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Prevalence, phenotypic characteristics and anti-biograms of the clinical isolates of methicillin resistant (staphylococus aureus (MRSA) among pediatric patients / by Ivy Jane C. Anacan

By:

Anacan, Ivy Jane C [author]

Contributor(s):

Ilagan, Yolanda A [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2008. Cavite State University- Main Campus,Description: xii, 47 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

616.9 An1 2008

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Arts and Science (CAS)

Abstract: ANACAN, IVY JANE CORTEZ. PREVALENCE, PHENOTYPIC CHARACTERISTICS AND ANTIBIOGRAMS OF METHICILLIN RESISTANT Staphylococcus aureus AMONG PEDIATRIC PATIENTS IN GENERAL EMILIO AGUINALDO MEMORIAL HOSPITAL TRECE MARTIRES CITY, CAVITE. Undergraduate Thesis, Bachelor of Science in Biology. Cavite State University, Indang, Cavite. April 2008.Adviser: Dr. Yolanda A. Ilagan A study was conducted to assess the prevalence rate, phenotypic characteristics and antibiograms of Methicillin Resistant Staphylococcus aureus (MRSA) among pediatric patients in General Emilio Aguinaldo Memorial Hospital Trece, Martires City, and Cavite. Nasal swab and questionnaire were provided to each pediatric patient. From 155 children who participated in the study, 88 (57%) were positive for S. aureus while 27 (17%) were positive for MRSA. All isolated S. aureus colonies were yellow, circular and with entire margin. The isolates differed in hemolysis: 17 (63%) were gamma hemolytic while 10 (37%) were beta-hemolytic when grown on Blood Agar Plates. The MRSA isolated from pediatric patient were highly susceptible to kanamycin, tetracycline, gentamicin, norfloxacin, erythromycin, ciprofloxacin, chloramphenicol, vancomycin, lincomycin, clindamycin, rifampicin, and streptomycin .MRSA isolates exhibited low intermediate resistance to B-lactam and non- B-lactam antibiotics. The MRSA isolates resistant in 13-lactam were 15 (56%) while in non- B-lactam,19 (79%) MRSA isolates were resistant to nalidixic acid. Four MRSA isolates exhibited mono-drug resistance, five bi-drug resiatance two each tri- and tetra-drug resistance and one each hepta- and hexa-drug resistance to non-B- lactam antibiotics. The Minimum Inhibitory Concentration (MIC) of 13-lactam and non-13-lactam antibiotics to MRSA isolates ranged from less than 4 1.1g/m1 to more than 328 µg/ml. The demographic characteristics of the pediatric patients such as gender, age, address, residential area and number of siblings were not significantly related with MRSA acquisition among pediatric patients in General Emilio Aguinaldo Memorial Hospital Trece Martires City.

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Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	616.9 An1 2008 (Browse shelf(Opens below))		Link to resource	Room use only	T-3770		00007457

Thesis (BS Biology - - Microbiology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

ANACAN, IVY JANE CORTEZ. PREVALENCE, PHENOTYPIC CHARACTERISTICS AND ANTIBIOGRAMS OF METHICILLIN RESISTANT Staphylococcus aureus AMONG PEDIATRIC PATIENTS IN GENERAL EMILIO AGUINALDO MEMORIAL HOSPITAL TRECE MARTIRES CITY, CAVITE. Undergraduate Thesis, Bachelor of Science in Biology. Cavite State University, Indang, Cavite. April 2008.Adviser: Dr. Yolanda A. Ilagan
A study was conducted to assess the prevalence rate, phenotypic characteristics and antibiograms of Methicillin Resistant Staphylococcus aureus (MRSA) among pediatric patients in General Emilio Aguinaldo Memorial Hospital Trece, Martires City, and Cavite. Nasal swab and questionnaire were provided to each pediatric patient. From 155 children who participated in the study, 88 (57%) were positive for S. aureus while 27 (17%) were positive for MRSA. All isolated S. aureus colonies were yellow, circular and with entire margin. The isolates differed in hemolysis: 17 (63%) were gamma hemolytic while 10 (37%) were beta-hemolytic when grown on Blood Agar Plates. The MRSA isolated from pediatric patient were highly susceptible to kanamycin, tetracycline, gentamicin, norfloxacin, erythromycin, ciprofloxacin, chloramphenicol, vancomycin, lincomycin, clindamycin, rifampicin, and streptomycin .MRSA isolates exhibited low intermediate resistance to B-lactam and non- B-lactam antibiotics. The MRSA isolates resistant in 13-lactam were 15 (56%) while in non- B-lactam,19 (79%) MRSA isolates were resistant to nalidixic acid. Four MRSA isolates exhibited mono-drug resistance, five bi-drug resiatance two each tri- and tetra-drug resistance and one each hepta- and hexa-drug resistance to non-B-
lactam antibiotics. The Minimum Inhibitory Concentration (MIC) of 13-lactam and non-13-lactam antibiotics to MRSA isolates ranged from less than 4 1.1g/m1 to more than 328 µg/ml. The demographic characteristics of the pediatric patients such as gender, age, address, residential area and number of siblings were not significantly related with MRSA acquisition among pediatric patients in General Emilio Aguinaldo Memorial Hospital Trece Martires City.

Submitted to the University Library 04/28/2008 T-3770