Isolation, characterization and antibiotic sensitivity testing of Haemophilus paragallinarum in chickens in a dressing plant in Silang, Cavite / by Richard V. Pillerva.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2007. Cavite State University- Main Campus,Description: xiv, 62 pages : 28 cm. illustrationsContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.513  P64 2007
Online resources: Production credits:
  • College of Agriculture, Food, Environment and Natural Resources (CAFENR)
Abstract: PILLERVA, RICHARD V. April 2007. Cavite State University Indang, Cavite. Isolation, Characterization and Antibiotic Sensitivity Testing of Haemophilus paragallinarum in Chickens in a Dressing Plant in Silang, Cavite. Adviser: Ma. Cynthia N. Rundina- dela Cruz, DVM, MS. This study was conducted to isolate and characterise Haentophilltss paragallinanan in nasal swabs of 100 broiler chickens in a dressing plant in Silang, Cavite, perform antibiotic sensitivity testing of the isolates using selected antimicrobials and determine the prevalence of the organism. Ninety five (95) pinpoint, iridescent and semi opaque colonies were isolated from Chocolate Agar indicating presumptive Haetnophilus paragallinarum organisms. Morphologically, 66 isolates were found to be gram-negative slender rods. Upon biochemical testing, 14 isolates were found to be negative on the following tests: catalase, indole, urease and gelatin liquefaction. The isolates were also oxidase positive and nitrate positive, did not produce hydrogen sulphide but two isolates fermented glucose and 1 isolate maltose without gas formation which are all typical of Haemophilus paragallinarum organisms. The 14 isolates came from four different birds and so representative isolates from each bird were tested for antibiotic sensitivity. All four isolates were found to be susceptible to the following antibiotics: ciprofloxacin, colistin, doxycycline, streptomycin and trimethoprim-sulfisoxazole. However, the isolates were found to be resistant to erythromycin and fosfomycin whereas, intermediate result was obtained with amoxicillin. The prevalence of Haentophihts paragallinanan in broiler carcasses in a dressing plant in Silang, Cavite was therefore found to be 15% and this suggests the potential role of the organism in causing disease in broiler chickens which could lead to economic losses in the poultry industry.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.513 P64 2007 (Browse shelf(Opens below)) Link to resource Room use only T-3469 00007311

Thesis (Doctor of Veterinary Medicine) Cavite State University.

Includes bibliographical references.

College of Agriculture, Food, Environment and Natural Resources (CAFENR)

PILLERVA, RICHARD V. April 2007. Cavite State University Indang, Cavite. Isolation, Characterization and Antibiotic Sensitivity Testing of Haemophilus paragallinarum in Chickens in a Dressing Plant in Silang, Cavite. Adviser: Ma. Cynthia N. Rundina- dela Cruz, DVM, MS.
This study was conducted to isolate and characterise Haentophilltss paragallinanan in nasal swabs of 100 broiler chickens in a dressing plant in Silang, Cavite, perform antibiotic sensitivity testing of the isolates using selected antimicrobials and determine the prevalence of the organism. Ninety five (95) pinpoint, iridescent and semi opaque colonies were isolated from Chocolate Agar indicating presumptive Haetnophilus paragallinarum organisms. Morphologically, 66 isolates were found to be gram-negative slender rods. Upon biochemical testing, 14 isolates were found to be negative on the following tests: catalase, indole, urease and gelatin liquefaction. The isolates were also oxidase positive and nitrate positive, did not produce hydrogen sulphide but two isolates fermented glucose and 1 isolate maltose without gas formation which are all typical of Haemophilus paragallinarum organisms. The 14 isolates came from four different birds and so representative isolates from each bird were tested for antibiotic sensitivity. All four isolates were found to be susceptible to the following antibiotics: ciprofloxacin, colistin, doxycycline, streptomycin and trimethoprim-sulfisoxazole. However, the isolates were found to be resistant to erythromycin and fosfomycin whereas, intermediate result was obtained with amoxicillin. The prevalence of Haentophihts paragallinanan in broiler carcasses in a dressing plant in Silang, Cavite was therefore found to be 15% and this suggests the potential role of the organism in causing disease in broiler chickens which could lead to economic losses in the poultry
industry.

Submitted to the University Library 04-10-2007 T-3469

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