Immune response of Nile tilapia on Aeromonas hydrophila isolated from Taal lake / by Jenielyn V. Poniente.
Material type: TextLanguage: English Publication details: Indang, Cavite, 2002. Cavite State University- Main Campus,Description: xii, 53 pages : illustrations ; 28 cmContent type:- text
- unmediated
- volume
- 639.31 P77 2002
- College of Arts and Science (CAS)
Item type | Current library | Collection | Call number | Materials specified | Status | Notes | Date due | Barcode |
---|---|---|---|---|---|---|---|---|
Theses / Manuscripts | Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 639.31 P77 2002 (Browse shelf(Opens below)) | Room use only | T-2668 | 00006499 |
Thesis (B.S. Biology--Microbiology) Cavite State University
Includes bibliographical references.
College of Arts and Science (CAS)
PONIENTE, JENIELYN VELASQUEZ, April, 2002, " Immune Response of IMapia (Oreoehromis niloticus) on Aeromonas hydrophila Isolated from Taal Lake", An Undergraduate Thesis, Bachelor of Science in Biology, major in Microbiology, Cavite State University, Indang, Cavite. Advisers: Mr. Francisco M. Heralde III and Dr. Yolanda A. Ilagan
Immune response of Nile tilapia (Oreochromis niloticus) on Aeromonas hydrohila isolated from Taal Lake was conducted. This study aimed to: optimize the conditions for tilapia blood sample extraction and processing, antibody quantification and white and red blood cells detection and quantification and to determine the effect of Aeromonas hydrophila infection on the tilapia antibody titers and levels of white and red blood cells, physical appearance and mortality rate. Four treatments were employed: sterile distilled water as control, heat-killed bacteria, formalin- killed bacteria and bacteria suspended in saline solution at a rate of 2.15 x 105 cells per ml. It was found out that lOul of inoculum per gram of fish can be used as a standard concentration of antigen injected to fish considering their body weight. Positive agglutination was observed in Nile tilapia after injection of Aeromonas hydrophila. Increase in antibody titer of fish at all treatments was detected on the third day of post- inoculation up to ten days. Rapid decline of antibody production was observed on the tenth day of post- inoculation and onwards. Red blood cell (RBC) of Nile tilapia at different treatments except for the control declines after six days post- inoculation Decrease in leukocyte count of fish given heat-killed, formalin-killed and live bacteria suspended in 0.85% saline solution was observed after stimulation of antigen. During the conduct of the study, all fish samples exhibited symptoms of disease caused by Aeromona hydrophila such as exophthalmia, fin rot and loss of scales.
Submitted to the University Library 05/10/2007 T-2668