Enumeration and determination of microorganisms found in CvSU organic soil amendment and its effect on the growth of of romaine lettuce (Lactuca sativa L. var. longifolia) / by Hannah Therese C. Ambas, Patricia Angelus M. Calle and Angelica L. De Guzman.

By: Contributor(s): Material type: TextTextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xiv, 60 pages : illustrations ; 28 cmContent type:
  • text
Media type:
  • unmediated
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Subject(s): DDC classification:
  • 631.4  Am1 2017
Online resources: Production credits:
  • High School
Abstract: AMBAS, HANNAH THERESE C., CALLE, PATRICIA ANGELUS M., and DE GUZMAN, ANGELICA L. Enumeration and Determination of Microorganisms found in CVSU Organic Soil Amendment and its Effect On the Growth of Romaine Lettuce (Lactuca sativa L. var. longifolia). Research Study (General Science Curriculum), Science High School, College of Education, Cavite State University, Indang, Cavite, May 2017. Adviser: Professor Noel A. Sedigo. The study was conducted from November 2016 to March 2017 at the microbiological laboratory of the College of Agriculture, Food and Natural Resources (CAFENR), Science High School Chemistry Laboratory of Cavite State University, Indang Cavite and Halang, Amadeo Cavite to determine the microorganism content and composition of the organic soil amendment produced at the College of Agriculture, Food, Environment, and Natural Resources (CAFENR). Specifically, the study aimed to: (l) determine the different types of microorganisms present in the CvSU organic soil amendment; (2) determine the general composition and count of the different types of microorganisms present in the CvSU organic soil amendment; and (3) determine the effects of using the CvSU organic soil amendment to the growth of lettuce in terms of plant height, number of leaves from transplanting up to harvest, and biomass weight. The first part of the study consisted of determining the microorganisms present in the organic soil amendment following the standard procedures of serial dilution and plating. Viable colonies after incubation period were counted and the total colony forming units (CFU/g) per type of media used were computed. The second part of the research was the planting of lettuce in the different types of soil, namely: CvSU Organic Soil Amendment, ordinary soil, and ordinary soil with commercial fertilizer. The lettuce was planted in black polyethylene plant plastic bags and their growth were observed for two months. The results showed that bacteria are present in the CVSU Organic Soil Amendment the possible identity of which are actinomycetes based on their appearance. The computed amount of bacteria on the soil based on valid counts are 3.75 x 104 CFU/g and 2.46 x 106 CFU/g. The lettuce planted in the ordinary soil (T3) produced the best lettuce in terms of height, number of leaves, and biomass weight. On the other hand, the lettuce planted in the CVSU Organic Soil Amendment is the shortest, last in terms of number of leaves and last in terms of biomass weight.
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Theses / Manuscripts Theses / Manuscripts Ladislao N. Diwa Memorial Library Theses Section Non-fiction 631.4 Am1 2017 (Browse shelf(Opens below)) Link to resource Room use only RS-793 00011220

Research Study (Science High School) Cavite State University

Includes bibliographical references.

High School

AMBAS, HANNAH THERESE C., CALLE, PATRICIA ANGELUS M., and DE
GUZMAN, ANGELICA L. Enumeration and Determination of Microorganisms found in CVSU Organic Soil Amendment and its Effect On the Growth of Romaine Lettuce (Lactuca sativa L. var. longifolia). Research Study (General Science Curriculum), Science High School, College of Education, Cavite State University, Indang, Cavite, May 2017. Adviser: Professor Noel A. Sedigo.
The study was conducted from November 2016 to March 2017 at the microbiological laboratory of the College of Agriculture, Food and Natural Resources (CAFENR), Science High School Chemistry Laboratory of Cavite State University, Indang Cavite and Halang, Amadeo Cavite to determine the microorganism content and composition of the organic soil amendment produced at the College of Agriculture, Food, Environment, and Natural Resources (CAFENR). Specifically, the study aimed to: (l) determine the different types of microorganisms present in the CvSU organic soil amendment; (2) determine the general composition and count of the different types of microorganisms present in the CvSU organic soil amendment; and (3)
determine the effects of using the CvSU organic soil amendment to the growth of lettuce in terms of plant height, number of leaves from transplanting up to harvest, and biomass weight.
The first part of the study consisted of determining the microorganisms present in the organic soil amendment following the standard procedures of serial dilution and plating. Viable colonies after incubation period were counted and the total colony forming units (CFU/g) per type of media used were computed. The second part of the research was the planting of lettuce in the different types of soil, namely: CvSU Organic Soil Amendment, ordinary soil, and ordinary soil with commercial fertilizer. The lettuce was planted in black polyethylene plant plastic bags and their growth were observed for two months.
The results showed that bacteria are present in the CVSU Organic Soil Amendment the possible identity of which are actinomycetes based on their appearance. The computed amount of bacteria on the soil based on valid counts are 3.75 x 104 CFU/g and 2.46 x 106 CFU/g.
The lettuce planted in the ordinary soil (T3) produced the best lettuce in terms of height, number of leaves, and biomass weight. On the other hand, the lettuce planted in the CVSU Organic Soil Amendment is the shortest, last in terms of number of leaves and last in terms of biomass weight.

Submitted copy to the University Library. 07/17/2017 RS-793

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