Molecular detection of Rickettsia spp. in cattle from selected farms in Cavite using polymerase chain reaction / by Lena C. Beraña.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2019.Description: vii, 46 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.2  B45 2019
Online resources: Production credits:
  • College of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health
Abstract: BERANA, LENA C. Molecular Detection of Rickettsia spp., in Cattle from Selected Farms in Cavite using Polymerase Chain Reaction. Undergraduate Thesis Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. June 2019. Adviser: Dr. Cherry R. Alvarez The study was conducted to detect the presence of Rickettsia spp. among cattle from selected farms in Cavite. One hundred cattle were sampled from different municipalities in Cavite through blood collection from the ear vein. Then after, the DNA was extracted using a commercially available DNA/RNA extraction kit with the mammalian actin gene serving as the internal control. The detection rate was correlated to different risk factors such as sex, age, type of operation, breed, topography of farm and acaricidal treatment. The result of the study suggested that Rickettsia spp. is not present in the areas of collection as shown in the PCR results.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.2 B45 2019 (Browse shelf(Opens below)) Link to resource Room use only T-8160 00079315

Thesis (Doctor of Veterinary Medicine) Cavite State University.

Includes bibliographical references.

College of Veterinary Medicine and Biological Science (CVMBS), Department of Clinical and Population Health

BERANA, LENA C. Molecular Detection of Rickettsia spp., in Cattle from Selected Farms in Cavite using Polymerase Chain Reaction. Undergraduate Thesis Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. June 2019. Adviser: Dr. Cherry R. Alvarez
The study was conducted to detect the presence of Rickettsia spp. among cattle from selected farms in Cavite. One hundred cattle were sampled from different municipalities in Cavite through blood collection from the ear vein. Then after, the DNA was extracted using a commercially available DNA/RNA extraction kit with the mammalian actin gene serving as the internal control. The detection rate was correlated to different risk factors such as sex, age, type of operation, breed, topography of farm and acaricidal treatment. The result of the study suggested that Rickettsia spp. is not present in the areas of collection as shown in the PCR results.

Submitted to the University Library June 24, 2019 T-8160

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