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Detection of mt-rss gene and Coxiella burnetii among ticks collected from cattle and pasture in Cavite using polymerase chain reaction / by Florante Homoroc Generoso.

By:

Generoso, Florante Homoroc [author]

Contributor(s):

Encarnacion, Noemi D [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xivl, 62 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

595.4 G28 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biomedical Sciences (VETMET)

Abstract: FLORANTE H. GENEROSO, DETECTION OF mt-rrs GENE and Coxiella burnetii AMONG TICKS COLLECTED FROM CATTLE AND PASTURE IN CAVITE USING POLYMERASE CHAIN REACTION, Undergraduate Thesis. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite, May 2017, Adviser: Noemi D. Encarnacion, DVM, MVetEpi Ticks are considered vectors of pathogens for animals and humans. They are obligate ectoparasites that feed on blood tissues of wide host range. This study aimed to determine the presence of mt-rrs gene and Coxiella burnetti among tick samples from cattle farms within the province of Cavite, Philippines using polymerase chain reaction. A total 294 tick samples, 63 from lowland (21.42%) and 231 from central hilly (78.57%) were used in this study. All are of Rhipicephalus microplus species and comprises of 293 adults (99.65%) and I larva (0.35%). Ticks among the adult stage consist of 18 males (6.14%) and 275 females (93.85%). Out of 294 samples, 205 tick DNA extracted using the boiling method detected 85 mt-rrs gene while using the BlackPREP Tick DNA/RNA kit (Life Science, Germany), out of the 89 tick DNA extracted, mt-rrs was detected in 80 samples. One hundred sixtyfive (165) tick DNA samples were ran for pathogen detection using Coxiel/a-specific primers. Zero (0) out of 165 tick DNA samples tested positive for the presence of Coxiella burnetii. One (1) sample on the other hand, produced a single, clear, but having a smaller amplicon suggesting the presence of Coxie//a-like endosymbiont in the tick POPLIIation. However, phylogenetic analysis is needed to support such claim. This study documents the detection rate of the mt-rrs gene using the boiling method and BlackPREP Tick DNA/RNA kit, (Life Science, Germany); and preliminary molecular survey of Coxiella burnetii from cattle ticks in Cavite.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	595.4 G28 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-6819		00011231

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

FLORANTE H. GENEROSO, DETECTION OF mt-rrs GENE and Coxiella burnetii AMONG TICKS COLLECTED FROM CATTLE AND PASTURE IN CAVITE USING POLYMERASE CHAIN REACTION, Undergraduate Thesis. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite, May 2017, Adviser: Noemi D. Encarnacion, DVM, MVetEpi
Ticks are considered vectors of pathogens for animals and humans. They are
obligate ectoparasites that feed on blood tissues of wide host range. This study aimed to determine the presence of mt-rrs gene and Coxiella burnetti among tick samples from cattle farms within the province of Cavite, Philippines using polymerase chain reaction.
A total 294 tick samples, 63 from lowland (21.42%) and 231 from central hilly (78.57%) were used in this study. All are of Rhipicephalus microplus species and comprises of 293 adults (99.65%) and I larva (0.35%). Ticks among the adult stage
consist of 18 males (6.14%) and 275 females (93.85%).
Out of 294 samples, 205 tick DNA extracted using the boiling method detected 85
mt-rrs gene while using the BlackPREP Tick DNA/RNA kit (Life Science, Germany),
out of the 89 tick DNA extracted, mt-rrs was detected in 80 samples. One hundred sixtyfive (165) tick DNA samples were ran for pathogen detection using Coxiel/a-specific
primers. Zero (0) out of 165 tick DNA samples tested positive for the presence of Coxiella burnetii. One (1) sample on the other hand, produced a single, clear, but having
a smaller amplicon suggesting the presence of Coxie//a-like endosymbiont in the tick
POPLIIation. However, phylogenetic analysis is needed to support such claim.
This study documents the detection rate of the mt-rrs gene using the boiling method and BlackPREP Tick DNA/RNA kit, (Life Science, Germany); and preliminary molecular survey of Coxiella burnetii from cattle ticks in Cavite.

Submitted copy to the University Library. 08/01/2017 T-6819