Antimicrobial property of Bauhinia monandra (ALIBANGBANG) leaf extracts against selected pathogens / by Junee Elleigh C. Oway Yrhiz F. Oway and Lindsay Marie V. Reyes.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xiv, 76 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 615  Ow1 2015
Online resources: Production credits:
  • College of Nursing (CON)
Abstract: OWAY, JUNEE ELLEIGH C., OWAY, YRHIZ F., REYES, LINDSAY MARIE V. Antimicrobial Property of Bauhinia monandra (Alibangbang) Leaf Extracts Against Selected Pathogens. Undergraduate thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. October 2015. Adviser: Dr. Adelaida E. Sangalang. This study entitled "Antimicrobial Property of Bauhinia monandra (Alibangbang) Leaf Extracts Against Selected Pathogens" was conducted at the laboratory of the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. It generally aimed to determine the antimicrobial property of Bauhinia monandra against selected microorganisms. Specifically, it aimed to determine the constituents of Bauhinia monandra through phytochemical analysis; which plant extract would be more potent as an antimicrobial agent; and which pathogen would be most susceptible to the plant extract..The antimicrobial property of Bauhinia monandra was evaluated against Escherichia coli, Bacillus subtilis, Candida tropicalis and Fusarium oxysporum. Two extracting solvents were used in the study namely ethanol and hexane. The treatments were as follows: Tl used hexane extract; T2 used ethanol extract; T3 used positive control (ciprofloxacin for bacteria and fluconazole for fungi); lastly, T4 used the negative control (distilled water). For antibacterial assay, agar well diffusion technique was used in which the agar was suspended with each bacterial isolate. Each treatment was placed in four uniform wells cut from the agar. The zone of inhibition was measured after 24 hours of incubation at 370C. On the other hand, poisoned agar plate technique was used for antifungal assay. Each fungus was cultivated in the center of the plate, and incubated at 25 0C. The diameter of the fungal growth was measured after 14 days in which the negative control plate was completely colonized with mycelium. The study revealed that only the ethanol leaf extract of B. monandra exhibited inhibition of growth against Bacillus subtilis while hexane leaf extract showed no zone of inhibition. Both ethanol and hexane extracts of Bauhinia monandra against Escherichia coli showed no zone of inhibition as well. In antifungal activity, hexane and ethanol leaf extract against Fusarium oxysporum yields less antifungal activity as compared with the negative control. Moreover, only the ethanol leaf extract inhibited the growth Candida tropicalis completely whereas hexane was not as effective as ethanol. Based on the results obtained in the study, it was recommended to conduct further researches using the same plant involving the other parts of the plant to determine any antimicrobial property; to perform the antimicrobial assay using different concentrations of the plant extract which can be a factor affecting the result of the assay; and to make use of other extracting solvents to isolate the chemical constituents.
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Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

OWAY, JUNEE ELLEIGH C., OWAY, YRHIZ F., REYES, LINDSAY MARIE V. Antimicrobial Property of Bauhinia monandra (Alibangbang) Leaf
Extracts Against Selected Pathogens. Undergraduate thesis. Bachelor of Science in Medical Technology. Cavite State University, Indang, Cavite. October 2015. Adviser: Dr. Adelaida E. Sangalang.
This study entitled "Antimicrobial Property of Bauhinia monandra (Alibangbang)
Leaf Extracts Against Selected Pathogens" was conducted at the laboratory of the
Department of Medical Technology, College of Nursing, Cavite State University, Indang,
Cavite. It generally aimed to determine the antimicrobial property of Bauhinia monandra
against selected microorganisms. Specifically, it aimed to determine the constituents of
Bauhinia monandra through phytochemical analysis; which plant extract would be more potent as an antimicrobial agent; and which pathogen would be most susceptible to the
plant extract..The antimicrobial property of Bauhinia monandra was evaluated against
Escherichia coli, Bacillus subtilis, Candida tropicalis and Fusarium oxysporum. Two
extracting solvents were used in the study namely ethanol and hexane. The treatments
were as follows: Tl used hexane extract; T2 used ethanol extract; T3 used positive control
(ciprofloxacin for bacteria and fluconazole for fungi); lastly, T4 used the negative control
(distilled water). For antibacterial assay, agar well diffusion technique was used in which
the agar was suspended with each bacterial isolate. Each treatment was placed in four
uniform wells cut from the agar. The zone of inhibition was measured after 24 hours of incubation at 370C. On the other hand, poisoned agar plate technique was used for antifungal assay. Each fungus was cultivated in the center of the plate, and incubated at 25 0C. The diameter of the fungal growth was measured after 14 days in which the negative control plate was completely colonized with mycelium.
The study revealed that only the ethanol leaf extract of B. monandra exhibited inhibition of growth against Bacillus subtilis while hexane leaf extract showed no zone of inhibition. Both ethanol and hexane extracts of Bauhinia monandra against Escherichia coli showed no zone of inhibition as well. In antifungal activity, hexane and ethanol leaf extract against Fusarium oxysporum yields less antifungal activity as compared with the negative control. Moreover, only the ethanol leaf extract inhibited the growth Candida tropicalis completely whereas hexane was not as effective as ethanol.
Based on the results obtained in the study, it was recommended to conduct further researches using the same plant involving the other parts of the plant to determine any antimicrobial property; to perform the antimicrobial assay using different concentrations of the plant extract which can be a factor affecting the result of the assay; and to make use of other extracting solvents to isolate the chemical constituents.

Submitted copy to the University Library. 05/25/2017 T-6660

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