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Determination of antimicrobial activity of cape leadwort (Plumbago auriculata) extract / by Trixia Anjane R. Espadilla, Clarence Gaebriel R. Rodriguez and Lelaine Marie G. Saria.

By:

Espadilla, Trixia Anjane R [author]

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Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2015. Cavite State University- Main Campus,Description: xvi, 62 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

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volume

Subject(s):

DDC classification:

579.3 Es6 2015

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Click here to view thesis abstract and table of contents

Production credits:

College of Nursing (CON)

Abstract: ESPADILLA, TRIXIA ANJANE R., RODRIGUEZ, CLARENCE GAEBRIEL R., AND SARIA, LALAINE MARIE G., Determination of Antimicrobial Activity of Cape Leadwort (Plumbago auriculata) Extract. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, November 2015. Adviser: Prof. Ronalyn S. Sanchez, RMT This study was conducted from April to December 2014 at the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. The study aimed to determine the antimicrobial activity of Cape Leadwort (Plumbago auriculata) extract. Dried leaves and roots of P. auriculata were extracted with Soxhlet method using absolute ethanol and obtained both the polar and non-polar extract. Susceptibility test for bacteria was performed using Cup Plate method. Both treatments showed low inhibition with root ethanolic extract having a higher inhibitory activity than leaf ethanolic extract against Enterobacter aerogenes, Serratia marcescens and Staphylococcus aureus. Results from measured diameter zone of inhibition exhibited that root ethanolic extract was more effective than the leaf ethanolic extract, however, EF. aerogenes, S. marcescens and S. aureus were resistant to these ethanolic extracts. Poison-plate method was used to determine the fungicidal activity of P. auriculata extracts against Fusarium oxysporum. Root ethanolic extract exhibited significant growth inhibition of mycelia compared to the negative. P. auriculata leaf ethanolic extract showed lower inhibition. Results revealed higher inhibitory activity of root ethanolic extract compared to the leaf ethanolic extract. The biochemical compound, plumbagin, showed significant inhibitory activity against the tested microorganisms. Plumbagin was more effectively extracted from the roots since the root extract showed greater activity than the leaf extract against all microorganisms. With further studies, Plumbagin from the root extract of Plumbago auriculata may be a promising source of antibacterial and antifungal drug for infectious diseases.

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Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	579.3 Es6 2015 (Browse shelf(Opens below))		Link to resource	Room use only	T-6656		00010948

Thesis (BS Medical Technology) Cavite State University

Includes bibliographical references.

College of Nursing (CON)

ESPADILLA, TRIXIA ANJANE R., RODRIGUEZ, CLARENCE GAEBRIEL R., AND SARIA, LALAINE MARIE G., Determination of Antimicrobial Activity of Cape Leadwort (Plumbago auriculata) Extract. Undergraduate Thesis. Bachelor of Science in Medical Technology, Cavite State University, Indang, Cavite, November 2015.
Adviser: Prof. Ronalyn S. Sanchez, RMT

This study was conducted from April to December 2014 at the Department of Medical Technology, College of Nursing, Cavite State University, Indang, Cavite. The study aimed to determine the antimicrobial activity of Cape Leadwort (Plumbago auriculata) extract. Dried leaves and roots of P. auriculata were extracted with Soxhlet method using absolute ethanol and obtained both the polar and non-polar extract.

Susceptibility test for bacteria was performed using Cup Plate method. Both treatments showed low inhibition with root ethanolic extract having a higher inhibitory
activity than leaf ethanolic extract against Enterobacter aerogenes, Serratia marcescens and Staphylococcus aureus. Results from measured diameter zone of inhibition exhibited that root ethanolic extract was more effective than the leaf ethanolic extract, however, EF. aerogenes, S. marcescens and S. aureus were resistant to these ethanolic extracts.

Poison-plate method was used to determine the fungicidal activity of P. auriculata extracts against Fusarium oxysporum. Root ethanolic extract exhibited significant growth inhibition of mycelia compared to the negative. P. auriculata leaf ethanolic extract showed lower inhibition. Results revealed higher inhibitory activity of root ethanolic extract compared to the leaf ethanolic extract.

The biochemical compound, plumbagin, showed significant inhibitory activity against the tested microorganisms. Plumbagin was more effectively extracted from the roots since the root extract showed greater activity than the leaf extract against all microorganisms. With further studies, Plumbagin from the root extract of Plumbago auriculata may be a promising source of antibacterial and antifungal drug for infectious diseases.

Submitted copy to the University Library. 05/25/2017 T-6656