Microbilogical examination of air in medical department and incidence of nosocomial infection in selected secondary private hospitals in Cavite / by Erika Lei D. Colada and Alisa L. Jimenez.

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

COLADA, ERIKA LEI D. JIMENEZ, ALISA L. MICROBIOLOGICAL EXAMINATION OF AIR IN MEDICAL DEPARTMENT AND INCIDENCE OF NOSOCOMIAL INFECTION IN SELECTED SECONDARY PRIVATE
HOSPITALS IN CAVITE. Undergraduate Thesis. Bachelor of Science in Medical
Technology. Cavite State University Indang, Cavite. October 2016. Adviser: Karen Krista M. Escobar, RMT, MSMT
The collected samples for different medical department of selected secondary hospitals were processed at the microbiology laboratory of De La Salle Health Sciences Institute-Research Division Center for Basic Biomedical Research, City of Dasmarinas, Cavite. This study was conducted from July to September 2016. Generally, this study aimed to verify the microbiological examination of air in medical department and incidence of nosocomial infection in selected secondary private hospitals in Cavite. Specifically, it aimed to: (l) determine the demographic profile of each hospital in terms of: the year the hospital was established, the classification of medical department room — private or ward, the presence of air conditioning unit and the number of bed capacity; (2) determine the incidence of nosocomial infection from December 2015 to May 2016; (3) identify the isolated bacteria present in each medical ward and private room of secondary private hospitals in Cavite; (4) determine if there is a significant difference between the CFU result and the standard CFU for each hospital in each trial; and (5) determine the association between the bacteria isolated in each hospital and the incidence of nosocomial infection.
This study used descriptive design. There were two trials in each hospital, one in the medical ward and the other in a private room. Settle plate method was a passive air sampling used for testing quality of air in surgical operation theaters and hospital ward.
Based on the outcome of the study, the following conclusions were made: The morphological characteristics of Staphylococcus aureus exhibited small to large mucoid with creamy white, yellow and creamy white with ß-hemolysis colonies. The Staphylococcus epidermidis exhibited small to large cleanly white and yellow colonies. Pseudomonas aeruginosa showed rhizoid, irregular and rough colonies with indole negative (-), methyl red negative (-), voges proskauer (-), citrate (+) and TSI K/K. The isolated organisms showed positive results in Catalase test. Isolated nosocomial pathogens were Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The most frequent isolate was Staphylococcus aureus and Staphylococcus epidermidis. Based on the CFU result, there was no significant difference between the CFU result of the isolated bacteria and the standard CFU. There was no record of nosocomial infection in each hospital, therefore, the bacteria isolated in each hospital and the incidence of nosocomial infection had no association.
This study indicated that the nosocomial pathogens were frequently present in air of medical department. But the nosocomial pathogens were needed to exceed the acceptable level of index of microbial contamination to cause infection. To reduce the risk of nosocomial infections, this study recommended the following: Reorientation of the utility workers about alternative ways of cleaning procedures in medical departments specifically the proper ventilation of the rooms. The hospital should observe separating admitted infectious patients with non-infectious patients to avoid the spread of disease to others. Patients and health care personnel should be more aware, cautious, and careful of their surroundings specifically when there are possible pathogenic organisms present. It was also recommended to wear face mask; and hand washing must be implemented to avoid the spread of nosocomial infections. Further research study related to isolation of nosocomial bacterial pathogens in air of medical departments or other special areas of hospital should be conducted. The use of selective media to isolate different organisms and to easily identify different nosocomial pathogens present and isolation of nosocomial pathogens in air from different rooms and facilities (i.e., surgical rooms, emergency rooms, ICUs

Submitted copy to the University Library. 05/11/2017 T-6629