Antibiogram of enterohemorrhagic Escherichia coli (EHEC) in the faces of native chickens (Gallus-gallus L.) from selected farms in upland Cavite, Phi / by Jonathan Osis Chua.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xvi, 97 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.5  C47 2016
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: CHUA, JONATHAN 0. Antibiogram of Enterohemorrhagic Escherichia coli (EHEC) in the Feces of Native Chickens (Gallus gallus L.) from Selected Farms in Upland Cavite, Philippines. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2016. Adviser: Dr. Ma. Cynthia R. dela Cruz. The purpose of this study is to isolate enterohemorrhagic Escherichiacoil (EHEC) in the fecal samples of native chickens and determine its antibacterial susceptibility profile. One hundred fecal samples from native chickens from selected farms in upland, Cavite were used to determine the prevalence of EHEC in the area. Fecal samples from cloacal swabs were initially placed in a sterile Brain-Heart Infusion Broth (BHI). Fourteen samples have shown clear, pale colored colonies when grown in Sorbitol Mac Conkey Agar (SMAC) characterizing presumptive Enterohemorrhagic Escherichia coll. There were a total of 8 E. coil organisms identified through biochemical methods. Among the 8 presumptive Escherichta colt isolates which generated negative for so bitol oxidation and fermentation, only one isolate generated positive result for hemolysis test and therefore considered to be pathogenic, having a hemolytic prevalence of 1% (1/100). The antibiotic susceptibility test of the isolated organisms is found to be 100% susceptible to Enrofloxacin, 62.5% to gentamicin, 50% to Trimethoprim-sulfamethoxazole and 12.5% to spectinomycin. On the other hand, the isolates were 100% resistant to Bacitracin, Erythromycin, Neomycin and Penicillin G based on the standard set by CLSI. This study showed that multidrug resistant EHEC isolates were found in native chickens and can be a source of infection to other animals and humans.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.5 C47 2016 (Browse shelf(Opens below)) Link to resource Room use only T-6160 00011015

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

CHUA, JONATHAN 0. Antibiogram of Enterohemorrhagic Escherichia coli (EHEC) in the Feces of Native Chickens (Gallus gallus L.) from Selected Farms in Upland Cavite, Philippines. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2016. Adviser: Dr. Ma. Cynthia R. dela Cruz.

The purpose of this study is to isolate enterohemorrhagic Escherichiacoil (EHEC) in the fecal samples of native chickens and determine its antibacterial susceptibility profile. One hundred fecal samples from native chickens from selected farms in upland, Cavite were used to determine the prevalence of EHEC in the area. Fecal samples from cloacal swabs were initially placed in a sterile Brain-Heart Infusion Broth (BHI). Fourteen samples have shown clear, pale colored colonies when grown in Sorbitol Mac Conkey Agar (SMAC) characterizing presumptive Enterohemorrhagic Escherichia coll. There were a total of 8 E. coil organisms identified through biochemical methods. Among the 8 presumptive Escherichta colt isolates which generated negative for so bitol oxidation and fermentation, only one isolate generated positive result for hemolysis test and therefore considered to be pathogenic, having a hemolytic prevalence of 1% (1/100). The antibiotic susceptibility test of the isolated organisms is found to be 100% susceptible to Enrofloxacin, 62.5% to gentamicin, 50% to Trimethoprim-sulfamethoxazole and 12.5% to spectinomycin. On the other hand, the isolates were 100% resistant to Bacitracin, Erythromycin, Neomycin and Penicillin G based on the standard set by CLSI. This study showed that multidrug resistant EHEC isolates were found in native chickens and can be a source of infection to other animals and humans.

Submitted copy to the University Library. 02-14-2017 T-6160

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