Antibiotic sensitivity profile of Salmonella spp. isolates in carcasses of chicken (Gallus gallus domesticus) from selected wet markets in Cavite / by Verna S. Fojas.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2008. Cavite State University- Main Campus,Description: xv, 74 pages : 28 cm. illustrationsContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.513  F69 2008
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: FOJAS, VERNA S., Cavite State University, Indang, Cavite, April 2008. Antibiotic Sensitivity Profile of Salmonella spp. Isolated in Carcasses of Chicken (Gallus gallus domesticus, Linn) from Selected Wet Markets in Cavite. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. Adviser: Heidee E Arapol, DVM. The study was conducted to determine the antibiotic sensitivity profile of Salmonella spp. isolates from the carcasses of 100 dressed chickens from selected wet markets in Cavite. One hundred and twenty-five colonies from 100 chicken carcasses were isolated from Xylose Lysine Desoxycholate or XLD (Difco®) agar. Morphological characterization showed that 118 of 125 isolates were gram negative, rod shaped organisms. Further characterization was done to the isolates using biochemical tests and revealed the typical Salmonella spp., reactions which are oxidase negative, alkaline slant and acid butt with hydrogen sulfide and gas formation on Triple Sugar Iron or TSI, produces hydrogen sulfide, negative result for indole and motile on Sulfide Indole Motility or SIM, Methyl Red test positive and Voges-Proskauer test negative, Citrate and Nitrate Utilization positive and yield a negative result on Urease and Gelatinase test. The isolates also fermented glucose and maltose but not fermentative in sucrose. Serological characterization was done on the isolates using Somatic 0 and Vi and Vi antisera and seven isolates were identified as Salmonella spp. All seven isolates were found to be susceptible to fosfomycin, ampicillin, nitrofurantoin, norfloxacin, trimethoprim-sulphamethoxazole and ciprofloxacin and were resistant to lincomycin and gentamicin. Intermediate results were obtained to doxycycline and tetracycline. The prevalence rate of Salmonella spp. in the carcasses of chicken from selected wet markets in Cavite was found to be 7%. Hence, proper handling, processing and storage of chicken carcasses must be practiced to help ensure the safety of the carcass as well ensure that the public do not get infected by the organism. In addition, the discriminate use of antimicrobials in food-producing animals must be monitored to guarantee the continued safety and efficacy of veterinary antimicrobials.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.513 F69 2008 (Browse shelf(Opens below)) Link to resource Room use only T-3732 00007501

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

FOJAS, VERNA S., Cavite State University, Indang, Cavite, April 2008. Antibiotic Sensitivity Profile of Salmonella spp. Isolated in Carcasses of Chicken (Gallus gallus domesticus, Linn) from Selected Wet Markets in Cavite. Doctor of Veterinary Medicine, Cavite State University, Indang, Cavite. Adviser: Heidee E Arapol, DVM.
The study was conducted to determine the antibiotic sensitivity profile of Salmonella spp. isolates from the carcasses of 100 dressed chickens from selected wet markets in Cavite. One hundred and twenty-five colonies from 100 chicken carcasses were isolated from Xylose Lysine Desoxycholate or XLD (Difco®) agar. Morphological characterization showed that 118 of 125 isolates were gram negative, rod shaped organisms. Further characterization was done to the isolates using biochemical tests and revealed the typical Salmonella spp., reactions which are oxidase negative, alkaline slant and acid butt with hydrogen sulfide and gas formation on Triple Sugar Iron or TSI, produces hydrogen sulfide, negative result for indole and motile on Sulfide Indole Motility or SIM, Methyl Red test positive and Voges-Proskauer test negative, Citrate and Nitrate Utilization positive and yield a negative result on Urease and Gelatinase test. The isolates also fermented glucose and maltose but not fermentative in sucrose. Serological characterization was done on the isolates using Somatic 0 and Vi and Vi antisera and seven isolates were identified as Salmonella spp. All seven isolates were found to be susceptible to fosfomycin, ampicillin, nitrofurantoin, norfloxacin, trimethoprim-sulphamethoxazole and ciprofloxacin and were resistant to lincomycin and gentamicin. Intermediate results were obtained to doxycycline and tetracycline.
The prevalence rate of Salmonella spp. in the carcasses of chicken from selected wet markets in Cavite was found to be 7%. Hence, proper handling, processing and storage of chicken carcasses must be practiced to help ensure the safety of the carcass as well ensure that the public do not get infected by the organism. In addition, the discriminate use of antimicrobials in food-producing animals must be monitored to guarantee the continued safety and efficacy of veterinary antimicrobials.

Submitted to the University Library 04-03-2008 T-3732

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