Protein engineering / Kurra Venkata Gopaiah.

Includes bibliographical references and index.

Introduction -- Structure and functions of protein -- Protein design in molecular manufacturing -- Amino acids and proteins structure --
Protein nanotechnology -- Biological functions of proteins -- Protein synthesis -- Enzymatic proteins.

"Protein engineering is the process of developing useful or valuable proteins. It is a young discipline, with much research taking place into the understanding of protein folding and recognition for protein design principles. It is also a product and services market, with an estimated value of $168 billion by 2017. Protein engineering is the conception and production of unnatural polypeptides, often through modification of amino acid sequences that are found in nature. Synthetic protein structures and functions can now be designed entirely on a computer or produced through directed evolution in the laboratory. Protein requiring disulphide bond formation and/or isomerization of proline peptide bonds have to be directed to the periplasm for folding (the environment of the periplasmic space is oxidizing in contrast to the reducing environment of the cytoplasm). There are at least four genes involved in disulphide bond formation and editing in E coli (DsbA, DsbB, DsbC and DsbD) and there is strong evidence that co-expression of the DsbA, (or eukaryotic PDI) gene in the presence of a glutathione redox buffer increases the yield of several disulphide-rick proteins. In this book the subject is presented in a very systematic manner. Simple language is used, diagrams/illustrations are generously used to emphasize reaction. Sites, to indicate reaction pathways. Emphasis is placed on the correlation of the structure of functional group with its properties."--Back cover

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