Detection of mastitis and prevalence of Staphylococcus aureus in raw milk from dairy caracows in selected backyard farms of General Trias, Cavite / by Judeth Paula B. Laig.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2016. Cavite State University- Main Campus,Description: xv, 88 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
Subject(s): DDC classification:
  • 636.2  L14 2016
Online resources: Production credits:
  • College of Veterinary Medicine and Biomedical Sciences (VETMET)
Abstract: LAIG, JUDETH PAULA B. Detection of Mastitis and Prevalence of Staphylococcus aureus in Raw Milk from Dairy Caracows in Selected Backyard Farms of General Trias, Cavite. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2016. Adviser: Dr. Ma. Cynthia R. dela Cruz. The study was conducted from April 2014 to September 2015 in selected backyard farms in General Trias, Cavite to detect mastitis and determine the prevalence of Staphylococcus aureus in raw milk from lactating caracows. A total of 65 out of 70 (92.86%) lactating caracows from 30 backyard farms in 2 barangays were negative in California Mastitis Test (CMT) and the udders appeared normal or given Negative (N) scale. On the other hand, positive CMT is at 7.14% (5/70) and consisted of 4 (5.71%) caracows with subclinical mastitis and 1 (1.42%) caracow with clinical mastitis. Staphylococcus aureus colonies were isolated from 2 out of 70 (2.85%) raw caracow's milk. One isolate came from an animal with subclinical mastitis while the other isolate came from an animal with clinical mastitis. However, 3 (4.28%) of the positive CMT raw milk samples were discovered to be negative for S. aureus. The mastitis in the CMT positive milk can be potentially caused by other microorganisms. Moreover, atypical S. aureus may also be a cause of mastitis but this was not explored because the study was limited to typical S. aureus only. Antibiotic sensitivity testing showed that the isolates were susceptible to cotrimoxazole, clarithromycin, ampicillin, and tetracycline. The isolates showed intermediate susceptibility to streptomycin, while one isolate was susceptible to erythromycin and penicillin G and the other isolate showed intermediate susceptibility to the two drugs. The prevalence of S. aureus in the caracows examined from selected backyard farms in Gen. Trias, Cavite was found to be 2.86% and waslower than published data in dairy cattle in the Philippines. However, to the best of our knowledge this study is the first of its kind in Cavite
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 636.2 L14 2016 (Browse shelf(Opens below)) Link to resource Room use only T-6157 00011017

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

LAIG, JUDETH PAULA B. Detection of Mastitis and Prevalence of Staphylococcus aureus in Raw Milk from Dairy Caracows in Selected Backyard Farms of General Trias, Cavite. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite. April 2016. Adviser: Dr. Ma. Cynthia R. dela Cruz.

The study was conducted from April 2014 to September 2015 in selected backyard farms in General Trias, Cavite to detect mastitis and determine the prevalence of Staphylococcus aureus in raw milk from lactating caracows. A total of 65 out of 70 (92.86%) lactating caracows from 30 backyard farms in 2 barangays were negative in California Mastitis Test (CMT) and the udders appeared normal or given Negative (N) scale. On the other hand, positive CMT is at 7.14% (5/70) and consisted of 4 (5.71%) caracows with subclinical mastitis and 1 (1.42%) caracow with clinical mastitis. Staphylococcus aureus colonies were isolated from 2 out of 70 (2.85%) raw caracow's milk. One isolate came from an animal with subclinical mastitis while the other isolate came from an animal with clinical mastitis.

However, 3 (4.28%) of the positive CMT raw milk samples were discovered to be negative for S. aureus. The mastitis in the CMT positive milk can be potentially caused by other microorganisms. Moreover, atypical S. aureus may also be a cause of mastitis but this was not explored because the study was limited to typical S. aureus only. Antibiotic sensitivity testing showed that the isolates were susceptible to cotrimoxazole, clarithromycin, ampicillin, and tetracycline. The isolates showed intermediate susceptibility to streptomycin, while one isolate was susceptible to erythromycin and penicillin G and the other isolate showed intermediate susceptibility to the two drugs. The prevalence of S. aureus in the caracows examined from selected backyard farms in Gen. Trias, Cavite was found to be 2.86% and waslower than published data in dairy cattle in the Philippines. However, to the best of our
knowledge this study is the first of its kind in Cavite

Submitted copy to the University Library. 02/14/2017 T-6157

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