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Detection of mt-rs s gene and flavivirus in ticks from cattle in the province of Cavite using polymerase chain reaction / by Mylord D. Dimasacat.

By:

Dimasacat, Mylord D [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xiii, 59 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

595.4 D59 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biomedical Sciences (VETMET)

Abstract: DIMASACAT, MYLORD DIMAANO., Detection of mt-rrs gene and Flavivirus in ticks from cattle in the province of Cavite using Polymerase Chain Reaction Cavite Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2017. Adviser: Dr. Cherry R Alvarez. The study was conducted to detect the presence of mt-rrs gene and Flavivirus from the cattle in the selected farms of the central hilly and lowland areas in the province of Cavite. Manual picking of ticks was performed in the cattle's body surface while flagging and dragging methods were employed in the pasture land. A total of one hundred (100) ticks were selected and used for the DNA/RNA extraction. The cDNA was synthesized using the ReverTra Ace-u-@ and were initially tested for the presence of mtrrs gene through the conventional Polymerase Chain Reaction (PCR). Of the eighty-nine (89) remaining samples, eighty (80) samples were positive for mt-rrs gene. This is comprised of eight (8) adult male ticks and seventy-two (72) adult feinale ticks, with a detection rate of 88.88% and 90.00%, respectively. All the mt-rrs positive samples were subjected to the detection of Flavivirus using Langat virus as positive control through conventional PCR but no gene was amplified. The results of this study suggested that the tick-borne Flaviviruses may not be present in the areas where the samples were collected. The author recommends that further study be conducted with a higher sample size, with nymph and larval stages of ticks as samples may also be included. Furthermore, pooling of samples may be considered to increase the possibility of detecting the pathogen by the number of ticks per pool. Lastly, inclusion of other xii animals, small ruminants, for instance, may be considered as sample animals

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	595.4 D59 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-6818		00011227

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

DIMASACAT, MYLORD DIMAANO., Detection of mt-rrs gene and Flavivirus in ticks from cattle in the province of Cavite using Polymerase Chain Reaction Cavite Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2017. Adviser: Dr. Cherry R Alvarez.

The study was conducted to detect the presence of mt-rrs gene and Flavivirus from the cattle in the selected farms of the central hilly and lowland areas in the province of Cavite. Manual picking of ticks was performed in the cattle's body surface while flagging and dragging methods were employed in the pasture land. A total of one hundred (100) ticks were selected and used for the DNA/RNA extraction. The cDNA was synthesized using the ReverTra Ace-u-@ and were initially tested for the presence of mtrrs gene through the conventional Polymerase Chain Reaction (PCR).
Of the eighty-nine (89) remaining samples, eighty (80) samples were positive for mt-rrs gene. This is comprised of eight (8) adult male ticks and seventy-two (72) adult feinale ticks, with a detection rate of 88.88% and 90.00%, respectively.
All the mt-rrs positive samples were subjected to the detection of Flavivirus using Langat virus as positive control through conventional PCR but no gene was amplified. The results of this study suggested that the tick-borne Flaviviruses may not be present in the areas where the samples were collected.
The author recommends that further study be conducted with a higher sample size, with nymph and larval stages of ticks as samples may also be included. Furthermore, pooling of samples may be considered to increase the possibility of detecting the pathogen by the number of ticks per pool. Lastly, inclusion of other
xii
animals, small ruminants, for instance, may be considered as sample animals

Submitted copy to the University Library. 08/01/2017 T-6818