Effectiveness of Alugbati (Basella rubra) fruit extract as a histological stain / by Bless Marie B. Andaya and Abegail P. Topacio.

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

ANDAYA, BLESS MARIE B., ANO, CHRISTINE P. and TOPACIO, ABEGAIL P. Effectiveness of Alugbati (Basella Rubra) Fruit Extract as a Histological Stain. Undergraduate Thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. November 2016. Adviser: Ms. Karen Krista M. Escobar and Co-adviser: Mrs. Isolde M. Quitan.
Alugbati fruits used in the study were collected from Brgy. Bunga, Tanza, Cavite from July 2016. The chicken liver was obtained from Mahogany Market, Tagaytay City, Cavite, while the human tissue was obtained from Batangas Medical Center in Batangas City. The processing of tissues, extraction, staining, microscopic examination and recording of results were conducted at Cavite State University, Indang, Cavite from April to July 2016.
Generally, this study determined the effectiveness of alugbati (Basella rubra) fruit extract in staining tissue samples if it can be used as a histological stain. Specifically, this study determined the processes involved in the alugbati; determined the concentration of alugbati extracts which exhibits the best staining property in animal tissue particularly in chicken liver and human tissue particularly in human appendix; determined the types of cells which best exhibits strong pigment yield, clear nuclear detail and clear cytoplasmic detail; and determined which among the treatments exhibit the best staining property for all the cell types as compared to the hematoxylin stain.
The researchers used true experimental method where the results of the experimental groups are compared to the results of the control group not receiving the treatment. The alugbati fruits were extracted using a pounder and a strainer and then filtered using Whatman filter paper. The obtained alugbati fruit extract was added with distilled water where the treatments are made; Tl (100 mL alugbati fruit extract), T2 (75 mL alugbati fruit extract + 25 mL distilled water), T3 (50 mL alugbati fruit extract + 50 mL distilled water), T4 (25 mL alugbati fruit extract + 75 mL distilled water). The researchers stained the prepared slides of chicken and human tissue using commercial Hematoxylin stain for the positive control, and the treatments of alugbati fruit extract for the experimental group. After staining, tissue slides were subjected to microscopic observation.
Based on the concentration of alugbati fruit extract exhibiting best staining property in animal and human tissue, statistical values showed that only Tl (100mL pure alugbati fruit extract is comparable to the commercial Hematoxylin stain in terms of staining quality when stained in chicken liver tissue. On the other hand, all of the treatments are not comparable to the commercial Hematoxylin stain when stained in human appendix. The chicken liver yielded the highest statistical value in terms of strong pigment yield, clear nuclear detail and clear cytoplasmic detail compared to the human appendix. And based on the concentration of alugbati fruit extract exhibiting best staining property for all cell types, none of the treatments are comparable to the positive control in staining both animal and human tissue.
The researchers recommend the future researchers to find for a substance that will help give permanence of alugbati stain on tissues, utilize other methods of extracting alugbati fruits and consider factors such as pH and temperature in making a histological stain.

Submitted copy to the University Library. 05/02/2017 T-6603