Antibiotic sensitivity profile of Salmonella spp. isolates in the liver of chicken (Gallus gallus domesticus, Limm.) from a dressing plant in Trece Martires City / by Chester Joshua V. Saldana

By: Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2008. Cavite State University- Main Campus,Description: xiv, 96 pages : 28 cm. illustrationsContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.513  Sa3 2008
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: SALDANA, CHESTER JOSHUA V. April 2008. Antibiotic Sensitivity Profile of Salmonella spp. Isolates in the Liver of Chicken (Gallus gallus domesticus, Linn.) from a Dressing Plant in Trece Martires City, Cavite. Doctor of Veterinary Medicine, Cavite University, Indang, Cavite. Adviser: Ma. Cynthia N. Rundina- dela Cruz, DVM, The study was conducted to determine the antibiotic sensitivity profile of Salmonella spp. isolates form the liver of 150 bre“ler chickens in a dressing plant in Trece Martires City, Cavite and to determine the prevalence rate of Salmonella spp among the broilers examined. Two hundred-seventy colonies were isolated from the Xylose Lysine Desoxycholate (Difco®). Morphological characterization revealed that 232 of the 270 isolates were gram negative, rod-shaped organisms. The isolates were further characterized biochemically and were found to possess the following Salmonella spp. reactions: Oxidase negative, Alkaline slant/ Acid butt with gas and Hydrogen Sulfide production on Triple Sugar Iron, indole negative with hydrogen sulfide production and motile on Sulfide Indole Motility Medium, Methyl] red positive and Voges-Proskauer test negative, citrate utilization positive, nitrate, v-ease and gelatinase negative. The isolates also fermented glucose, lactose and yield negative result in maltose. The isolates were further characterized serologically using polyvalent O (A-I) and Vi antiserum and result showed that 5 isolates agglutinated antiserum. On the other hand, these 5 isolates did not agglutinate the Vi antiserum. All isolates were found to be susceptible to fosfomycin, gentamicin, trimethoprim-sulfamethoxazole and nitrofurantoin but were resistant to lincomycin, erythromycin, ampicillin and tetracycycline. Intermediate results were obtained with norfloxacin and ciprofloxacin. The prevalence rate of Salmonella spp. in the liver of dressed chickens in a dressing plant in Trece Martires City, Cavite was found to be 3.3%. It is therefore recommended that proper handling practices be emphasized repeatedly from the farm to the household by application of Hazard Analysis Critical Control Point (HACCP) to prevent the organism from entering the human food chain and that indiscriminate use of antibiotics be avoided in food animals.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.513 Sa3 2008 (Browse shelf(Opens below)) Link to resource Room use only T-3734 00007504

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

SALDANA, CHESTER JOSHUA V. April 2008. Antibiotic Sensitivity Profile of Salmonella spp. Isolates in the Liver of Chicken (Gallus gallus domesticus, Linn.) from a Dressing Plant in Trece Martires City, Cavite. Doctor of Veterinary Medicine, Cavite University, Indang, Cavite. Adviser: Ma. Cynthia N. Rundina- dela Cruz, DVM,
The study was conducted to determine the antibiotic sensitivity profile of Salmonella spp. isolates form the liver of 150 bre“ler chickens in a dressing plant in Trece Martires City, Cavite and to determine the prevalence rate of Salmonella spp among the broilers examined. Two hundred-seventy colonies were isolated from the Xylose Lysine Desoxycholate (Difco®). Morphological characterization revealed that 232 of the 270 isolates were gram negative, rod-shaped organisms. The isolates were further characterized biochemically and were found to possess the following Salmonella spp. reactions: Oxidase negative, Alkaline slant/ Acid butt with gas and Hydrogen Sulfide production on Triple Sugar Iron, indole negative with hydrogen sulfide production and motile on Sulfide Indole Motility Medium, Methyl] red positive and Voges-Proskauer test negative, citrate utilization positive, nitrate, v-ease and gelatinase negative. The isolates also fermented glucose, lactose and yield negative result in maltose. The isolates were further characterized serologically using polyvalent O (A-I) and Vi antiserum and result showed that 5 isolates agglutinated antiserum. On the other hand, these 5 isolates did not agglutinate the Vi antiserum.
All isolates were found to be susceptible to fosfomycin, gentamicin, trimethoprim-sulfamethoxazole and nitrofurantoin but were resistant to lincomycin, erythromycin, ampicillin and tetracycycline. Intermediate results were obtained with norfloxacin and ciprofloxacin.

The prevalence rate of Salmonella spp. in the liver of dressed chickens in a dressing plant in Trece Martires City, Cavite was found to be 3.3%. It is therefore recommended that proper handling practices be emphasized repeatedly from the farm to the household by application of Hazard Analysis Critical Control Point (HACCP) to prevent the organism from entering the human food chain and that indiscriminate use of antibiotics be avoided in food animals.

Submitted to the University Library 04-03-2008 T-3734

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