Skip-reflasking in the embryo culture of vanda (V. sanderana) using different growing media / by Nilda D. Arandia

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Don Severino Agricultural College, 1988.Description: 27 pages: illustrations ; 27 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 635.9 Ar1 1988
Online resources: Production credits:
  • Science High School, College of Education (CED)
Abstract: ARANDIA, NILDA D., Applied Research IV (Agricultural Science Curriculum), Don Severino Agricultural College, Indang, Cavite, March 1988. SKIP-REFLASKING IN THE EMBRYO CULTURE OF VANDA (V. sanderana) USING DIFFERENT GROWING MEDIA. Adviser: Mr. Alejandro C. Mojica The experiment was conducted to discover the effect of skip-reflasking and different growing media for Vanda sanderana. Reflasking (control) was done by using Knudson ¢ as growing medium; skip-reflasking; Treatment 2, by using Coconut husk; Treatment 3 by using Sphagnum moss; Treatment 4 by using Osmunda fiber; Treatment 5 by using Paslak; and Treatment 6 by using Charcoal. The experiment revealed that in embryo culture of Vanda sanderana, reflasking protocorms is necessary as the sécond step in the culture. Seedlings develop inside the bottles are more vigorous that they grow and develop faster compared to seedlings grown from other media. Moreover, on skip-reflasking, Paslak as growing media is good enough as a source of planting material. It was followed by seedlings grown from Charcoal and Coconut husk, the growing media. Seedlings grown from Sphagnum moss and Osmunda fiber show poor growth.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 635.9 Ar1 1988 (Browse shelf(Opens below)) Link to resource Room use only R-39 00000277

Applied Research IV (Agri-Science Curriculum) Don Severino Agricultural College.

Includes bibliographical references.

Science High School, College of Education (CED)

ARANDIA, NILDA D., Applied Research IV (Agricultural Science Curriculum), Don Severino Agricultural College, Indang, Cavite, March 1988. SKIP-REFLASKING IN THE EMBRYO CULTURE OF VANDA (V. sanderana) USING DIFFERENT GROWING MEDIA. Adviser: Mr. Alejandro C. Mojica

The experiment was conducted to discover the effect of skip-reflasking and different growing media for Vanda sanderana.

Reflasking (control) was done by using Knudson ¢ as growing medium; skip-reflasking; Treatment 2, by using Coconut husk; Treatment 3 by using Sphagnum moss; Treatment 4 by using Osmunda fiber; Treatment 5 by using Paslak; and Treatment 6 by using Charcoal.

The experiment revealed that in embryo culture of Vanda sanderana, reflasking protocorms is necessary as the sécond step in the culture. Seedlings develop inside the bottles are more vigorous that they grow and develop faster compared to seedlings grown from other media.

Moreover, on skip-reflasking, Paslak as growing media is good enough as a source of planting material. It was followed by seedlings grown from Charcoal and Coconut husk, the growing media. Seedlings grown from Sphagnum moss and Osmunda fiber show poor growth.


Submitted to the University Library R-39

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