Antibiotic sensitivity profile of Salmonella spp. isolates in the Ileoceca of chicken (Gallus gallus domesticus, Linn) from a dressing plant at Trece Martires City, Cavite / by Neleth A. dela Cruz.
Material type:
TextLanguage: English Publication details: Indang, Cavite : 2008. Cavite State University- Main Campus,Description: xvi, 66 pages : 28 cm. illustrationsContent type: - text
- unmediated
- volume
- 636.513 D37 2008
- College of Veterinary Medicine and Biomedical Sciences (VETMET)
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Theses / Manuscripts
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Ladislao N. Diwa Memorial Library Theses Section | Non-fiction | 636.513 D37 2008 (Browse shelf(Opens below)) | Link to resource | Room use only | T-3738 | 00007500 |
Thesis (Doctor of Veterinary Medicine) Cavite State University
Includes bibliographical references.
College of Veterinary Medicine and Biomedical Sciences (VETMET)
DELA CRUZ, NELETH A. Cavite State University, Indang, Cavite. April 2008. Antibiotic Sensitivity Profile of Salmonella spp Isolates in the Ileocecal of Chicken (Gallus gallus domesticus, Linn) from a Dressing Plant at Trece Martires City, Cavite. Undergraduate Thesis. Doctor of Veterinary Medicine. Adviser: Ma. Cynthia R. dela Cruz, DVM, MS.
This study was done to determine the antibiotic sensitivity profile and prevalence of Salmonella spp. isolates from the ileocecal of chickens in a dressing plant in Trece Martires City, Cavite. All of the 100 ileocecal samples were subjected to cultivation in Xylose Lysine Desoxycholate agar or XLD (Difco®) plate. One hundred ninety-five presumptive Salmonella spp. were isolated as indicated by red colonies with or without black centers. These presumptive colonies were further characterized morphologically and biochemically. One hundred twenty-three isolates were gram-negative and appears red and rod-shaped organisms. Biochemically, the 4 isolates produced the following reactions: Oxidase-negative (-), Alkaline slant/Acid butt with gas production (K/A, gas) in Triple Sugar Iron (TSI) Agar, with hydrogen sulfide production, indole negative (-) and motile in Sulfide Indole Motility (SIM) Medium, Methyl red test positive (+), Voges Proskauer test negative (-), Citrate utilization test positive (+), Gelatin utilization test negative (-), Nitrate reduction test positive (+) and Urease test negative (-). Sugar utilization tests revealed that 4 isolates fermented glucose and maltose while only 2 of the 4 isolates did not ferment lactose and sucrose. These 2 isolates were considered as the presumptive Salmonella spp and were further tested serologically using the polyvalent 0 and Vi antisera. The results of the study showed that the prevalence rate of Salmonella spp. from a dressing plant at Trece Martirez City, Cavite was 2%. These findings indicate that some chickens processed in the dressing plant in Cavite harbored Salmonella spp. in their gut. The two Salmonella spp. isolates have undergone antibiotic sensitivity testing using selected antibiotics and the results where as follows: Isolates 79C and 81C were resistant to lincomycin but susceptible to cephalotin, fosfomycin, gentamicin, norfloxacin, tetracycline, and nitrofurantoin. However, 79C isolate was resistant to ampicilllin and trimethoprim- sulfamethoxazole in contrast to 81C which was susceptible. The results of antibiotic sensitivity testing with ampicillin and trimethoprim-sulfamethoxazole is inconclusive because of different reactions on the 2 isolates in the drugs used.
Submitted to the University Library 04-03-2008 T-3738