne method. Deionized water, 5 percent NaCl, 0.1M NaOH and 70 percent ethanol were used as extracting solvent to recover the albumin, globulin, glutelin and prolamin fractions, respectively. These protein fractions were subjected to anti-mitotic activity using Allium cepa test.

Root tips were observed under the microscope and root growth and mitotic index were determined. Albumin and glutelin protein fractions significantly inhibited root growth and mitosis based on measured mitotic indices compared to the control. Morphological changes such as thinning, bending, spiralling and transparency were observed and existence of aberrant cells such as elongated, ballooned and ghost cells were also observed.

There are protein components in the albumin and glutelin fractions of ipil-ipil seeds with anti-mitotic activity. Further cytotoxicity testing in human and cancer cells
will help determine its therapeutic potential.

Submitted copy to the University Library. 02/14/2017 T-6259