Molecular detection of coxiella burnetii in cattle blood from selected farms in Cavite, Philippines using polymerase cahin reaction / by Joeneil L. Vergaño.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xiii, 51 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 595.4  V58 2019
Online resources: Production credits:
  • College of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health
Abstract: VERGANO, JOENEIL L., Molecular Detection of Coxiella burnetii in Cattle Blood from Selected Farms in Cavite, Philippines Using Polymerase Chain Reaction. Undergraduate Thesis. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. May 2019. Adviser: Emmanuel R. Mago, DVM, MS. In the Philippines, epidemiological studies about the molecular detection of Coxiella burnetii from cattle blood are not yet well-documented and there are only limited research of the pathogen in other animal species. This study was conducted to investigate the presence of Coxiella burnetii on blood samples collected from selected cattle farms within 3 topographical areas in the province of Cavite using Polymerase Chain Reaction. A total of 100 cattle blood samples, 34 from lowland, 43 from central hilly, and 23 from upland were used in this study. Samples were coded according to sex, age, breed, and type of feeding system. Furthermore, type of operation, topography, and municipality were also part of the coding scheme. DNA samples were extracted from blood collected from the lateral ear vein of cattle and were subjected to PCR for the detection of the mammalian Actin gene. All 100 DNA samples that were positive for the mammalian Actin gene (internal control) were subjected to Coxiella burnetii detection using Coxiella-specific primers. However, zero (0) out of 100 DNA samples tested positive for the presence of Coxiella burnetii. The result of this study suggests that the disease is not circulating in the sampled animals during the time of collection. Although past infection is possible, this study was limited in the detection of present bacteremia and future studies will require serological tests to fully determine past infection.
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Thesis (Doctor of Veterinary Medicine) Cavite State University.

Includes bibliographical references.

College of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health

VERGANO, JOENEIL L., Molecular Detection of Coxiella burnetii in Cattle Blood from Selected Farms in Cavite, Philippines Using Polymerase Chain Reaction. Undergraduate Thesis. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. May 2019. Adviser: Emmanuel R. Mago, DVM, MS.
In the Philippines, epidemiological studies about the molecular detection of Coxiella burnetii from cattle blood are not yet well-documented and there are only limited research of the pathogen in other animal species. This study was conducted to investigate the presence of Coxiella burnetii on blood samples collected from selected cattle farms within 3 topographical areas in the province of Cavite using Polymerase Chain Reaction.
A total of 100 cattle blood samples, 34 from lowland, 43 from central hilly, and 23 from upland were used in this study. Samples were coded according to sex, age, breed, and type of feeding system. Furthermore, type of operation, topography, and municipality were also part of the coding scheme. DNA samples were extracted from blood collected from the lateral ear vein of cattle and were subjected to PCR for the detection of the mammalian Actin gene.
All 100 DNA samples that were positive for the mammalian Actin gene (internal control) were subjected to Coxiella burnetii detection using Coxiella-specific primers. However, zero (0) out of 100 DNA samples tested positive for the presence of Coxiella burnetii. The result of this study suggests that the disease is not circulating in the sampled animals during the time of collection. Although past infection is possible, this study was limited in the detection of present bacteremia and future studies will require serological tests to fully determine past infection.

Submitted to the University Library June 24, 2019 T-8114

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