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Enhancement of low-diversity polyethylene (LDPE) plastic biodegration through fungal consortium / by Jan Cleomarie C. Cenizal.

By:

Cenizal, Jan Cleomarie C [author]

Contributor(s):

Cruzate, Sherine M [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xiii, 90 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

668.4 C32 2019

Online resources:

View Abstract and table of contents

Production credits:

College of Arts and Sciences (CAS), Department of Biological Sciences

Abstract: CENIZAL, JAN CLEOMARIE C. "Enhancement of Low Density Polyethylene (LDPE) Plastic Biodegradation through Fungal Consortium". Undergraduate Thesis. Bachelor of Science in Biology, major in Microbiology. Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Indang, Cavite. May 2019. Adviser: Prof. Sherine M. Cruzate. This study focused on the enhancement of the biodegradation of LDPE plastic using the locally isolated Aspergillus niger, Paecilotnyces spp. and Penicillium spp., as fungal consortium. Compatibility test was done as initial test if these fungi could grow together. Thereafter, they were subjected to in vitro biodegradation assay where the effect of different parameters such as plastic size, carbon sources, nitrogen sources, pH, and agitation on the growth and degradation of fungal consortium were determined after 30 days of incubation. The most promising fungal consortium was the combination of Aspergillus niger and Penicillium spp. (ASPE). In vitro biodegradation assay revealed that this fungal consortium had high biomass when grown on Mineral Salt Broth (MSB) supplemented with starch while highest degradation rate was observed when grown on MSB containing glucose. Meanwhile, peptone was observed to be the optimum nitrogen source for growth and ammonium nitrate for degradation. For physical parameters, it was observed that the fungal consortia had high biomass and degradation rate at pH 5 and agitation speed of 120 rpm. Based on statistical analysis, the biomass of fungal consortia grown on MSB supplemented with different carbon and nitrogen sources, at various pH, and with agitation were not significantly different. On the other hand, ASPE demonstrated the highest biomass and percentage of degradation in all media with various growth parameters.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	668.4 C32 2019 (Browse shelf(Opens below))		Link to resource	Room use only	T-8061		00077314

Thesis (Bachelor of Science in Biology) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS), Department of Biological Sciences

CENIZAL, JAN CLEOMARIE C. "Enhancement of Low Density Polyethylene (LDPE) Plastic Biodegradation through Fungal Consortium". Undergraduate Thesis. Bachelor of Science in Biology, major in Microbiology. Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Indang, Cavite. May 2019. Adviser: Prof. Sherine M. Cruzate.
This study focused on the enhancement of the biodegradation of LDPE plastic using the locally isolated Aspergillus niger, Paecilotnyces spp. and Penicillium spp., as fungal consortium. Compatibility test was done as initial test if these fungi could grow together. Thereafter, they were subjected to in vitro biodegradation assay where the effect of different parameters such as plastic size, carbon sources, nitrogen sources, pH, and agitation on the growth and degradation of fungal consortium were determined after 30 days of incubation. The most promising fungal consortium was the combination of Aspergillus niger and Penicillium spp. (ASPE). In vitro biodegradation assay revealed that this fungal consortium had high biomass when grown on Mineral Salt Broth (MSB) supplemented with starch while highest degradation rate was observed when grown on MSB containing glucose. Meanwhile, peptone was observed to be the optimum nitrogen source for growth and ammonium nitrate for degradation. For physical parameters, it was observed that the fungal consortia had high biomass and degradation rate at pH 5 and agitation speed of 120 rpm.
Based on statistical analysis, the biomass of fungal consortia grown on MSB supplemented with different carbon and nitrogen sources, at various pH, and with agitation were not significantly different. On the other hand, ASPE demonstrated the highest biomass and percentage of degradation in all media with various growth parameters.

Submitted to the University Library July 03, 2019 T-8061