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In vitro screening of low-density polyethylene biodegrading filamentous fungi isolated form soils in Indang, Cavite / by Yves Roy M. Tibayan.

By:

Tibayan, Yves Roy M [author]

Contributor(s):

Cruzate, Sherine M [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xv, 67 pages : 28 cm. illustrationsContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

668.4 T43 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Arts and Science (CAS)

Abstract: TIBAYAN, YVES ROY M. "In vitro Screening of Low-density Polyethylenebiodegrading Filamentous Fungi Isolated from Soils in Indang, Cavite". Undergraduate Thesis. Bachelor of Science in Biology major in Microbiology. Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Indang, Cavite. May 2017. Adviser: Prof. Sherine M. Cruzate. Low-density polyethylene (LDPE) plastics are substantial wellsprings of pollution as they are recalcitrant and bioinert materials; thus, they are escalating incessantly in the environment. Not only the Philippines, but also the other countries have an immense need to search for efficient and economical approach that would be vastly used to degrade these pollutants. The present work is on the study of LDPE biodegradation by filamentous fungi obtained from different sampling locations in Indang, Cavite. The isolates were first screened for their biodegrading ability based on abundant growths on Mineral Salt Agar (MSA) amended with LDPE powder. Out of 58 isolates, 28 yielded positive results with abundant growths on MSA after a 30-day incubation. Thereafter, they were subjected to in vilro biodegradation assay on Mineral Salt Broth (MSB) containing LDPE plastic strips for 30 days. The 24 filamentous fungal isolates were phenotypically characterized and were identified belonging to four genera, viz. Aspergillus, Penicillium, Paecilomyces and Helminthosporium; four were not identified. The three most promising isolates which exhibited more than 20% biodegradation rates were identified as Aspergillus niger, Paecilomyces sp. and Aspergillus niger with 38.70%, 35.96% and 28.78% rates of biodegradation, correspondingly

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	668.4 T43 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-6879		00011239

Thesis (BS Biology) Cavite State University

Includes bibliographical references.

College of Arts and Science (CAS)

TIBAYAN, YVES ROY M. "In vitro Screening of Low-density Polyethylenebiodegrading Filamentous Fungi Isolated from Soils in Indang, Cavite". Undergraduate Thesis. Bachelor of Science in Biology major in Microbiology. Department of Biological Sciences, College of Arts and Sciences, Cavite State University, Indang, Cavite. May 2017. Adviser: Prof. Sherine M. Cruzate.
Low-density polyethylene (LDPE) plastics are substantial wellsprings of pollution as they are recalcitrant and bioinert materials; thus, they are escalating incessantly in the
environment. Not only the Philippines, but also the other countries have an immense need to search for efficient and economical approach that would be vastly used to degrade these pollutants.
The present work is on the study of LDPE biodegradation by filamentous fungi
obtained from different sampling locations in Indang, Cavite. The isolates were first screened for their biodegrading ability based on abundant growths on Mineral Salt Agar (MSA) amended with LDPE powder. Out of 58 isolates, 28 yielded positive results with abundant growths on MSA after a 30-day incubation. Thereafter, they were subjected to in vilro biodegradation assay on Mineral Salt Broth (MSB) containing LDPE plastic strips for 30 days. The 24 filamentous fungal isolates were phenotypically characterized and were identified belonging to four genera, viz. Aspergillus, Penicillium, Paecilomyces and Helminthosporium; four were not identified.
The three most promising isolates which exhibited more than 20% biodegradation rates were identified as Aspergillus niger, Paecilomyces sp. and Aspergillus niger with
38.70%, 35.96% and 28.78% rates of biodegradation, correspondingly

Submitted copy to the University Library. 08/03/2017 T-6879