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PCR detection of mt-rrs gene and Rickettsia spp. among ticks collected from cattle and pasture in Cavite / by Jonathan A. Babelonia.

By:

Babelonia, Jonathan A [author]

Contributor(s):

Mago, Emmanuel D [adviser]

Material type: Text

TextLanguage: English Publication details: Indang, Cavite : 2017. Cavite State University- Main Campus,Description: xiii, 53 pages : illustrations ; 28 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Subject(s):

DDC classification:

595.4 B11 2017

Online resources:

Click here to view thesis abstract and table of contents

Production credits:

College of Veterinary Medicine and Biomedical Sciences (VETMET)

Abstract: BABELONIA, JONATHAN A.,PCR Detection of Mt-rrs Gene and Rickettsia spp. among Ticks Collected from Cattle and Pasture in Cavite. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite,2017. Adviser: Dr Emmanuel Mago A study was conducted to have a preliminary survey of the occurrence of Rickettsia pathogens present in Cavite using molecular detection. Tick borne pathogen is of human and animal health concern. Determination of the detection rate of Rickettsia sp. is one of the main objectives of the study. Two hundred sixty-four 264 ticks were collected during the months of April and May. Ticks collected were classified according to species, sex and development stage. DNA samples were extracted using two methods boiling and black Prep Kit. Samples extracted using the kit showed higher detection rate of meters gene. One hundred sixty-five (165) DNA samples were detected having a meter’s gene screening and proceeded for the detection of pathogens. Detection of rickettsia pathogens were done via amplification of citrate synthase (gltA) gene using a nested PCR. All tick samples showed negative results in the test conducted. Results showed that no distinct bands were present in the target 500 bp. It is highly suggestive that cattle farms from Cavite has zero to very low detection rate of rickettsia pathogens .

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Holdings ( 1 )
Title notes ( 5 )

Holdings
Item type	Current library	Collection	Call number	Materials specified	URL	Status	Notes	Date due	Barcode
Theses / Manuscripts	Ladislao N. Diwa Memorial Library Theses Section	Non-fiction	595.4 B11 2017 (Browse shelf(Opens below))		Link to resource	Room use only	T-6821		00011273

Thesis (Doctor of Veterinary Medicine) Cavite State University

Includes bibliographical references.

College of Veterinary Medicine and Biomedical Sciences (VETMET)

BABELONIA, JONATHAN A.,PCR Detection of Mt-rrs Gene and Rickettsia spp. among Ticks Collected from Cattle and Pasture in Cavite. Undergraduate Thesis. Doctor of Veterinary Medicine. Cavite State University, Indang, Cavite,2017. Adviser: Dr Emmanuel Mago

A study was conducted to have a preliminary survey of the occurrence of Rickettsia pathogens present in Cavite using molecular detection. Tick borne pathogen is of human and animal health concern. Determination of the detection rate of Rickettsia sp. is one of the main objectives of the study. Two hundred sixty-four 264 ticks were collected during the months of April and May. Ticks collected were classified according to species, sex and development stage. DNA samples were extracted using two methods boiling and black Prep Kit. Samples extracted using the kit showed higher detection rate of meters gene. One hundred sixty-five (165) DNA samples were detected having a meter’s gene screening and proceeded for the detection of pathogens. Detection of rickettsia pathogens were done via amplification of citrate synthase (gltA) gene using a nested PCR. All tick samples showed negative results in the test conducted. Results showed that no distinct bands were present in the target 500 bp. It is highly suggestive that cattle farms from Cavite has zero to very low detection rate of rickettsia pathogens .

Submitted copy to the University Library. 08-01-2017 T-6821