Antimicrobial activity of wood vinegar substrates against selected gram-negative and gram-positive bacteria / Ma. Eliza Romana C. Asuncion, Roxanne D. Rodriguez and Rona Arianne B. Romanes.
Material type:
TextLanguage: English Publication details: Indang, Cavite, Cavite State University - Main Campus 2013.Description: xvii, 65 pages : illustrations : 28 cmContent type: - text
- unmediated
- volume
- 579.3 As9 2013
- College of Nursinbg (CON)
| Item type | Current library | Collection | Call number | Materials specified | Copy number | Status | Notes | Date due | Barcode |
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Theses / Manuscripts
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 579.3 As9 2013 (Browse shelf(Opens below)) | Room use only | T-4891 | 00008212 | |||
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Theses / Manuscripts
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 579.3 As9 2013 (Browse shelf(Opens below)) | c2 | Room use only | T-5158 | 00074577 |
Thesis ( BS Medical Technology ) Cavite State University.
Includes bibliographical references.
College of Nursinbg (CON)
ASUNCION, MA. ELIZA ROMANA C., ROXANNE D. RODRIGUEZ, RONA ARIANNE B. ROMANES. Antimicrobial Activity Of Wood Vinegar Substrates Against Selected Gram- Negative And Gram-Positive Bacteria. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, August 2012. Adviser: Dr. Adelaida Sangalang
This study was conducted from April 2012 to August 2012 at the Microbiology Laboratory of the Medical Technology Department of the College of Nursing at Cavite State University in Indang Cavite. The study aimed to determine the antimicrobial activity of wood vinegar substrates against selected gram-negative and gram-positive bacteria. Adequate amount of wood vinegar substrates from fresh coconut husks, bamboo stems, and kakawate leaves were collected and processed at the College of Agriculture, Forestry, Environment and Natural R esources (CAFENR). Each wood vinegar was diluted with distilled water to obtain concentrations of 20, 40, 60, and 80 percent v/v. Clindamycin, gentamicin and distilled water served as controls. The antimicrobial activities of the three wood vinegars obtained from the different substrates were determined using agar-well diffusion method. The diameter of the zone of inhibition was measured after 24 hours of incubation using vernier caliper. Zones of inhibition were categorized as resistant, intermediate, and susceptible based on the Zone of Diameter Interpretative Standard (ZDIS). Results showed that the bamboo wood vinegar substrate, bamboo of different concentrations contained bactericidal properties that could inhibit the growth of gram-positive and gram-negative bacteria. The phytochemical constituents of bamboo wood vinegar that contributed to its bactericidal activity were triterpenes, saponins, tannins, and glycosides. On the other hand, kakawate wood vinegar was only susceptible to Salmonella typhi. Kakawate wood vinegar consisted of phytochemical components such as triterpenes, glycosides and tannins. Coconut wood vinegar consisting of triterpenes and tannins did not exhibit susceptibility to the four tested bacteria. The 80 percent concentration of bamboo substrate exhibited the best concentration to all bacteria tested. This result was similar to the kakawate wood vinegar, wherein its 80 percent concentration showed the highest bactericidal activity Salmonella typhi. Bamboo wood vinegar tested to Escherichia coli was comparable to the standard antibiotics. However, the bactericidal activities of coconut and kakawate wood vinegar tested to the other bacteria were not comparable to the bactericidal activities of clindamycin and gentamicin.
Submitted to the University Library 05/06/2013 T-4891
Submitted to the University Library 05/06/2013 T-5158 copy 2