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Screening for the antimicrobial activity of oregano (Origanum vulgare) plant extract against Escherichia coli isolates from piglets / by Kayla Mellen M. Satero.

By:

Santero, Kayla Mellen M [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xii, 51 pages : 28 cm. illustrationsContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

581.6 Sa5 2016

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

Science High School, College of Education (CED)

Abstract: SANTERO, KAYLA MELLEN M. AND SILAN, NAOMI ELY'S C. Screening for the Antimicrobial Activity Of Oregano (Origanum Vulgare) Plant Extract Against Escherichia Coli Isolates from Piglets. Research study (General Science Curriculum). Science High School, College of Education, Cavite State University. Indang, Cavite. April 2016. Adviser: Dr. Chester Joshua V. Saldana. The study was conducted to screen the antimicrobial activity of oregano (Origanum vulgare) plant extract against Escherichia coliform piglets. Specifically, it aimed to: (1) identify the morphological and physiological characteristics of E. coli from fecal samples of piglets, (2) determine the physical properties of the aqueous and dichloromethane oregano extract in terms of odor, color and form; (3) determine the antimicrobial activities of the aqueous and dichloromethane oregano extract by agar well diffusion method against F.coli isolates; (4) determine which among the parts of the oregano plant best inhibit the growth of the isolated £. coli; (5) compare the effect of dichloromethane and aqueous oregano extract against E. coli isolates. Seven fecal samples from 3-day old piglets exhibiting diarrhea was sampled. Bacterial isolates from fecal samples of piglets were subjected to morphological and physiological characterization tests to identify the organism. The Jeaf and stem of oregano plant was collected, dried and processed. The extracts were prepared in two different processes: extraction using dichloromethane (DCM) and by infusion. Results revealed that the bacterial isolates are gram negative, rod shaped organisms that appear singly upon microscopic examination. All physiological tests show positive results typically of Escherichia coli. Agar well diffusion test was performed in all isolates for antimicrobial activity testing and zone of inhibition produced after incubating were recorded. Results also revealed that the dichloromethane (DCM) oregano extracts produced the highest zone inhibition both for the leaves and stem extracts and are statistically higher when compared with extracts derived from infusion. Moreover, the oregano extracts from DCM produced significantly higher inhibition compared to control (tetracycline). With regards to parts of the oregano plant, the zone of inhibition of stem fraction from DCM produced significantly higher values than the zone of inhibition by infusion for both stem and leaves. This study revealed that the extracts from leaves and stem of oregano exhibit antimicrobial activity.

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Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	581.6 Sa5 2016 (Browse shelf(Opens below))		Link to resource	Room use only	RS-773		00009821

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Research Study (Science High School) Cavite State University

Includes bibliographical references.

Science High School, College of Education (CED)

SANTERO, KAYLA MELLEN M. AND SILAN, NAOMI ELY'S C. Screening for the Antimicrobial Activity Of Oregano (Origanum Vulgare) Plant Extract Against Escherichia Coli Isolates from Piglets. Research study (General Science Curriculum). Science High School, College of Education, Cavite State University. Indang, Cavite. April 2016. Adviser: Dr. Chester Joshua V. Saldana.

The study was conducted to screen the antimicrobial activity of oregano (Origanum vulgare) plant extract against Escherichia coliform piglets. Specifically, it aimed to: (1) identify the morphological and physiological characteristics of E. coli from fecal samples of piglets, (2) determine the physical properties of the aqueous and dichloromethane oregano extract in terms of odor, color and form; (3) determine the antimicrobial activities of the aqueous and dichloromethane oregano extract by agar well diffusion method against F.coli isolates; (4) determine which among the parts of the oregano plant best inhibit the growth of the isolated £. coli; (5) compare the effect of dichloromethane and aqueous oregano extract against E. coli isolates.

Seven fecal samples from 3-day old piglets exhibiting diarrhea was sampled. Bacterial isolates from fecal samples of piglets were subjected to morphological and physiological characterization tests to identify the organism. The Jeaf and stem of oregano plant was collected, dried and processed. The extracts were prepared in two different processes: extraction using dichloromethane (DCM) and by infusion.

Results revealed that the bacterial isolates are gram negative, rod shaped organisms
that appear singly upon microscopic examination. All physiological tests show positive results typically of Escherichia coli. Agar well diffusion test was performed in all isolates for antimicrobial activity testing and zone of inhibition produced after incubating were recorded.

Results also revealed that the dichloromethane (DCM) oregano extracts produced the highest zone inhibition both for the leaves and stem extracts and are statistically higher when compared with extracts derived from infusion. Moreover, the oregano extracts from DCM produced significantly higher inhibition compared to control (tetracycline). With regards to parts of the oregano plant, the zone of inhibition of stem fraction from DCM produced significantly higher values than the zone of inhibition by infusion for both stem and leaves. This study revealed that the extracts from leaves and stem of oregano exhibit antimicrobial activity.

Submitted copy to the University Library. 10/24/2016 RS-773