Production of germicidal soap with guava (Psidium guajava) leaves extract / by Neacail F. Alvaran and Pol Andrei A. Romanes.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: viii, 40 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 614.4  Al8 2015
Online resources: Production credits:
  • Science High School, College of Education (CED)
Abstract: ALVARAN, NEACAIL F. AND ROMANES, POL ANDREI A. Production of Germicidal Soap from Guava Leaves Extract. Research Study, Science High School, College of Education, Cavite State University, Indang, Cavite. April 2015. Adviser Engr. Momeda Callao. The study “Production of Germicidal Soap from Guava Leaves Extract” was conducted in Physical Sciences Department, Cavite State University and Biological Sciences Department, Cavite State University from November 2014 to January 2015. Generally, it aimed to determine the kind of guava leaves extract (young, matured and its combination) that produced the best germicidal soap. Specifically, it aimed to: determine the treatment that exhibited presence of zone of inhibition in each bacteria: determine the antibacterial activity of the germicidal soap from guava leaves extract against Staphylococcus aureus, Bacillus subtilis and Escherichia coli: determine the treatment that will produced the best germicidal soap from guava leaves extract; and compare the zone of inhibition produced by germicidal soap from guava leaves extract to the commercially available germicidal soap. The treatments used in the study were: T; or germicidal soap with young guava leaves extract, T; or germicidal soap with the combination of young and matured guava leaves extract, T3 or germicidal soap with matured guava leaves extract and To or the commercially available germicidal soap “Bioderm”. Guava germicidal soaps were made in Physical Sciences Department, Cavite State University and were tested against Staphylococcus aureus, Bacillus subtilis and Escherichia coli in Biological Sciences Department, Cavite State University. All soaps exhibited zones of inhibition on cultures of S. aureus and B. subtilis but did not show zone of inhibition on the cultures of E. coli. Laboratory experiments showed that the germicidal soap from guava leaves extract have minimum activity against pathogens S. aureus and B. subtilis while showed no effect on E. coli. Also it indicated that Treatment 3 was the best among the three treatments in terms of inhibiting the growth of S. aureus and B. subtilis. In comparison with the zone of inhibition of the control treatment “Bioderm”, the germicidal soap from mature guava leaves extract was better than that of the “Bioderm”’. Treatment 3 (matured guava leaves) gave the highest antibacterial activity with a mean zone of inhibition of 1.55cm. Thus, Treatment 3 produced the best germicidal soap among the three treatments. Treatment 3 was also comparable to the commercially available germicidal soap “Bioderm” in terms of its antibacterial activity with a zone of inhibition of 1.55cm as compared to 1.70cm of the commercial soap ”Bioderm”, and a zone of inhibition of 2.35cm as compared to 1.97cm, for S. aureus and B.subtilis, respectively.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 614.4 Al8 2015 (Browse shelf(Opens below)) Link to resource Room use only RS-726 00009318

Research Study (Science High School) Cavite State University

Includes bibliographical references.

Science High School, College of Education (CED)

ALVARAN, NEACAIL F. AND ROMANES, POL ANDREI A. Production of Germicidal Soap from Guava Leaves Extract. Research Study, Science High School, College of
Education, Cavite State University, Indang, Cavite. April 2015. Adviser Engr. Momeda Callao.

The study “Production of Germicidal Soap from Guava Leaves Extract” was conducted in Physical Sciences Department, Cavite State University and Biological Sciences Department, Cavite State University from November 2014 to January 2015. Generally, it aimed to determine the kind of guava leaves extract (young, matured and its combination) that produced the best germicidal soap. Specifically, it aimed to: determine the treatment that exhibited presence of zone of inhibition in each bacteria: determine the antibacterial activity of the germicidal soap from guava leaves extract against Staphylococcus aureus, Bacillus subtilis and Escherichia coli: determine the treatment that will produced the best germicidal soap from guava leaves extract; and compare the zone of inhibition produced by germicidal soap from guava leaves extract to the commercially available germicidal soap.

The treatments used in the study were: T; or germicidal soap with young guava leaves extract, T; or germicidal soap with the combination of young and matured guava leaves extract, T3 or germicidal soap with matured guava leaves extract and To or the commercially available germicidal soap “Bioderm”.

Guava germicidal soaps were made in Physical Sciences Department, Cavite State University and were tested against Staphylococcus aureus, Bacillus subtilis and Escherichia coli in Biological Sciences Department, Cavite State University. All soaps exhibited zones of inhibition on cultures of S. aureus and B. subtilis but did not show zone of inhibition on the cultures of E. coli.

Laboratory experiments showed that the germicidal soap from guava leaves extract have minimum activity against pathogens S. aureus and B. subtilis while showed no effect on E. coli. Also it indicated that Treatment 3 was the best among the three treatments in terms of inhibiting the growth of S. aureus and B. subtilis. In comparison with the zone of inhibition of the control treatment “Bioderm”, the germicidal soap from mature guava leaves extract was better than that of the “Bioderm”’.

Treatment 3 (matured guava leaves) gave the highest antibacterial activity with a mean zone of inhibition of 1.55cm. Thus, Treatment 3 produced the best germicidal soap among the three treatments.

Treatment 3 was also comparable to the commercially available germicidal soap
“Bioderm” in terms of its antibacterial activity with a zone of inhibition of 1.55cm as compared
to 1.70cm of the commercial soap ”Bioderm”, and a zone of inhibition of 2.35cm as compared to
1.97cm, for S. aureus and B.subtilis, respectively.

Submitted copy to the University Library. 08/18/2015 RS-726

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