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Alternative stain using bunga (Areca catechu) extract in diagnosing the cyst of Entamoeba histolytica and Entamoeba coli / by Rhodalyn M. Along, Jeline C. Ibaez, and Raizan B. Lazaro.

By:

Along, Rhodalyn M [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xv, 67 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

615 Al7 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Nursing (CON)

Abstract: ALONG, RHODALYN, IBANEZ, JELINE, LAZARO, RAIZAN, Alternative stain using Bunga (Areca catechu) Extract in Diagnosing the Cyst of Entamoeba histolytica and Entamoeba coli. Undergraduate Thesis of Bachelor of Science in Medical Technology Cavite State University, Indang, Cavite. April 2017, Adviser: Dr. Adelaida E. Sangalang, RMT This study aimed to determine the ability of Bunga (Areca catechu) extract in staining the cyst of Entamoeba histolytica and Entamoeba coli based on, pH level, nuclear and cytoplasmic detail, color stability and staining quality. The main purpose of the study is to determine the staining ability of Areca catechu (Bunga) extract in diagnosing the cyst of Entamoeba histolytica and Entamoeba coli against Lugol's iodine in wet mount preparation. Four treatments and a control replicated three times were used in the study, in five samples here allocated to each treatment. In three days the stained specimen was observe for its color stability and reagent strips was used for pH level identification. The researchers made a description to categorize the staining ability of every treatment used in the study. The study shows that Areca catechu extract used as an alternative stain to Lugol's iodine exhibit poor color stability, staining quality, nuclear and cytoplasmic detail. And it has poor staining ability against the cyst of Entamoeba histolytica and Entamoeba coli. All concentration of Bunga (Areca catechu) extracts was highly significant compared to the staining ability of Lugol's Iodine. The both control and treatment exhibit slightly acidic pH, In wet mount preparation Lugol 's iodine stained clearly to the cyst of Entamoeba hystolitica and coli in which the Bunga extract failed to demonstrate.

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Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	615 Al7 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-6624		00010906

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Previous	614.83 B33 2017 Analysis of potential health hazards among employees of registered hair salons in Cavite /	614.841 G13 2018 Preparedness of Cavite State University- Main Campus in fire incident /	615 Ag8 2016 Perception of senior citizens on generic drugs in selected areas of Cavite /	615 Al7 2017 Alternative stain using bunga (Areca catechu) extract in diagnosing the cyst of Entamoeba histolytica and Entamoeba coli /	615 An1 2015 Information dissemination on selected herbal medicine through mobile application /	615 An2 2016 Effectiveness of Alugbati (Basella rubra) fruit extract as a histological stain /	615 Ar2 2018 Antibacterial activity of Pandacaqui Puti (Tabernaemontana Pandacaqui Poir) plant parts extract against staphylococcus and Escherichia coli /	Next

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

ALONG, RHODALYN, IBANEZ, JELINE, LAZARO, RAIZAN, Alternative stain using Bunga (Areca catechu) Extract in Diagnosing the Cyst of Entamoeba histolytica and Entamoeba coli. Undergraduate Thesis of Bachelor of Science in Medical Technology Cavite State University, Indang, Cavite. April 2017, Adviser: Dr. Adelaida E. Sangalang, RMT

This study aimed to determine the ability of Bunga (Areca catechu) extract in staining the cyst of Entamoeba histolytica and Entamoeba coli based on, pH level, nuclear and cytoplasmic detail, color stability and staining quality. The main purpose of the study is to determine the staining ability of Areca catechu (Bunga) extract in diagnosing the cyst of Entamoeba histolytica and Entamoeba coli against Lugol's iodine in wet mount preparation.
Four treatments and a control replicated three times were used in the study, in five samples here allocated to each treatment. In three days the stained specimen was observe for its color stability and reagent strips was used for pH level identification. The researchers made a description to categorize the staining ability of every treatment used in the study.
The study shows that Areca catechu extract used as an alternative stain to Lugol's iodine exhibit poor color stability, staining quality, nuclear and cytoplasmic detail. And it has poor staining ability against the cyst of Entamoeba histolytica and Entamoeba coli.
All concentration of Bunga (Areca catechu) extracts was highly significant compared to the staining ability of Lugol's Iodine. The both control and treatment exhibit slightly acidic pH, In wet mount preparation Lugol 's iodine stained clearly to the cyst of Entamoeba hystolitica and coli in which the Bunga extract failed to demonstrate.

Submitted copy to the University Library. 05/10/2017 T-6624