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Embryo and tissue culture of Strelitzia reginae Ait. / Kongsakdi Promtep.

By:

Promtep, Kongsakdi [author]

Contributor(s):

Valmeyor, Helen L [adviser]

Material type: Text

TextLanguage: English Publication details: Los Baños, Laguna : University of the Philippines- Los Baños, 1981.Description: 196 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

581.8 P94 1981

Online resources:

Click here to view thesis abstract and table of contents

Abstract: KONGSAKDI PROMTEP, University of the Philippines at Los Banos, July 1981. Embryo and Tissue Culture of Strelitzia Reginae Ait. Major Professor: Dr. Helen L. Valmeyor,. Two sets of experiments were conducted to explore the potential of in vitro method for propagating the excised embryo and shoot tip of Strelitzia reginae. The excised embryo germinate readily in Whitets medium without vitamins in 7 days. Compared to other basal media tested, highly modified Murashige and Skoog's medium (MS) enhanced the growth of seedlings. With MS bosal media, the growth of seedlings from embryos wes accelerated when treated with a combination of NAA and coconut water. There was synergistic effect of these two substances in terms of enhanced growth of shoot and fibrous root formation, Ki enhanced the growth of shoot and root. Although BA also enhanced shoot growth, root elongation was inhibited, Callus formation from shoot tip was induced by Sequential culture in media with a combination of BA and NAA, followed by modified SH medium, Three types of calluses were found: friable, water-soaked and surface calluses. All types, however, failed to show sustained growth and differentiation. The increase in shoot number sometimes occurred in cytokinin treatments. Combination of Ki and 2,4-D or modified SH and MS medium induced bud formation, but at a low frequency. Half shoot tips could be used successfully for the in vitro multiplication of &- Eeginee. Results showed that embryo and tissue culture could be more promising than the other conventional methods of multiplying S. reginae. Embryo culture could induce faster growth and higher germination rate. The limited success in callus induction and by proliferation also indicate the possibility of developing @ better method of clonal propagation and mass production of this kind of plant in the future.

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Holdings ( 1 )
Title notes ( 4 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	581.8 P94 1981 (Browse shelf(Opens below))		Link to resource	Room use only	T-1492		00001164

Thesis (Ph.D. - - Horticulture) University of the Philippines, College, Laguna.

Includes bibliographical references.

KONGSAKDI PROMTEP, University of the Philippines at Los Banos, July 1981. Embryo and Tissue Culture of Strelitzia Reginae Ait. Major Professor: Dr. Helen L. Valmeyor,.

Two sets of experiments were conducted to explore the potential of in vitro method for propagating the excised embryo and shoot tip of Strelitzia reginae.

The excised embryo germinate readily in Whitets medium without vitamins in 7 days. Compared to other basal media tested, highly modified Murashige and Skoog's medium (MS) enhanced the growth of seedlings. With MS bosal media, the growth of seedlings from embryos wes accelerated when treated with a combination of NAA and coconut water. There was synergistic effect of these two substances in terms of enhanced growth of shoot and fibrous root formation,

Ki enhanced the growth of shoot and root. Although BA also enhanced shoot growth, root elongation was inhibited, Callus formation from shoot tip was induced by Sequential culture in media with a combination of BA and NAA, followed by modified SH medium, Three types of calluses were found: friable, water-soaked and surface calluses. All types, however, failed to show sustained growth and differentiation.

The increase in shoot number sometimes occurred in cytokinin treatments. Combination of Ki and 2,4-D or modified SH and MS medium induced bud formation, but at a low frequency. Half shoot tips could be used successfully for the in vitro multiplication of &- Eeginee.

Results showed that embryo and tissue culture could be more promising than the other conventional methods of multiplying S. reginae. Embryo culture could induce faster growth and higher germination rate.

The limited success in callus induction and by proliferation also indicate the possibility of developing @ better method of clonal propagation and mass production of this kind of plant in the future.

Submitted to the University Library 01/07/1994 T-1492