Isolation and identification of potentially pathogenic bacteria from street vended fish ball sauce in Public Market of Trece Martires City, Cavite / by Jeizelle Deina C. Angcaya, Baby Gael T. Lacanaria and Andrea Cassandra P. Llantos.

Research Study (Bachelor of Science in Medical Technology) Cavite State University.

Includes bibliographical references.

College of Nursing (CON), Department of Medical Technology

ANGCAYA, JEIZELLE DEINA C., LACANARIA, BABY GAEL T., and LLANTOS, ANDREA CASSANDRA P. Isolation and identification of potentially pathogenic bacteria from street vended fish ball sauce in public market of Trece Martires City_ Cavite. Undergraduate Research Manuscript, Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite. October 2018. Adviser: Charmaine V. Rosales, RMT.
This study was conducted to isolate and identify potentially pathogenic bacteria from street vended fish ball sauce sold in the public market of Trece Martires City, Cavite. Specifically, the study aimed to isolate microorganisms that may be present from the street vended fish ball sauce that will be collected; identify the potential pathogens from the isolated samples through colonial morphology and biochemical testing; determine which bacteria has the highest frequency. Descriptive design method was used. In data analysis, the study used descriptive statistics. Twenty different street vended fish ball sauce samples were collected from different areas in the public market of Trece Martires City, Cavite. Four types of selective and differential media were used for isolation: Mac-Conkey agar (MAC), Thiosulfate-Citrate-Bile Salts-Sucrose agar (TCBS agar), Salmonella- Shigella agar (SS agar) and Eosin-Methylene Blue (EMB). Serial dilution of each sauce sample was performed then samples were cultured on selective media plates by pour plate technique. Biochemical tests were performed to identify differences in the biochemical properties of different bacteria. The result shows that all the 20 different street vended fish ball sauce samples had grown gram negative bacteria. Eleven (11) samples yielded lactose fermenting bacteria - Vibrio vulnificus, Escherichia coli, Enterobacter spp. and nine (9) samples yielded non- lactose fermenting bacteria Salmonella, Pseudomonas, Vibrio parahemolyticus, Proteus vulgaris, and Shigella..
Based on the results gathered, the researchers hereby concluded that the street vended fish ball sauce in the public market of Trece Martires City, Cavite contains potentially pathogenic bacteria that can pose health hazard to the consumers and to the vendor himself/herself. The majority of potentially pathogenic bacteria isolated were included in the family of Entero bacteriaceae which is known to cause diseases especially gastrointestinal diseases. Among the potentially pathogenic bacteria isolated and identified, the species with the highest frequency were Vibrio vulnificus and Escherichia coli were identical and been present four times on different samples.
Based on the conclusions above, the researchers recommended to conduct investigative studies to determine the occurrence of related diseases and to determine an effective way to lessen the load of pathogenic organism in the street vended fish ball sauce. Studies should also be conducted to determine the infective dose of the potentially pathogenic bacteria isolated from street vended fish ball sauce. Concerned authorities from Trece Martires City, Cavite should properly inform the community of the potential pathogenic bacteria that may acquire from the street vended fish ball sauce to prevent the occurrence of disease.

Submitted to the University Library May 22, 2019 RS-842