Introgression of phosphorus uptake (Pup1) and submergence tolerance (Sub1) genes in IR64 rice variety (Oryza sativa L.) / by Deolito M. Bicua Jr.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2015.Description: xii, 76 pages : 28 cm. illustrationsContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 576.5  B47 2014
Online resources: Production credits:
  • College of Arts and Science (CAS)
Abstract: BICUA, DEOLITO JR. M. April 2014. "INTROGRESSION OF PHOSPHORUS UPTAKE (Pupl) AND SUBMERGENCE TOLERANCE (Subl) GENES IN 1R64 RICE VARIETY (Oryza saliva L.)". Undergraduate Thesis, Bachelor of Science in Biology Major in General Biology, College of Arts and Sciences, Cavite State University, Indang, Cavite. Adviser: Dr. Miriam D. Baltazar; Co-adviser: Dr. Joong Hyoun Chin Phosphorus deficiency and submergence are two of the major abiotic constraints in rice production particularly in Asia. Pup! and Subl the quantitative trait loci (QTL) known to confer tolerance to P-deficiency and submergence respectively were aimed to introgress in one genetic background of the elite variety IR.64. Ninety four randomly selected F2 lines derived from the cross of 1R64-Pupl and 1R64-Subl were used for genotyping using Pupl molecular markers, K46-1 and K29-1 and Sub] molecular marker, SC3. Results showed that the 2 markers followed the expected Mendelian segregation (IA: 2H: 1B). Three Pupl-Subl lines, 2 Pupl lines and 3 Sub/ lines were developed from the initial. F2 selections through confirmatory genotyping for their F3 progenies. Phenotyping under irrigated low P conditions revealed that Pup1 -Sub lines developed longer root length and larger root surface area comparable with Kasalath, the tolerant check and 1R64-Pupl, the P-deficiency tolerant parent but developed lower shoot biomass comparable to IR64-Subl and the susceptible check, IR64. Phenotyping for submergence showed that much stress to the test lines which is accounted for only FR13A had 60% survival rate and none of the Pupl-Subl lines survived the stress. Minimal growth of Subl lines 78 and 51 in the experiment were observed and only few plants survived after prolonged period of observations. The study was able to develop IR64-Pup 1-Sub 1 lines at molecular level through marker-assisted selection however, under irrigated low P conditions, Pupl-Subl lines were able to develop larger root system but developed lower shoot biomass. These lines are not able to manifest Subl phenotype under submergence stress in the given set-up for this study.
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Thesis ( BS Biology ) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

BICUA, DEOLITO JR. M. April 2014. "INTROGRESSION OF PHOSPHORUS UPTAKE (Pupl) AND SUBMERGENCE TOLERANCE (Subl) GENES IN 1R64 RICE VARIETY (Oryza saliva L.)". Undergraduate Thesis, Bachelor of Science in Biology Major in General Biology, College of Arts and Sciences, Cavite State University, Indang, Cavite. Adviser: Dr. Miriam D. Baltazar; Co-adviser: Dr. Joong Hyoun Chin
Phosphorus deficiency and submergence are two of the major abiotic constraints in rice production particularly in Asia. Pup! and Subl the quantitative trait loci (QTL) known to confer tolerance to P-deficiency and submergence respectively were aimed to introgress in one genetic background of the elite variety IR.64. Ninety four randomly selected F2 lines derived from the cross of 1R64-Pupl and 1R64-Subl were used for genotyping using Pupl molecular markers, K46-1 and K29-1 and Sub] molecular marker, SC3. Results showed that the 2 markers followed the expected Mendelian segregation (IA: 2H: 1B). Three Pupl-Subl lines, 2 Pupl lines and 3 Sub/ lines were developed from the initial. F2 selections through confirmatory genotyping for their F3 progenies. Phenotyping under irrigated low P conditions revealed that Pup1 -Sub lines developed longer root length and larger root surface area comparable with Kasalath, the tolerant check and 1R64-Pupl, the P-deficiency tolerant parent but developed lower shoot biomass comparable to IR64-Subl and the susceptible check, IR64. Phenotyping for submergence showed that much stress to the test lines which is accounted for only FR13A had 60% survival rate and none of the Pupl-Subl lines survived the stress. Minimal growth of Subl lines 78 and 51 in the experiment were observed and only few plants survived after prolonged period of observations.
The study was able to develop IR64-Pup 1-Sub 1 lines at molecular level through marker-assisted selection however, under irrigated low P conditions, Pupl-Subl lines were able to develop larger root system but developed lower shoot biomass. These lines are not able to manifest Subl phenotype under submergence stress in the given set-up for this study.

Submitted copy to the University Library. 04/20/2015 T-5659

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