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Effect of Citrus microcarpa essential oil against Entamoeba histolytica isolate from plant, water and human sources in Indang, Cavite / by Aira Joy C. Mendoza, Jillian U. Samaniego and Chlorish Ann E. Villete.

By:

Mendoza, Aira Joy C [author]

Contributor(s):

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xvi, 96 pages : 28 cm. illustrationsContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

614.4 M52 2016

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Nursing (CON)

Abstract: MENDOZA, AIRA JOY, SAMANIEGO, JILLIAN, VILLETE, CHLORISH ANN. Effect of Citrus microcarpa Essential Oil Against Entamoeba histolytica Isolates from Plant, Water and Human Sources in Indang, Cavite Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite. October 2016. Adviser: Lenila A. de Vera, RN, IvWH, MAN. Co-adviser: Fiordeliza A. de Guzman, RMT, MAT. This study, "Effect of Citrus microcarpa Essential Oil Against Entamoeba histolytica Isolates from Plant, Water and Human Sources in Indang, Cavite," was conducted at the Department of Medical Technology, College of Nursing from March 2016 to August 2016. The study generally aimed to determine the effect of Citrus microcarpa essential oil against Entamoeba histolytica isolates from plant, water and human sources in Indang, Cavite. Specifically, the objectives of the study were to: identify the sources that were positive with Entamoeba histolytica: (a) plant sources, (b) water sources and (c) human sources; determine which of the Entamoeba histolytica isolates from plant, water and human sources were more susceptible to the Citrus microcarpa essential oil; and determine which among the different concentrations of Citrus microcarpa essential oil had the most antiamoebic effect. Experimental research approach was used in this study to identify the effect of Citrus microcarpa (Calamansi) essential oil against Entamoeba histolytica isolates from plant, water and human sources in Indang, Cavite. Water samples were collected from the university water system and the collected rainfall water from the Farmer's Training Center and Techno Demo Farm (SAKA). Soil samples were collected from the different areas of SAKA: (a) open field, (b) module field vii and (c) urban field where agricultural products were cultivated. The samples from the human sources were collected from the in-patients of Maviz Beatriz Maternity Clinic who were positive with Entamoeba histolytica. Calamansi were collected from Tambo Malaki, Indang, Cavite. The essential oil was extracted from Citrus microcarpa (Calamansi) peel using steam distillation process. The soil and water samples were inoculated and cultured in Locke Egg Medium. After incubation, the samples were viewed under the microscope to determine the presence of Entamoeba histolytica. Results showed that the plant and water sources in Cavite State University main Campus were negative of Entamoeba histolytica. The soil samples from the SAKA (urban, module, open) yielded negative when tested for the presence of Entamoeba histolytica. The water samples from SAKA and Physical Plant Section department also resulted negative from Entamoeba histolytica. The 100 percent concentration of Citrus microcarpa essential oil had the highest mean of dead trophozoites with the value of eight while the 50 percent concentration had the lowest mean of dead trophozoites with the value of 4.67. The 75 percent concentration had the highest mean of dead cysts with the value of eight while the 25 percent concentration had the lowest mean of dead cysts with the value of five. Although the 75 percent concentration had the higher mean, the 100 percent concentration had the higher average of dead cysts. Therefore, Citrus microcarpa essential oil had an antiamoebic effect on Entamoeba histolytica.

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Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	614.4 M52 2016 (Browse shelf(Opens below))		Link to resource	Room use only	T-6616		00010907

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

MENDOZA, AIRA JOY, SAMANIEGO, JILLIAN, VILLETE, CHLORISH ANN.
Effect of Citrus microcarpa Essential Oil Against Entamoeba histolytica Isolates from Plant, Water and Human Sources in Indang, Cavite Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite. October 2016. Adviser: Lenila A. de Vera, RN, IvWH, MAN. Co-adviser: Fiordeliza A. de Guzman, RMT, MAT.
This study, "Effect of Citrus microcarpa Essential Oil Against Entamoeba histolytica Isolates from Plant, Water and Human Sources in Indang, Cavite," was conducted at the Department of Medical Technology, College of Nursing from March 2016 to August 2016. The study generally aimed to determine the effect of Citrus microcarpa essential oil against Entamoeba histolytica isolates from plant, water and human sources in Indang, Cavite. Specifically, the objectives of the study were to: identify the sources that were positive with Entamoeba histolytica: (a) plant sources, (b) water sources and (c) human sources; determine which of the Entamoeba histolytica isolates from plant, water and human sources were more susceptible to the Citrus microcarpa essential oil; and determine which among the different concentrations of Citrus microcarpa essential oil had the most antiamoebic effect.
Experimental research approach was used in this study to identify the effect of Citrus microcarpa (Calamansi) essential oil against Entamoeba histolytica isolates from plant, water and human sources in Indang, Cavite.
Water samples were collected from the university water system and the collected rainfall water from the Farmer's Training Center and Techno Demo Farm (SAKA). Soil samples were collected from the different areas of SAKA: (a) open field, (b) module field
vii
and (c) urban field where agricultural products were cultivated. The samples from the human sources were collected from the in-patients of Maviz Beatriz Maternity Clinic who were positive with Entamoeba histolytica.
Calamansi were collected from Tambo Malaki, Indang, Cavite. The essential oil was extracted from Citrus microcarpa (Calamansi) peel using steam distillation process.
The soil and water samples were inoculated and cultured in Locke Egg Medium. After incubation, the samples were viewed under the microscope to determine the presence of Entamoeba histolytica. Results showed that the plant and water sources in Cavite State University main Campus were negative of Entamoeba histolytica. The soil samples from the SAKA (urban, module, open) yielded negative when tested for the presence of Entamoeba histolytica. The water samples from SAKA and Physical Plant Section department also resulted negative from Entamoeba histolytica.
The 100 percent concentration of Citrus microcarpa essential oil had the highest mean of dead trophozoites with the value of eight while the 50 percent concentration had the lowest mean of dead trophozoites with the value of 4.67. The 75 percent concentration had the highest mean of dead cysts with the value of eight while the 25 percent concentration had the lowest mean of dead cysts with the value of five. Although the 75 percent concentration had the higher mean, the 100 percent concentration had the higher average of dead cysts. Therefore, Citrus microcarpa essential oil had an antiamoebic effect on Entamoeba histolytica.

Submitted copy to the University Library. 05/10/2017 T-6616