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Morphological and physiological screening of rice (Oryza sativa L.) for salinity tolerance at seedling stage using the rice diversity panel / by Alfredo V. Alcantara, Jr.

By:

Alcantara, Alfred V. Jr [author]

Contributor(s):

de Ocampo, Marjorie P [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xiv, 76 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

633.18 Al1 2015

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Arts and Science (CAS)

Abstract: ALCANTARA, ALFREDO, JR. V. Morphological and Physiological Screening of Rice (Oryza sativa L.) for Salinity Tolerance at Seedling Stage Using the Rice Diversity Panel. Undergraduate Thesis. Bachelor of Science in Biology Major in General Biology, College of Arts and Sciences, Cavite State University, Indang, Cavite. April 2015. Adviser: Dr. Miriam D. Baltazar; Co-adviser: Ms. Marjorie P. de Ocampo. The study generally aims to screen rice (Oryza sativa L.) accessions under the Rice Diversity Panel tolerant to salinity at seedling stage using morphological and physiological parameters. Specifically it aims to compare the growth and physiological responses of different rice varieties to salinity stress; determine which varieties are considered tolerant or sensitive based on different parameters measured; and assess which physiological characters best contribute to salinity tolerance. Screening method utilized for morphological scoring was based on modified Standard Evaluation System (SES) scores of visual salt injury at seedling stage developed by IRRI. This involves exposing Yoshida’s solution as a basal medium supplemented with sodium chloride (NaCl) to obtain a final electrical conductivity (EC) of 12 dS m’. Physiological screenings done were vigour test, biomass test, chlorophyll content determination and sodium-potassium ratio measurement. Based on morphological screening, out of 324 rice accessions assessed, 87 (27%) were classified as highly tolerant to saline stress, where M 202, MINGHUI 63, ECIA76-S89-1, MING HUI showed the highest degree of tolerance. There were 195 (60%) accessions considered as tolerant, while 36 (11%) were moderately tolerant. Only 4 (1%) accessions were classified as susceptible and only 2 accessions (C57-5043 and WC4419) were highly susceptible. Based on statistical analysis, among the physiological parameters used, vigor seedling growth and root biomass do not correlate with the morphological SES scores, while shoot biomass, chlorophyll content and sodium-potassium ratio correlate with the morphological result. These indicate that these physiological parameters must be considered for future studies in developing rice for salinity tolerance.

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	633.18 Al1 2015 (Browse shelf(Opens below))		Link to resource	Room use only	T-6164		00010138

Thesis (Bachelor of Science in Biology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

ALCANTARA, ALFREDO, JR. V. Morphological and Physiological Screening of Rice (Oryza sativa L.) for Salinity Tolerance at Seedling Stage Using the Rice Diversity Panel. Undergraduate Thesis. Bachelor of Science in Biology Major in General Biology, College of Arts and Sciences, Cavite State University, Indang, Cavite. April 2015. Adviser: Dr. Miriam D. Baltazar; Co-adviser: Ms. Marjorie P. de Ocampo.

The study generally aims to screen rice (Oryza sativa L.) accessions under the Rice Diversity Panel tolerant to salinity at seedling stage using morphological and physiological parameters. Specifically it aims to compare the growth and physiological responses of different rice varieties to salinity stress; determine which varieties are considered tolerant or sensitive based on different parameters measured; and assess which physiological characters best contribute to salinity tolerance.

Screening method utilized for morphological scoring was based on modified Standard Evaluation System (SES) scores of visual salt injury at seedling stage developed by IRRI. This involves exposing Yoshida’s solution as a basal medium supplemented with sodium chloride (NaCl) to obtain a final electrical conductivity (EC) of 12 dS m’. Physiological screenings done were vigour test, biomass test, chlorophyll content determination and sodium-potassium ratio measurement.

Based on morphological screening, out of 324 rice accessions assessed, 87 (27%) were classified as highly tolerant to saline stress, where M 202, MINGHUI 63, ECIA76-S89-1, MING HUI showed the highest degree of tolerance. There were 195 (60%) accessions considered as tolerant, while 36 (11%) were moderately tolerant. Only 4 (1%) accessions were classified as susceptible and only 2 accessions (C57-5043 and WC4419) were highly susceptible.

Based on statistical analysis, among the physiological parameters used, vigor seedling growth and root biomass do not correlate with the morphological SES scores, while shoot biomass, chlorophyll content and sodium-potassium ratio correlate with the morphological result. These indicate that these physiological parameters must be considered for future studies in developing rice for salinity tolerance.

Submitted copy to the University Library. 02/14/2017 T-6164