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Isolation and antibiotic sensitivity testing of uropathogenic EScherichia coli (UPEC) in the urine of clinically sick and apparently healthy dogs / by Cleo Angelica B. Mojares.

By:

Mojares, Cleo Angelica B [author]

Contributor(s):

Dela Cruz, Ma. Cynthia R [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2015.Description: xiv, 93 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

636.7 M72 2015

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biomedical Sciences (CVMBS)

Abstract: MOJARES, CLEO ANGELICA B. Isolation and Antibiotic Sensitivity Testing of Uropathogenic Escerichia coil (UPEC) in the Urine of Clinically Sick and Apparently Healthy Dogs (Canis lupus familiaris) from a Veterinary Hospital in Makati City, Philippines. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2015. Adviser: Ma. Cynthia dela Cruz, DVM. This study was done to isolate uropathogenic Escherichia coil (UPEC) in the urine samples of clinically sick and apparently healthy dogs and determine its antibacterial susceptibility profile. A total of 90 urine samples obtained by catheterization from clinically sick and apparently healthy dogs from a veterinary hospital in Makati City were examined bacteriologically. Total E. coil and coliform counts from the urine samples were determined using 3MTM PetrifilmTM E. colil coliform count plate. Twenty one samples showed growth on 3MTm PetrifilmTM E. colil coliform count plate. Morphological, biochemical, hemolysis detection as well as antibiotic sensitivity testing were done to identify and determine the antibiotic sensitivity profile of E. coil. The organism showed sensitivity to the following antibiotics: amikacin, cefoxitin, and chloramphenicol. On the other hand, E. coil isolates were completely resistant to clindamycin and erythromycin. Out of 90 animals sampled, 15 dogs were found positive for UPEC, having a prevalence of 16.7 %. Results on antibiotic susceptibility test across total E. coil and coliform counts revealed significant association for susceptibility to cefoxitin. Also, the associations between the total E. coli and coliform count revealed highly significant results with the prevalence of E. coil. This study showed that multidrug resistant UPEC isolates were found in domestic dogs and can be a source of infection to other animals and humans.

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Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	636.7 M72 2015 (Browse shelf(Opens below))		Link to resource	Room use only	T-5866		00009579

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Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (CVMBS)

MOJARES, CLEO ANGELICA B. Isolation and Antibiotic Sensitivity Testing of Uropathogenic Escerichia coil (UPEC) in the Urine of Clinically Sick and Apparently Healthy Dogs (Canis lupus familiaris) from a Veterinary Hospital in Makati City, Philippines. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2015. Adviser: Ma. Cynthia dela Cruz, DVM.

This study was done to isolate uropathogenic Escherichia coil (UPEC) in the urine samples of clinically sick and apparently healthy dogs and determine its antibacterial susceptibility profile. A total of 90 urine samples obtained by catheterization from clinically sick and apparently healthy dogs from a veterinary hospital in Makati City were examined bacteriologically. Total E. coil and coliform counts from the urine samples were determined using 3MTM PetrifilmTM E. colil coliform count plate. Twenty one samples showed growth on 3MTm PetrifilmTM E. colil coliform count plate. Morphological, biochemical, hemolysis detection as well as antibiotic sensitivity testing were done to identify and determine the antibiotic sensitivity profile of E. coil. The organism showed sensitivity to the following antibiotics: amikacin, cefoxitin, and chloramphenicol. On the other hand, E. coil isolates were completely resistant to clindamycin and erythromycin. Out of 90 animals sampled, 15 dogs were found positive for UPEC, having a prevalence of 16.7 %.

Results on antibiotic susceptibility test across total E. coil and coliform counts revealed significant association for susceptibility to cefoxitin. Also, the associations between the total E. coli and coliform count revealed highly significant results with the prevalence of E. coil. This study showed that multidrug resistant UPEC isolates were found in domestic dogs and can be a source of infection to other animals and humans.

Submitted copy for the University Library T-5866