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Phytochemical analysis and antimicrobial properties of petroleum nut (pittosporum resiniferum) bark extracts / by Tricia Corinne L. Anuat, Arlene V. Constantino and Kyla O. Marasigan.

By:

Anuat, Tricia Corinne L [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xiv, 61 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

581.4 An8 2016

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

Science High School, College of Education (CED)

Abstract: ANUAT, TRICIA CORINNE L., CONSTANTINO, ARLENE V. and MARASIGAN, SOFIA KYLA O. Phytochemical Analysis and Antimicrobial Properties of Petroleum Nut (Pittosporum resiniferum) Bark Extracts. Research Study. Science High School (General Science Curriculum), College of Education, Cavite State University, Indang, Cavite. April 2016. Adviser: Prof. Agnes B. Alimboyoguen. The study entitled “Phytochemical Analysis and Antimicrobial Properties of Petroleum Nut (Pittosporum resiniferum) Bark Extracts” was conducted to characterize and evaluate the bark extract of petroleum nut. Specifically, it aimed to: determine which solvent can dissolve the dichloromethane fractions of the bark of petroleum nut; identify the phytochemical constituents present from the bark of petroleum nut; and test the antimicrobial properties of dichloromethane (DCM) extract from the bark of petroleum nut. The barks of petroleum nut were gathered from La Trinidad, Benguet. Tools and equipment, along with the materials necessary in conducting the study were obtained from the Research Center Laboratory, College of Veterinary Medicine and Biomedical Sciences Laboratory, and Department of Biological Sciences Supply Room, Cavite State University. The isolates were obtained using column chromatography. Phytochemical analysis was also determined by identifying the presence of flavonoids, glycosides, saponins and terpenoids. Furthermore, the antimicrobial activity was tested using disk diffusion method. Results showed that all the ten fractions from dichloromethane extracts were soluble in both dichloromethane (DCM) and petroleum ether. Further, results revealed that only terpenoid were present in all the isolates. Statistical test also revealed that among the ten dichloromethane fractions of dichloromethane extract from the bark of petroleum nut, 70% Acetone in DCM showed the best antimicrobial activity against Escherichia coli while 90% Acetone in DCM showed the best antimicrobial activity against Pseudomonas aeruginosa.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	581.4 An8 2016 (Browse shelf(Opens below))		Link to resource	Room use only	RS-764		00009813

Research Study (Science High School) Cavite State University

Includes bibliographical references.

Science High School, College of Education (CED)

ANUAT, TRICIA CORINNE L., CONSTANTINO, ARLENE V. and MARASIGAN, SOFIA KYLA O. Phytochemical Analysis and Antimicrobial Properties of Petroleum Nut (Pittosporum resiniferum) Bark Extracts. Research Study. Science High School (General Science Curriculum), College of Education, Cavite State University, Indang, Cavite. April 2016. Adviser: Prof. Agnes B. Alimboyoguen.

The study entitled “Phytochemical Analysis and Antimicrobial Properties of Petroleum Nut (Pittosporum resiniferum) Bark Extracts” was conducted to characterize and evaluate the bark extract of petroleum nut. Specifically, it aimed to: determine which solvent can dissolve the dichloromethane fractions of the bark of petroleum nut; identify the phytochemical constituents present from the bark of petroleum nut; and test the antimicrobial properties of dichloromethane (DCM) extract from the bark of petroleum nut. The barks of petroleum nut were gathered from La Trinidad, Benguet. Tools and equipment, along with the materials necessary in conducting the study were obtained from the Research Center Laboratory, College of Veterinary Medicine and Biomedical Sciences Laboratory, and Department of Biological Sciences Supply Room, Cavite State University.

The isolates were obtained using column chromatography. Phytochemical analysis was also determined by identifying the presence of flavonoids, glycosides, saponins and terpenoids. Furthermore, the antimicrobial activity was tested using disk
diffusion method.

Results showed that all the ten fractions from dichloromethane extracts were soluble in both dichloromethane (DCM) and petroleum ether. Further, results revealed that only terpenoid were present in all the isolates.

Statistical test also revealed that among the ten dichloromethane fractions of dichloromethane extract from the bark of petroleum nut, 70% Acetone in DCM showed the best antimicrobial activity against Escherichia coli while 90% Acetone in DCM showed the best antimicrobial activity against Pseudomonas aeruginosa.

Submitted copy to the University Library. 10/11/2016 RS-764