Detection of subclinical mastitis in dairy buffaloes (babalus bubalis) from selected farms from Cavite / by Kim Casey Dahilog Solitario.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xii, 51 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
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  • volume
Subject(s): DDC classification:
  • 636.2142  So4 2017
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: SOLITARIO, KIM CASEY D. Detection of subclinical mastitis in dairy buffaloes (Bubalus bubalis) in selected farms in Cavite. Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. July 2017. Adviser: Chester Joshua V. Saldafia, DVM, MSc. A study was conducted to detect the presence of subclinical mastitis in raw milk of dairy buffaloes in Cavite. A total of 59 milk samples from dairy buffalo backyard farms in General Trias, Cavite were enrolled in the study. Milk samples were collected and processed immediately for California Mastitis Test. Further tests were conducted in the laboratory to determine the Somatic Cell Count (SCC) using Fossomatic™ FC and the Total Viable Count (TVC) of milk samples by serially diluting the milk up to 10’ using normal saline solution as diluent, plated onto Nutrient Agar plates and incubated at various temperatures. Results of the study revealed that 15.25% (9/59) of the total dairy buffaloes were positive for subclinical mastitis. Seven out of 20 (35%) samples from Brgy. Pasong Camichille I and Two out of 14 (14%) samples from Brgy. Santiago were positive for subclinical mastitis. The Total Viable Count (TVC) of fresh buffalo milks ranged from 1.12 x 10° to 2.79 x 10°. The mean viable count at 37°C incubation is 7.5 x 10° cfu/mL. This is higher compared with the viable counts of 4.7 x 10° cfu/mL at 25 °C although no statistical difference was found. No growth was observed at 5 °C and 59 °C. Furthermore, the presence of subclinical mastitis was statistically associated with the total viable count. The somatic cell count (SCC) of fresh buffalo milks from sample farms in Cavite ranges from 1.4 x 10* to 8.8 x 10° with a mean of 1.5 x 10° cells/ml. Moreover, the Total . Viable Count (TVC) and Somatic Cell Count (SCC) have sensitivity of 89% and 100% and specificity of 96% and 100% respectively. This means that if mastitis is present, there is an 89% chance that TVC and 100% chance that SCC will detect it. The Somatic Cell Count (SCC) is a dependable test to diagnose subclinical mastitis in buffaloes like the California Mastitis Test (CMT) that are commonly used in dairy farms. This study revealed that the subclinical mastitis is prevalent in milking buffaloes in Cavite.
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636.214 V23 1980 The effects of two dietary levels of protein and two systems of housing management on the growth and performance of dairy calves / 636.2142 Sa1 2007 Isolation, characterization and antibiotic sensitivity testing of hemolysin-producing Escherichia coli 0157 : H7 in carrier dairy cattle of the Dairy Trainig and Research Institute (DIRI) Laguna, Philippines / 636.2142 So4 2009 Dairy cattle raising practices at Dairy Training and Research Institute (DTRI) University of the Philippines, Los Baños, College Laguna : a farm practice / 636.2142 So4 2017 Detection of subclinical mastitis in dairy buffaloes (babalus bubalis) from selected farms from Cavite / 636.2143 C11 2017 Detection of subclinical mastitis in dairy goats (capra hircus) in selected farms from Cavite and Batangas / 636.293 Ar1 2003 A preliminary study on the milk quality of bulgarian murrah buffalo (BMB) from the Philippine Carabao Center (PCC) cooperators in selected towns of Cavite 636.293 C88 1982 Farmers' perceptions and attitudes toward carabao breeding practices and the reproductive performance of carabaos bred by artificial insemination or natural mating under farm conditions

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

SOLITARIO, KIM CASEY D. Detection of subclinical mastitis in dairy buffaloes (Bubalus bubalis) in selected farms in Cavite. Undergraduate Thesis, Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. July 2017. Adviser: Chester Joshua V. Saldafia, DVM, MSc.

A study was conducted to detect the presence of subclinical mastitis in raw milk of dairy buffaloes in Cavite. A total of 59 milk samples from dairy buffalo backyard farms in General Trias, Cavite were enrolled in the study. Milk samples were collected and processed immediately for California Mastitis Test. Further tests were conducted in the laboratory to determine the Somatic Cell Count (SCC) using Fossomatic™ FC and the Total Viable Count (TVC) of milk samples by serially diluting the milk up to 10’ using normal saline solution as diluent, plated onto Nutrient Agar plates and incubated at various
temperatures.

Results of the study revealed that 15.25% (9/59) of the total dairy buffaloes were positive for subclinical mastitis. Seven out of 20 (35%) samples from Brgy. Pasong Camichille I and Two out of 14 (14%) samples from Brgy. Santiago were positive for subclinical mastitis. The Total Viable Count (TVC) of fresh buffalo milks ranged from
1.12 x 10° to 2.79 x 10°. The mean viable count at 37°C incubation is 7.5 x 10° cfu/mL. This is higher compared with the viable counts of 4.7 x 10° cfu/mL at 25 °C although no statistical difference was found. No growth was observed at 5 °C and 59 °C. Furthermore, the presence of subclinical mastitis was statistically associated with the total viable count.

The somatic cell count (SCC) of fresh buffalo milks from sample farms in Cavite ranges from 1.4 x 10* to 8.8 x 10° with a mean of 1.5 x 10° cells/ml. Moreover, the Total . Viable Count (TVC) and Somatic Cell Count (SCC) have sensitivity of 89% and 100% and specificity of 96% and 100% respectively. This means that if mastitis is present, there is an 89% chance that TVC and 100% chance that SCC will detect it. The Somatic Cell Count (SCC) is a dependable test to diagnose subclinical mastitis in buffaloes like the California Mastitis Test (CMT) that are commonly used in dairy farms.

This study revealed that the subclinical mastitis is prevalent in milking buffaloes in Cavite.

Submitted copy to the University Library. 09/20/2017 T-7097

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