In vitro evaluation of antibacterial property of sampa-sampalukan (Phyllanthus niruri L.) crude leaf extract against tracheal isolates broilers / by Aina May V. Madalansacay, Mhar Florence S. Poniente and Marnelle D. Tablatin.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xv, 42 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.5  M26 2015
Online resources: Production credits:
  • College of Agriculture, Food, Environment and Natural Resources (CAFENR)
Abstract: MADLANSACAY, AINA MAY V., PONIENTE, MIIAR FLO 1"‘ENCE S., TABLATIN, MARNELLE P. In Vitro Evaluation of Antibacterial Property of Sampa-sampalukan (Phyllanthus niruri L.) Crude Leaf Extract Against Tracheal Isolates of Broilers. Undergraduate Thesis. Bachelor of Science in Agriculture Major in Animal Science. Cavite State University, Indang, Cavite. April 2015. Adviser: Prof. Teresita M. Labrador. The study was conducted from November 2014 to January 2015 at the College of Veterinary Medicine and Biomedical Sciences to evaluate the antibacterial property of P. niruri_ crude leaf extract against the tracheal isolates of broilers. Specifically it aimed to: 1. screen the inhibitory effect of P. niruri crude leaf extract against the tracheal isolates of broilers; 2. determine the best concentration of P. niruri crude leaf extract against tracheal isolates of broilers; and 3. determine which bacteria will resist to the P. niruri crude leaf extract. Collection of isolates from the trachea of broilers, pouring of the pure culture on the plates and embedding of Phylianthus niruri and the antibiotic disc and reading of the results after 24 and 48 hours were done to gather pertinent data. Five heads of 24 days old broilers were used. The bacteria identified were; Bacillus cereus, Staphylococcus aureus, Streptococcus pyogenes, and Klehs.lella pneumoniae. The study used 87.5 grams of fresh P. niruri and extracted with 600 ml of 70% ethanol using soxhlet apparatus. There were four treatments used in the study namely: TO- Lincomycin, T1- 50% P. niruri crude leaf extract, T2- 75% P. niruri crude leaf extract, and T3- 100% P. niruri crude leaf extract. Based on the data as well as the results of statistical analysis it could be inferred that the antibacterial properties of P. niruri crude leaf extract has an antibacterial effect. The antibacterial effect of P. niruri crude leaf extract tends to give a clue to the drug resistant strains of microorganisms causing respiratory disease and thus provides the possibility of drug development for such poultry diseases. The P. nirzeri crude leaf extract showed an intermediate effect on the bacteria which were isolated from the trachea of the broilers and therefore said to have an antibacterial effect. It has an antibacterial property that inhibits the growth of bacteria of broilers. One hundred percent (100%) concentration can be used for preventive medication in neutralizing the level of bacteria on broilers is recommended.
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Thesis (BS Agriculture--Animal Science) Cavite State University

Includes bibliographical references.

College of Agriculture, Food, Environment and Natural Resources (CAFENR)

MADLANSACAY, AINA MAY V., PONIENTE, MIIAR FLO 1"‘ENCE S., TABLATIN, MARNELLE P. In Vitro Evaluation of Antibacterial Property of Sampa-sampalukan (Phyllanthus niruri L.) Crude Leaf Extract Against Tracheal Isolates of Broilers. Undergraduate Thesis. Bachelor of Science in Agriculture Major in Animal Science. Cavite State University, Indang, Cavite. April 2015. Adviser: Prof. Teresita M. Labrador.

The study was conducted from November 2014 to January 2015 at the College of Veterinary Medicine and Biomedical Sciences to evaluate the antibacterial property of P. niruri_ crude leaf extract against the tracheal isolates of broilers. Specifically it aimed to: 1. screen the inhibitory effect of P. niruri crude leaf extract against the tracheal isolates of broilers; 2. determine the best concentration of P. niruri crude leaf extract against tracheal isolates of broilers; and 3. determine which bacteria will resist to the P. niruri crude leaf extract. Collection of isolates from the trachea of broilers, pouring of the pure culture on the plates and embedding of Phylianthus niruri and the antibiotic disc and reading of the results after 24 and 48 hours were done to gather pertinent data. Five heads of 24 days old broilers were used. The bacteria identified were; Bacillus cereus, Staphylococcus aureus, Streptococcus pyogenes, and Klehs.lella pneumoniae.

The study used 87.5 grams of fresh P. niruri and extracted with 600 ml of 70% ethanol using soxhlet apparatus. There were four treatments used in the study namely: TO- Lincomycin, T1- 50% P. niruri crude leaf extract, T2- 75% P. niruri crude leaf extract, and T3- 100% P. niruri crude leaf extract. Based on the data as well as the results of statistical analysis it could be inferred that the antibacterial properties of P. niruri crude leaf extract has an antibacterial effect. The antibacterial effect of P. niruri crude leaf extract tends to give a clue to the drug resistant strains of microorganisms causing respiratory disease and thus provides the possibility of drug development for such poultry diseases.
The P. nirzeri crude leaf extract showed an intermediate effect on the bacteria which were isolated from the trachea of the broilers and therefore said to have an antibacterial effect. It has an antibacterial property that inhibits the growth of bacteria of broilers. One hundred percent (100%) concentration can be used for preventive medication in neutralizing the level of bacteria on broilers is recommended.

Submitted copy to the University Library. 11/18/2015 T-5764

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