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Neutralizing effect of caffeine (1,2,3, -- Trimethyl Xanthine) against Philippine cobra venom (Naja philippinensis) / by Quennie Rose P. Barros and Rhiandee A. Jabol.

By:

Barrosl, Quennie Rose P [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xiii, 57 p. : col. ill. ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

597.96 B27 2015

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Nursing (CON)

Abstract: BARROS, QUENNIE ROSE P., AND JABOL, RHIANDEE A. Neutralizing Effect Of Caffeine (1,3,7-trimethylxanthine) Against Philippine Cobra Venom (Naja Philippines’s).Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, October 2015. Adviser: Ms. Ronalyn S. Sanchez. This study was conducted at the Medical Technology Laboratory, Cavite State University, Indang, Cavite from January to June 2015. It aimed to determine the neutralizing effects of caffeine (1,3,7-trimethylxanthine) against Philippine cobra venom (Naja Philippines’s). Phospholipase enzyme activity of the cobra venom was determined using Egg hydrolysis test developed by Yap, Tan, &Fung, (2011). Different concentration of caffeine namely 308gM,103gM, 64.57gM, & 25.7 YIM were added to the egg yolk venom solution. Changes in pH level in the solution were then recorded after one hour of incubation. Therapeutic dose 50 of caffeine, concentrations 308gM, 103gM, 64.57gM, & 25.7 uM, were tested on venom injected earthworm (Eudrilus euginae) (Wang, et al; 2011). Number of live earthworms were then counted after 24 hours of incubation. The pH of the egg yolk solution dropped turning the solution acidic. However, the downward shift was abated upon treatments of caffeine concentration. This downward shift was due to the hydrolyzing effect of phospholipase enzyme present on the cobra venom at —acyl ester bonds of 3-sn-phospholipids yielding fatty acids and Lys phospholipids (Sam, Gopalakrishnakone, & Chow, 2012). Analysis of variance has showed trial I was significant at p-value of 0.004 having 103 UM caffeine concentration as the significant treatment using Tukey analysis. Probit analysis of the therapeutic dose 50 on venom-treated earthworm have shown that 103 YM of caffeine was significant on neutralizing the venom activity as compared to other treatments.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	597.96 B27 2015 (Browse shelf(Opens below))		Link to resource	Room use only	T-6657		00010915

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

BARROS, QUENNIE ROSE P., AND JABOL, RHIANDEE A. Neutralizing Effect Of Caffeine (1,3,7-trimethylxanthine) Against Philippine Cobra Venom (Naja Philippines’s).Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, October 2015. Adviser: Ms. Ronalyn S. Sanchez.
This study was conducted at the Medical Technology Laboratory, Cavite State University, Indang, Cavite from January to June 2015. It aimed to determine the neutralizing effects of caffeine (1,3,7-trimethylxanthine) against Philippine cobra venom (Naja Philippines’s).
Phospholipase enzyme activity of the cobra venom was determined using Egg hydrolysis test developed by Yap, Tan, &Fung, (2011). Different concentration of caffeine namely 308gM,103gM, 64.57gM, & 25.7 YIM were added to the egg yolk venom solution. Changes in pH level in the solution were then recorded after one hour of incubation. Therapeutic dose 50 of caffeine, concentrations 308gM, 103gM, 64.57gM, & 25.7 uM, were tested on venom injected earthworm (Eudrilus euginae) (Wang, et al; 2011). Number of live earthworms were then counted after 24 hours of incubation.
The pH of the egg yolk solution dropped turning the solution acidic. However, the downward shift was abated upon treatments of caffeine concentration. This downward shift was due to the hydrolyzing effect of phospholipase enzyme present on the cobra venom at —acyl ester bonds of 3-sn-phospholipids yielding fatty acids and Lys phospholipids (Sam, Gopalakrishnakone, & Chow, 2012). Analysis of variance has showed trial I was significant at p-value of 0.004 having 103 UM caffeine concentration as the significant treatment using Tukey analysis. Probit analysis of the therapeutic dose 50 on venom-treated earthworm have shown that 103 YM of caffeine was significant on neutralizing the venom activity as compared to other treatments.

Submitted copy to the University Library. 05/25/2017 T-6657