Antimicrobial, activity and product evaluation of formulated hand sanitizer with propolis from stingless bees Tetragonula biroi fries / by Aera Camille C. Landicho.

By:

Landicho, Aera Camille C [author]

Contributor(s):

Bono, Michele T [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: xiv, 76 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

576 L23 2019

Online resources:

Click here to view abstract and table of contents

Production credits:

College of Arts and Sciences (CAS), Department of Biological Sciences

Abstract: LANDICHO, AERA CAMILLE CABATIAN. Antimicrobial Activity and Product Evaluation of Formulated Hand Sanitizer with Propolis From Tetragonula biroi Friese. Undergraduate Thesis. Bachelor of Science in Biology major in Microbiology, Cavite State University. Indang, Cavite. June 2019. Adviser: Prof. Michele T. Bono. Hand sanitizers are used to reduce viable pathogenic organisms in our hands that could cause and transmit diseases. This study was conducted to formulate hand sanitizer and screen the antimicrobial activity of the formulated hand sanitizer with different concentrations of ethanolic extract of propolis from Tetragonula biroi Friese against selected test organisms (Staphylococcus aureus ATCC 12600, Bacillus subtilis, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Candida albicans 14053). The antimicrobial activity of the ethanolic extract of propolis and formulated hand sanitizer were screened using agar well diffusion assay. The formulated hand sanitizer was compared to commercial hand sanitizer for its antimicrobial activity and acceptability using sensory evaluation. Results showed that different concentrations of ethanolic extract of propolis exhibited inhibitory activity against all bacterial strains used but not on C. albicans 14053, with a minimum inhibitory concentration (MIC) of 40 percent. Similarly, formulated hand sanitizer with 40 percent ethanolic extract of Propolis still exhibited inhibitory activities against all tested bacterial strains. This indicates that propolis extract could be an active component of hand sanitizer. However, the antimicrobial activity of the formulated hand sanitizer decreases after one month of incubation. Despite this, the antimicrobial activity of the formulated hand sanitizer is comparable with the commercially available hand sanitizers in the market. Moreover, the formulated hand sanitizer was highly acceptable than the commercial hand sanitizer, based on the sensory evaluation.

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Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	576 L23 2019 (Browse shelf(Opens below))		Link to resource	Room use only	T-8167		00079682

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Thesis (Bachelor of Science in Biology Major in Microbiology) Cavite State University.

Includes bibliographical references.

College of Arts and Sciences (CAS), Department of Biological Sciences

LANDICHO, AERA CAMILLE CABATIAN. Antimicrobial Activity and Product Evaluation of Formulated Hand Sanitizer with Propolis From Tetragonula biroi Friese. Undergraduate Thesis. Bachelor of Science in Biology major in Microbiology, Cavite State University. Indang, Cavite. June 2019. Adviser: Prof. Michele T. Bono.

Hand sanitizers are used to reduce viable pathogenic organisms in our hands that could cause and transmit diseases. This study was conducted to formulate hand sanitizer and screen the antimicrobial activity of the formulated hand sanitizer with different concentrations of ethanolic extract of propolis from Tetragonula biroi Friese against selected test organisms (Staphylococcus aureus ATCC 12600, Bacillus subtilis, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Candida albicans 14053).

The antimicrobial activity of the ethanolic extract of propolis and formulated hand sanitizer were screened using agar well diffusion assay. The formulated hand sanitizer was compared to commercial hand sanitizer for its antimicrobial activity and
acceptability using sensory evaluation.

Results showed that different concentrations of ethanolic extract of propolis exhibited inhibitory activity against all bacterial strains used but not on C. albicans 14053, with a minimum inhibitory concentration (MIC) of 40 percent. Similarly, formulated hand sanitizer with 40 percent ethanolic extract of Propolis still exhibited inhibitory activities against all tested bacterial strains. This indicates that propolis extract could be an active component of hand sanitizer. However, the antimicrobial activity of the formulated hand sanitizer decreases after one month of incubation. Despite this, the antimicrobial activity of the formulated hand sanitizer is comparable with the commercially available hand sanitizers in the market. Moreover, the formulated hand sanitizer was highly acceptable than the commercial hand sanitizer, based on the sensory evaluation.

Submitted to the University Library 07/04/2019 T-8167

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