Fungal communities associated with adult stingless bees, honey and pollen provisions of Ttetragonula biroi Friese / by John Robert R. Pelobello.

By:

Pelobello, John Robert R [author]

Contributor(s):

Bono, Michele T [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2017.Description: xv, 97 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

595.79 P36 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Arts and Science (CAS)

Abstract: PELOBELLO, JOHN ROBERT ROSALES, April 2017. "Fungal Communities Associated with Adult Stingless Bees, Honey, And Pollen Provisions Of (Tetragonula Biroi Friese)".An Undergraduate thesis Bachelor of Science in Biology, Cavite State University, Indang, Cavite, Adviser: Prof. Michele T. Bono. This study was conducted to: determine the physico-chemical characteristics of honey, pollen and the stingless bee hives; determine the fungal count of honey and pollen provisions on the three selected sites in Indang, Cavite; isolate and identify the fungi isolated from pollen provisions, honey, and adult stingless bees; compare the fungi isolated from honey, pollen and adult stingless bees from different sites of Indang, Cavite; and determine the relationship of the isolated fungi and the physico-chemical properties of the honey and pollen. The results of the different physico-chemical parameters showed that T. biroi honey varies as follows: pH (3.70 -4.20), moisture content (36.08-50.6%), and sugar content (67.95 - 73.15 0B). Pollen physicochemical parameters also vary as follows: pH (3 67 -4.18) and moisture content (28.45-41.36%). The temperature inside the stingless bee hive is at 25.55-29.75 0C and the relative humidity is at 62-87.5%. In honey, yeast counts range from 3.73 — 21.67 x 102 CFU/ml and mold counts ranges from 0.25-4.23 x 102 CFU/ml. In pollen, yeasts counts ranges from 4.46 - 21.27 CFU/g and molds counts ranges from 0.46 10.23 CFU/g. The highest mold count is observed in Alulod (10 23 x 102 CFU/g) while the lowest at Daine (0.25 x 102 CFU/g). The highest yeast count is observed in Alulod (21.67 x 102 CFU/ml) while the lowest counts at Daine (3 73 x 102 CFU/ml). Isolated fungi are under the genera Aspergillus, Penicillium, Mucor, Cladosporium, Candida, Kloeckera, and Saccharomyces. Among these, Aspergillus, Penicillium, Candida, and Saccharomyces were most dominantly found on honey, pollen and adult bees.The pH of the honey and pollen are highly correlated with the both yeast and mold counts. Moisture content, hive temperature and hive humidity are highly correlated with the yeast counts on pollen. Mold counts are highly correlated with the pH and moisture content of both honey and pollen samples.

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Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	595.79 P36 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-6873		00011237

Thesis (BS Biology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

PELOBELLO, JOHN ROBERT ROSALES, April 2017. "Fungal Communities Associated with Adult Stingless Bees, Honey, And Pollen Provisions Of (Tetragonula Biroi Friese)".An Undergraduate thesis Bachelor of Science in Biology, Cavite State University, Indang, Cavite, Adviser: Prof. Michele T. Bono.

This study was conducted to: determine the physico-chemical characteristics of honey, pollen and the stingless bee hives; determine the fungal count of honey and pollen provisions on the three selected sites in Indang, Cavite; isolate and identify the fungi isolated from pollen provisions, honey, and adult stingless bees; compare the fungi isolated from honey, pollen and adult stingless bees from different sites of Indang, Cavite; and determine the relationship of the isolated fungi and the physico-chemical properties of the honey and pollen.
The results of the different physico-chemical parameters showed that T. biroi honey varies as follows: pH (3.70 -4.20), moisture content (36.08-50.6%), and sugar content (67.95 - 73.15 0B). Pollen physicochemical parameters also vary as follows: pH (3 67 -4.18) and moisture content (28.45-41.36%). The temperature inside the stingless bee hive is at 25.55-29.75 0C and the relative humidity is at 62-87.5%.
In honey, yeast counts range from 3.73 — 21.67 x 102 CFU/ml and mold counts ranges from 0.25-4.23 x 102 CFU/ml. In pollen, yeasts counts ranges from 4.46 - 21.27 CFU/g and molds counts ranges from 0.46 10.23 CFU/g.
The highest mold count is observed in Alulod (10 23 x 102 CFU/g) while the lowest at Daine (0.25 x 102 CFU/g). The highest yeast count is observed in Alulod (21.67 x 102 CFU/ml) while the lowest counts at Daine (3 73 x 102 CFU/ml).
Isolated fungi are under the genera Aspergillus, Penicillium, Mucor, Cladosporium, Candida, Kloeckera, and Saccharomyces. Among these, Aspergillus, Penicillium, Candida, and Saccharomyces were most dominantly found on honey, pollen and adult bees.The pH of the honey and pollen are highly correlated with the both yeast and mold counts. Moisture content, hive temperature and hive humidity are highly correlated with the yeast counts on pollen. Mold counts are highly correlated with the pH and moisture content of both honey and pollen samples.

Submitted copy to the University Library. 08/03/2017 T-6873

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