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Isolation and antidiabetic activity of the chemical components from bignail leaves (Antidesma bunius) / by Ronald Mari Jerald R. Caspillan.

By:

Caspilan, Ronald Mari Jerald R [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2014. Cavite State University- Main Campus,Description: x, 53 pages : 28 cm. illustrations ; Content type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

616.462 C27 2014

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

Science High School, College of Education (CED)

Abstract: CASPILLAN, RONALD MARI JERARD R., ERANA, CHRISTIAN EARLP., REYES, GIERONE LIANNI A. Isolation and Antidiabetic Activity of the chemical components from Bignai Leaves (Antidesma bunius). Research Study (General Science Curriculum), Science High School, College of Education, Cavite State University, Indang, Cavite, April 2014. Adviser: Mrs. Agnes Alimboyoguen This study entitled “Isolation and Antidiabetic activity of the chemical components from Bignai Leaves (Anfidesma bunius)” was conducted to: determine the physical properties of isolates from bignai in terms of color, odor, texture, and solubility; determine the phytochemical components from bignai and; test the antidiabetic activities of each component present in bignai. The isolates were obtained using column chromatography and their physical properties were evaluated in terms of odor, color, texture and solubility. Phytochemical analysis was also determined by identifying the presence of glycosides, saponins, flavonoids and terpenoids from the bignai isolates. Furthermore, the antidiabetic activity was tested using the oral glucose tolerance test on the laboratory rats. The findings revealed brown color from the 30%, 60%, 90% and 100% acetone. The results also revealed that all isolates have a pungent odor and greasy texture. The results showed that the isolate from 100% DCM was found insoluble in both distilled water and petroleum ether but soluble in both methanol and ethanol. Meanwhile, the 30%, 60%, 90% and 100% acetone were found insoluble in distilled water but soluble in petroleum ether, methanol, and ethanol. The results revealed that the isolates from DCM, 30%, 60% and 100% acetone have glycosides. On the other hand, the presence of saponins was found only from 60%, 90% and 100% acetone. The presence of flavonoid was found in 30%, 60% and 100% acetone. In addition, terpenoids was identified in DCM, 30%, 60% and 100% acetone. The mean zone of glucose reduction of the glucose reduction of the DCM was 22.60, the 30% acetone was 20.40. the 60% acetone was 17.40, the 90% acetone was 8.80, and the 100% acetone was 10.60. Based on the results, the isolates showed glucose reduction but exhibited low antidiabetic activity.

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Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	616.462 C27 2014 (Browse shelf(Opens below))		Link to resource	Room use only	RS-701		00008983

Research Study (Science High School) Cavite State University

Includes bibliographical references.

Science High School, College of Education (CED)

CASPILLAN, RONALD MARI JERARD R., ERANA, CHRISTIAN EARLP., REYES, GIERONE LIANNI A. Isolation and Antidiabetic Activity of the chemical components from Bignai Leaves (Antidesma bunius). Research Study (General Science Curriculum), Science High School, College of Education, Cavite State University, Indang, Cavite, April 2014. Adviser: Mrs. Agnes Alimboyoguen

This study entitled “Isolation and Antidiabetic activity of the chemical components from Bignai Leaves (Anfidesma bunius)” was conducted to: determine the physical properties of isolates from bignai in terms of color, odor, texture, and solubility; determine the phytochemical components from bignai and; test the antidiabetic activities of each component present in bignai.

The isolates were obtained using column chromatography and their physical properties were evaluated in terms of odor, color, texture and solubility. Phytochemical analysis was also determined by identifying the presence of glycosides, saponins, flavonoids and terpenoids from the bignai isolates. Furthermore, the antidiabetic activity was tested using the oral glucose tolerance test on the laboratory rats.

The findings revealed brown color from the 30%, 60%, 90% and 100% acetone. The results also revealed that all isolates have a pungent odor and greasy texture. The results showed that the isolate from 100% DCM was found insoluble in both distilled water and petroleum ether but soluble in both methanol and ethanol. Meanwhile, the 30%, 60%, 90% and 100% acetone were found insoluble in distilled water but soluble in petroleum ether, methanol, and ethanol.

The results revealed that the isolates from DCM, 30%, 60% and 100% acetone have glycosides. On the other hand, the presence of saponins was found only from 60%, 90% and 100% acetone. The presence of flavonoid was found in 30%, 60% and 100% acetone. In addition, terpenoids was identified in DCM, 30%, 60% and 100% acetone. The mean zone of glucose reduction of the glucose reduction of the DCM was 22.60, the 30% acetone was 20.40. the 60% acetone was 17.40, the 90% acetone was 8.80, and the 100% acetone was 10.60.

Based on the results, the isolates showed glucose reduction but exhibited low antidiabetic activity.

Submitted copy to the University Library. 04/24/2014 RS-701