Identification of bacteria causing pneumonia in air conditioning units in tertiary hospital in Cavite / by Arianne Rose A. Lim and Charles Angelo P. Villa.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : Cavite State University- Main Campus, 2015.Description: xiv, 53 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 579.3  L62 2014
Online resources: Production credits:
  • College of Nursing (CON)
Abstract: LIM, ARIANNE ROSE A., AND VILLA, CHARLES ANGELO P., Identification of Bacteria Causing Pneumonia in Air Conditioning Units in a Tertiary Hospital in Cavite. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, April 2014. Adviser: Fedelyn P. Estrella, RMT, MPH This study was conducted from May to June 2013 at the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. The study aimed to identify the bacteria causing pneumonia in air conditioning units in a tertiary hospital in Cavite. The samples were cultured in 4 different culture media and subjected to different biochemical tests to identify the isolated organisms. Cetrimide test was done to identify Pseudomonas aeruginosa. Thirteen isolates were found out as Pseudomonas aeruginosa. Haemophilus influenzae were identified by X and V factor requirement and satellitism test. In the X and V factor requirement, 100 % or 18 samples had a positive result indicating that these samples were Haemophilus species. On the second test, 15 samples were positive as Haemophilus influenzae. Optochin susceptibility test was done to identify Streptococcus pneumoniae. Out of the 20 samples, only 2 had a zone of inhibition of 14 mm in diameter, this denotes susceptibility to optochin and was Streptococcus pneumoniae. The remaining samples were all resistant having a zone of inhibition lower than 14 mm. Triple sugar iron (TSI) test was performed to identify Klebsiella pneumoniae. The results showed that of all the samples, there were no positive for Klebsiella pneumoniae. Based on the results gathered, the isolated organism with the highest incidence was Haemophilus influenzae and was isolated and identified in 15 samples that were gathered in the private rooms and wards. It was followed by Pseudomonas aeruginosa which were identified among the 13 samples and Streptococcus pneumoniae were identified in 2 samples based on the results of the tests.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 579.3 L62 2014 (Browse shelf(Opens below)) Link to resource Room use only T-5626 00009138

Thesis ( BS Medical Technology ) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

LIM, ARIANNE ROSE A., AND VILLA, CHARLES ANGELO P., Identification of Bacteria Causing Pneumonia in Air Conditioning Units in a Tertiary Hospital in Cavite. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, April 2014. Adviser: Fedelyn P. Estrella, RMT, MPH
This study was conducted from May to June 2013 at the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. The study aimed to identify the bacteria causing pneumonia in air conditioning units in a tertiary hospital in Cavite. The samples were cultured in 4 different culture media and subjected to different biochemical tests to identify the isolated organisms. Cetrimide test was done to identify Pseudomonas aeruginosa. Thirteen isolates were found out as Pseudomonas aeruginosa. Haemophilus influenzae were identified by X and V factor requirement and satellitism test. In the X and V factor requirement, 100 % or 18 samples had a positive result indicating that these samples were Haemophilus species. On the second test, 15 samples were positive as Haemophilus influenzae. Optochin susceptibility test was done to identify Streptococcus pneumoniae. Out of the 20 samples, only 2 had a zone of inhibition of 14 mm in diameter, this denotes susceptibility to optochin and was Streptococcus pneumoniae. The remaining samples were all resistant having a zone of inhibition lower than 14 mm. Triple sugar iron (TSI) test was performed to identify Klebsiella pneumoniae. The results showed that of all the samples, there were no positive for Klebsiella pneumoniae.
Based on the results gathered, the isolated organism with the highest incidence was Haemophilus influenzae and was isolated and identified in 15 samples that were gathered in the private rooms and wards. It was followed by Pseudomonas aeruginosa which were identified among the 13 samples and Streptococcus pneumoniae were identified in 2 samples based on the results of the tests.

Submitted copy to the University Library. 01/30/2015 T-5626

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