Cytotoxic activities of the extracts and the fractions from pertroleum nut (Pittosporum resiniferum) / by Alexandria Louisse V. Gaddi, Jacob M. Gomez and Lenar Rae J. Guiao.
Material type:
TextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xvi, 64 pages : illustrations ; 28 cmContent type: - text
- unmediated
- volume
- 581.4 G11 2015
- Science High School, College of Education (CED)
| Item type | Current library | Collection | Call number | Materials specified | URL | Status | Notes | Date due | Barcode |
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Theses / Manuscripts
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 581.4 G11 2015 (Browse shelf(Opens below)) | Link to resource | Room use only | RS-733 | 00009325 |
Research Study (Science High School) Cavite State University
Includes bibliographical references.
Science High School, College of Education (CED)
GADDIL ALEXANDRIA LOUISSE V., GOMEZ, JACOB M., and GUIAO, LENAR RAE J. Cytotoxic Activities of the Extracts and the Fractions from Petroleum Nut (Pittosporum resiniferum). Research Study (General Science Curriculum) Science High School, College of Education, Cavite State University, Indang, Cavite. Adviser: Dr. Hosea dL. Matel.
The study was conducted from July 2014 to March 2015 at the Research Center Laboratory, Cavite State University, Indang, Cavite to determine the cytotoxic activities of the extracts and the fractions from petroleum nut. Specifically, it aimed to: determine the best extraction method for petroleum nut flesh; determine the best extracting solvent from the best extraction method; and determine the percentage lethalities of the brine shrimps to the extracts and the fractions from petroleum nut flesh. There were two methods used to extract the components from petroleum nut flesh, namely soaking method and Soxhlet extraction. Thin Layer Chromatography (TLC) was used to determine the best extraction method and best extracting solvent.
The number of components before and after charring the TLC profiles of the extracts in Soxhlet extraction was higher than the number of compounds in the extracts using the soaking method. The extracting solvent with the most number of compounds before and after charring in the Soxhlet extraction was the DCM extract, thus making it the best extracting solvent. The MeOH and hexane extracts from Soxhlet method were the second and third best extracting solvents that were also considered. The DCM extract underwent column chromatography thrice and collected 36 fractions from it. The fractions with the same number of compounds and their corresponding Rf values were grouped together. The best fractions according to their color were Fractions 3, 4, 16, and 18.
Brine Shrimp Lethality Assay (BSLA) was used to determine the cytotoxic activity of the extracts and fractions. The control groups used were MeOH for negative control and 0.02 g/mL and 2 pg/mL of brewed coffee dissolved in distilled water were used as positive controls. The cytotoxic activities of the extracts were all the same having an average percentage lethality of 30. Among the fractions, Fraction 18 yielded the highest average percentage lethality which was 0 percent compared to other fractions with -3.33 percent average lethality. On the other hand, there was a significant difference between the cytotoxic activities of the extracts and fractions. The cytotoxic activity of the extracts was higher compared to that of the fractions.
Submitted copy to the University Library. 08/18/2015 RS-733